scispace - formally typeset
Search or ask a question
Author

Wright Dj

Bio: Wright Dj is an academic researcher. The author has contributed to research in topics: Nanopore sequencing & Cellular differentiation. The author has co-authored 1 publications.
Topics: Nanopore sequencing, Cellular differentiation, Alternative splicing, RNA splicing
Papers
More filters
Posted Content•DOI•
Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes

[...]

Wright Dj, Nicholas Hall1, Nicholas Hall2, N Irish, Angela L. Man, Glynn W, Arne W. Mould1, Arne W. Mould2, De Los Angeles A2, De Los Angeles A1, E Angiolini, David Swarbreck, Karim Gharbi, Elizabeth M. Tunbridge2, Elizabeth M. Tunbridge1, Wilfried Haerty •
University of Oxford1, Oxford Health NHS Foundation Trust2
28 Apr 2021-bioRxiv
TL;DR: In this article, a custom annotation of a well-studied human neuroblastoma cell line was used to identify isoform expression and usage across differentiation, including a novel transcript of the voltage-gated calcium channel subunit gene.
Abstract: Alternative splicing (AS) is a key mechanism underlying cellular differentiation and a driver of complexity in mammalian neuronal tissues. However, understanding of which isoforms are differentially used or expressed and how this affects cellular differentiation remains unclear. Long read sequencing allows full-length transcript recovery and quantification, enabling transcript-level analysis of AS processes and how these change with cell state. Here, we utilise Oxford Nanopore Technologies sequencing to produce a custom annotation of a well-studied human neuroblastoma cell line and to characterise isoform expression and usage across differentiation. We identify many previously unannotated features, including a novel transcript of the voltage-gated calcium channel subunit gene, CACNA2D2. We show differential expression and usage of transcripts during differentiation, and identify a putative molecular regulator underlying this state change. Our work highlights the potential of long read sequencing to uncover previously unknown transcript diversity and mechanisms influencing alternative splicing.