Xiangqian Ma

TL;DR: The results suggest that sRNA selection of target mRNAs and regulatory hierarchy are influenced by several molecular features and that the combination of these features precisely tunes the efficiency of regulation of multi‐target sRNA regulons.

TL;DR: In this article, the mutants of bacterial small RNA, SgrS, and their regulation of ptsG mRNA target were investigated using Sort-Seq and super-resolution imaging to investigate in vivo target recognition and rejection kinetics.

TL;DR: In this paper, a purified RNA-binding protein (RBP) from Bacteroides thetaiotaomicron was found to be able to bind to single-stranded RNA in vitro with an affinity similar to other characterized RBPs.

TL;DR: In this article, a general approach combining imaging and mathematical modeling is presented to determine kinetic parameters at different levels of sRNA-mediated gene regulation that contribute to overall regulation efficacy, and it is shown that certain sRNAs act post-transcriptionally by affecting translation and degradation of the target mRNAs upon basepairing interactions.

TL;DR: High-throughput sequencing is used to identify functionally relevant single point mutants of the bacterial sRNA, SgrS, and quantitative super-resolution microscopy to probe the mutational impact on the regulation of its primary target, ptsG mRNA, and shows an in vivo demonstration that Hfq directly facilitates sRNA-mRNA annealing.