Author
Xiaoqin Wang
Other affiliations: Soochow University (Suzhou), Massachusetts Institute of Technology
Bio: Xiaoqin Wang is an academic researcher from Tufts University. The author has contributed to research in topics: Fibroin & SILK. The author has an hindex of 23, co-authored 39 publications receiving 5601 citations. Previous affiliations of Xiaoqin Wang include Soochow University (Suzhou) & Massachusetts Institute of Technology.
Topics: Fibroin, SILK, Medicine, Self-healing hydrogels, Materials science
Papers
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TL;DR: This protocol includes methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films, used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, as well as for drug delivery.
Abstract: Silk fibroin, derived from Bombyx mori cocoons, is a widely used and studied protein polymer for biomaterial applications. Silk fibroin has remarkable mechanical properties when formed into different materials, demonstrates biocompatibility, has controllable degradation rates from hours to years and can be chemically modified to alter surface properties or to immobilize growth factors. A variety of aqueous or organic solvent-processing methods can be used to generate silk biomaterials for a range of applications. In this protocol, we include methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films. These materials can be used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, as well as for drug delivery.
2,165 citations
TL;DR: Sonication provides a useful new tool with which to initiate rapid sol-gel transitions, such as for cell encapsulation, with high ionic strength and temperature and low pH responsible for increasing gelation kinetics.
572 citations
TL;DR: The silk I structure, a key intermediate secondary structure for the formation of mechanically robust natural silk fibers, was successfully generated by the present approach of very slow drying, mimicking the natural process, and point to a new mode of generating new types of silk biomaterials with enhanced mechanical properties and increased degradation rates, while maintaining water insolubility.
556 citations
TL;DR: A novel silk microsphere/scaffold system offers a new option for the delivery of multiple growth factors with spatial control in a 3D culture environment for both understanding natural tissue growth process and in vitro engineering complex tissue constructs.
421 citations
TL;DR: A new aqueous-based preparation method for silk spheres with controllable sphere size and shape is reported, based on phase separation between silk fibroin and polyvinyl alcohol (PVA) at a weight ratio of 1/1 and 1/4.
378 citations
Cited by
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TL;DR: This review will provide a comprehensive overview of general properties of alginate and its hydrogels, their biomedical applications, and suggest new perspectives for future studies with these polymers.
5,372 citations
TL;DR: This protocol includes methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films, used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, as well as for drug delivery.
Abstract: Silk fibroin, derived from Bombyx mori cocoons, is a widely used and studied protein polymer for biomaterial applications. Silk fibroin has remarkable mechanical properties when formed into different materials, demonstrates biocompatibility, has controllable degradation rates from hours to years and can be chemically modified to alter surface properties or to immobilize growth factors. A variety of aqueous or organic solvent-processing methods can be used to generate silk biomaterials for a range of applications. In this protocol, we include methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films. These materials can be used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, as well as for drug delivery.
2,165 citations
TL;DR: A material strategy for a type of bio-interfaced system that relies on ultrathin electronics supported by bioresorbable substrates of silk fibroin that provides new capabilities for implantable and surgical devices is described.
Abstract: Electronics that are capable of intimate, non-invasive integration with the soft, curvilinear surfaces of biological tissues offer important opportunities for diagnosing and treating disease and for improving brain/machine interfaces. This article describes a material strategy for a type of bio-interfaced system that relies on ultrathin electronics supported by bioresorbable substrates of silk fibroin. Mounting such devices on tissue and then allowing the silk to dissolve and resorb initiates a spontaneous, conformal wrapping process driven by capillary forces at the biotic/abiotic interface. Specialized mesh designs and ultrathin forms for the electronics ensure minimal stresses on the tissue and highly conformal coverage, even for complex curvilinear surfaces, as confirmed by experimental and theoretical studies. In vivo, neural mapping experiments on feline animal models illustrate one mode of use for this class of technology. These concepts provide new capabilities for implantable and surgical devices. Electronics that are capable of intimate integration with the surfaces of biological tissues create opportunities for improving animal/machine interfaces. A bio-interfaced system of ultrathin electronics supported by bioresorbable silk-fibroin substrates is now presented. Mounting such devices on tissue and then allowing the silk to dissolve initiates a conformal wrapping process that is driven by capillary forces.
1,522 citations
TL;DR: The most relevant biopolymer-based hydrogel systems, the different methods of preparation, as well as an in depth overview of the applications in the field of tissue engineering will be given.
1,426 citations
TL;DR: This article provides a comprehensive review on the elastomeric biomaterials used in tissue engineering, with emphasis on the most important candidates to date.
1,051 citations