W
Wouter de Laat
Researcher at Utrecht University
Publications - 124
Citations - 21590
Wouter de Laat is an academic researcher from Utrecht University. The author has contributed to research in topics: Chromatin & Enhancer. The author has an hindex of 67, co-authored 119 publications receiving 19333 citations. Previous affiliations of Wouter de Laat include Royal Netherlands Academy of Arts and Sciences & Erasmus University Medical Center.
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Journal ArticleDOI
Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions
Lars Guelen,Ludo Pagie,Emilie Brasset,Wouter Meuleman,Wouter Meuleman,Marius B. Faza,Wendy Talhout,Bert H.J. Eussen,Annelies de Klein,Lodewyk F. A. Wessels,Lodewyk F. A. Wessels,Wouter de Laat,Bas van Steensel +12 more
TL;DR: A high-resolution map of the interaction sites of the entire genome with NL components in human fibroblasts is constructed and demonstrates that the human genome is divided into large, discrete domains that are units of chromosome organization within the nucleus.
Journal ArticleDOI
Looping and interaction between hypersensitive sites in the active β-globin locus
TL;DR: It is proposed that clustering of regulatory elements is key to creating and maintaining active chromatin domains and regulating transcription.
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Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture–on-chip (4C)
Marieke Simonis,Petra Klous,Erik Splinter,Yuri M. Moshkin,Rob Willemsen,Elzo de Wit,Bas van Steensel,Wouter de Laat +7 more
TL;DR: It is demonstrated here that active and inactive genes are engaged in many long-range intrachromosomal interactions and can also form interchromosomal contacts and establish 4C technology as a powerful tool to study nuclear architecture.
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A decade of 3C technologies: insights into nuclear organization
Elzo de Wit,Wouter de Laat +1 more
TL;DR: The current 3C-based methods are evaluated and compared, their contribution to the current understanding of genome structure is summarized, and how shape influences genome function is discussed.
Journal ArticleDOI
Quantitative analysis of chromosome conformation capture assays (3C-qPCR)
Hélène Hagège,Petra Klous,Caroline Braem,Erik Splinter,Job Dekker,Guy Cathala,Wouter de Laat,Thierry Forné +7 more
TL;DR: This work has adapted the real-time TaqMan PCR technology to the analysis of 3C assays, resulting in a method that more accurately determines crosslinking frequencies than current semiquantitative 3C strategies that rely on measuring the intensity of ethidium bromide-stained PCR products separated by gel electrophoresis.