Author
Xinsheng Zhang
Bio: Xinsheng Zhang is an academic researcher. The author has contributed to research in topics: Pyrococcus horikoshii & Hydrolase. The author has an hindex of 1, co-authored 2 publications receiving 3 citations.
Papers
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Patent•
21 Jan 2009
TL;DR: In this article, a method for quickly breeding a temperature-sensitive recessive male-sterile gene homozygote by utilizing a castor pollen regeneration system and hybridizing a new castor species by the temperature sensitive recessive MRGE homozygotes, which is taken as a female parent, and a selfing line male parent.
Abstract: The invention relates to a high-efficiency castor isofemale line creating method and a approach of applying the castor isofemale line to castor hybridization, and in particular relates to a method for quickly breeding a temperature-sensitive recessive male-sterile gene homozygote by utilizing a castor pollen regeneration system and hybridizing a new castor species by the temperature-sensitive recessive male-sterile gene homozygote, which is taken as a female parent, and a selfing line male parent. By adopting the method of the invention, a large amount of the temperature-sensitive male-sterile isofemale line germ plasm can be high-efficiently created quickly; and what is more important is that the temperature-sensitive male-sterile isofemale line germ plasm is taken as the breeding female parent to breed the new castor species with properties of high yield, high oil content, multiple resistance, no fruit fall, no capsule cracking, full seed, high kernel rate, etc.
2 citations
Patent•
05 Apr 2017
TL;DR: In this paper, a polysaccharide hydrolase gene from pyrococcus horikoshii and its application was revealed, and a recombinant vector of the hydrolases was constructed and excessively expressed in pichia pastoris.
Abstract: The invention belongs to the technical field of bioengineering and enzyme engineering, and discloses a polysaccharide hydrolase gene from pyrococcus horikoshii and application. A nucleotide sequence of the polysaccharide hydrolase gene Tk1765 is shown as SEQ ID NO:1. A coding sequence of the polysaccharide hydrolase gene Tk1765 is shown as SEQ ID NO:2. Polysaccharide hydrolase has the efficient cellulose gum and chitose double digest character. According to cellulose gum of the polysaccharide hydrolase, the optimum pH value is 10.0, and the optimum enzyme reaction temperature is 65 DEG C. According to hydrolysis chitose, the optimum pH value is 11.0, and the optimum enzyme reaction temperature is 80 DEG C. The hydrolase is heated for 4 h at the temperature being 100 DEG C, and the activity of the hydrolase can be kept to be 40% or above. A recombinant vector of the hydrolase is constructed and excessively expressed in pichia pastoris, a product of the hydrolase has the function of hydrolyzing maize straw, and the polysaccharide hydrolase and the coding gene thereof can be widely applied to cellulose and chitin degradation.
1 citations
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Patent•
31 Jul 2018
TL;DR: In this paper, a chitin enzyme with a high efficiency degradation rate was obtained through cloning a gene, which was used for coding the chITin enzyme, in a genome of streptomyces albolongus ATCC (American Type Culture Collection) 27414, constructing a recombinant vector and transferring the recombinant vectors into escherichia coli BL21 (DE3) to carry out inducible expression.
Abstract: The invention aims at providing a novel chitin enzyme with a high-efficiency degradation rate. The high-activity soluble chitin enzyme is obtained through cloning a gene, which is used for coding thechitin enzyme, in a genome of streptomyces albolongus ATCC (American Type Culture Collection) 27414, constructing a recombinant vector and transferring the recombinant vector into escherichia coli BL21 (DE3) to carry out inducible expression. Through Ni column affinity chromatography, the chitin pure enzyme is purified and obtained; the enzymatic property and hydrolysis mechanism of the chitin pure enzyme are researched; the optimum temperature and optimum pH (potential of Hydrogen) of the enzyme are 55 DEG C and 5.0 respectively; the specific enzyme activity is 66.2U/mg; a main product of degraded chitin is chitobiose, and the chitin enzyme is expected to be applied to the preparation of a chitooligosaccharide, and has potential application value.
3 citations
Patent•
20 Feb 2013
TL;DR: In this article, the axillary buds of ricinus communist female plants are adopted as a material, and is washed by using running water; sterilizing is carried out; induced differentiation culture is carried on axillary bud, proliferation culture, and rooting culture are carried out.
Abstract: The invention discloses a breeding maintaining method of ricinus communist female plants. According to the invention, ricinus communist female plants are subjected to tissue culturing, such that ricinus communist female plant breeding maintenance is realized. The tissue culturing process comprises the steps that: (1) axillary buds of ricinus communist female plants are adopted as a material, and is washed by using running water; (2) sterilizing is carried out; (3) induced differentiation culture is carried out; (4) proliferation culture is carried out; and (5) rooting culture is carried out. With the method provided by the invention, a survival rate of female plant axillary bud in-vitro culture is high, a propagation speed is high, and a multiplication coefficient reaches 5.88.
2 citations
Patent•
26 Apr 2017
TL;DR: In this article, a castor-oil plant tissue culture plants concentrated propagation method was used for tissue culture plant propagation of different lines, where castor oil plants of different line were cultured in a concentrated manner, and the castoroil plants of each line in a squaring stage were placed in an isolation chamber so that the castors of different labels will not affect each other.
Abstract: The invention provides a castor-oil plant tissue culture plants concentrated propagation method, which is used for tissue culture plants propagation of different lines. Castor-oil plants of different lines are cultured in a concentrated manner, and the castor-oil plants of each line in a squaring stage are placed in an isolation chamber so that the castor-oil plants of different lines will not affect each other. Concentrated seedling cultivation is carried out, the plants need not to be planted in separated different areas, the planting efficiency is improved, and observation and recording in a phonological period is convenient. Isolation is conducted in the squaring stage to ensure that cross infection among the plants will not happen, therefore, the survival rate and the reproduction rate are increased.