Xiao-Qiang Wang

Bio: Xiao-Qiang Wang is an academic researcher from Kongju National University. The author has contributed to research in topics: Association mapping & Genetic distance. The author has an hindex of 2, co-authored 2 publications receiving 17 citations.

Comparison of Population Genetic Structures between Asian and American Mungbean Accessions Using SSR Markers

Abstract: The purpose of this study was to evaluate the genetic diversity and population structure of 65 mungbean accessions collected from East and Southeast Asia, the United States and Guatemala using 15 simple sequence repeat (SSR) markers. In total, 47 alleles were detected, the number of the alleles per locus range from two to six, with an average of 3.13. The mean major allele frequency (MAF), expected heterozygosity (HE), and polymorphic information content (PIC) of the 15 SSR loci were 0.76, 0.05, and 0.28, respectively. Of the 47 alleles, 17 (36.2%) were common, with a frequency of 0.05– 0.5; 16 (34.0%) were rare (frequency 0.5). On the basis of the UPGMA dendrogram, most of the accessions were clustered into two main groups. The first group (Group I) included seven accessions and the second comprised 58 accessions, which were further divided into four subgroups. Four subpopulations were detected by model-based structure analysis. Fifty-five accessions (84.6%) showed a clear relation to each cluster based on their inferred ancestry value (>75%), while the remaining 10 accessions (15.4%) were categorized as admixtures. Mungbean accessions from US distributed to almost all clusters and 2 accessions shared genetic constituents showing it derived from mixed ancestry with Asean accessions. These results could be useful in identifying mungbean germplasms and facilitating their improvement programs.

Genome scanning of Asiatic Vigna species for discerning population genetic structure based on microsatellite variation

Abstract: Wild relatives are important genetic resources for crop improvement. However, basic information about their population structure, genetic diversity, species relationships and distribution of variation in a gene pool remains scanty in Vigna species. The level of genetic diversity and population genetic structure of representative accessions of cultivated and wild Asiatic Vigna species collected from diversity-rich endemic areas of India have been investigated using both microsatellite markers and morphological descriptors. Forty-one wild and 12 cultivated accessions of 13 Vigna species were genotyped using 53 polymorphic microsatellite markers. A total of 539 alleles were detected among 53 accessions at all loci with an average 10.16 alleles per locus. The major allele frequency varied from 0.16 to 0.65 (mean = 0.30), while polymorphism information content of polymorphic markers ranged from 0.47 to 0.89 (mean = 0.79). The UPGMA revealed five major clusters accommodating ~96 % of the accessions. The largest cluster accommodated 19 (36 %), while the smallest cluster had only two accessions. Two accessions, JAP/10-5 and JAP/10-9 of V. trilobata, did not group with any other accession. The model-based population structure analysis also showed almost similar pattern and grouped 53 accessions of Vigna into five genetically distinct sub-populations (K = 5) based on maximum ∆K values. Duncan’s multiple range test revealed significant difference between five genetic and one admixture group developed through population structure analysis with 22 morphological descriptors. Analysis of variance for morphological data revealed significant difference in 12 qualitative and quantitative traits including growth habit, terminal leaflet length, colour of petiole base, petiole length, leaf senescence, length of peduncle, raceme position, calyx colour, colour of VSI pod, pod pubescence, pod curvature and 100-seed weight, indicating their significance in distinguishing population groups. The information on genetic diversity and population structure of wild and cultivated accessions of Asiatic Vigna will be tremendously useful to accelerate their use in trait improvement.

Cross-genera amplification of informative microsatellite markers from common bean and scarlet runner bean for assessment of genetic diversity in mungbean (Vigna radiata)

Abstract: Mungbean (V. radiata) is an important Asiatic legume supplying inexpensive protein to a vast majority of vegetarian masses. To increase markers repertoire in mungbean, a study was conducted to analyse 384 microsatellite markers derived from common bean, scarlet runner bean and adzuki bean for their transferability and polymorphism. The results showed that 87 (24.71%) primer pairs could amplify DNA loci of 20 mungbean genotypes including one accession of V. trilobata, while 52 showed reliable banding and polymorphism. These showed different degrees of variability at each locus producing 250 alleles with the number of alleles varying from 2 to 9. The major allele frequency varied from 0.17 to 0.95, while the polymorphic information content of SSRs ranged between 0.09 and 0.86 with an average of 0.60 ± 0.16. UPGMA revealed three major clusters accommodating ~95% of the accessions while one accession of V. trilobata (‘NSB-007’) did not group with any other genotype describing the discriminating power of informative microsatellites. This study identified a set of useful microsatellite markers to accelerate the genetic studies and breeding programme of mungbean.