Y. D. Wang

Bio: Y. D. Wang is an academic researcher from Fooyin University. The author has contributed to research in topics: Cinnamomum subavenium & Vanillic acid. The author has an hindex of 6, co-authored 8 publications receiving 144 citations.

Chemical constituents from the leaves of Michelia alba

Abstract: The genus Michelia (Magnoliaceae) consists of about 30 species. Michelia alba is an evergreen tree, especially distributed in Taiwan and China. Michelia species have been used by indigenous peoples for the treatment of cancer. For example, Michelia champaca has been used in India for the treatment of abdominal tumors and M. hypoleuca and M. officinalis for carcinomatous sores and leukemia, respectively, in China [1]. Previous phytochemical studies on this species isolated, from the roots and flowers of M. alba, ushinsunine, oxoushinsunine, salicifoline, michelalbine, limonene, benzyl acetate, linalool, nerol, hydroxycitronellal, benzaldehyde, benzyl benzoate, and methyl eugenol [2, 3]. To further understand the chemotaxonomy and to continue searching for novel agents from Magnoliaceous plants, the leaves of M. albawere chosen for the first time for phytochemical investigation. In this paper, we report the isolation of 21 pure substances. The compounds included four aporphines, (-)-anonaine ( 1) [4], (-)-norushinsunine ( 2) [5], (-)-ushinsunine ( 3) [5], and (-)-N-acetylanonaine ( 4) [6]; two oxoaporphines, liriodenine ( 5) [5] and oxoxylopine ( 6) [7]; three sesquiterpene lactones, michelenolide ( 7) [8], costunolide ( 8) [8], and 11,13-dehydrolanuginolide ( 9) [9]; one amide, N-trans-feruloyltyramine ( 10) [10]; one lignan, (+)-syringaresinol ( 11) [11]; three benzenoids, 4-hydroxybenzaldehyde ( 12) [12], 4-hydroxybenzoic acid ( 13) [12], and methylparaben ( 14) [12]; two steroids, β-sitosterol and stigmasterol [13]; three aliphatic compounds, palmitic acid [14], stearic acid [15], and linoleic acid [16]; and two chlorophylls, pheophorbide a [17] and aristophyll-C [18]. In addition to 3 and 5, all of these compounds were isolated for the first time from this source.

A novel sesquiterpenoid from the leaves of Cinnamomum subavenium

Abstract: A novel sesquiterpenoid, subamol ((3-methoxy-5H-9,10-dihydroxybenzo[3,4]cyclohepta[1,2-f])inden-7-yl)methanol)) (1), along with six compounds, including one ionone: (+)-abscisic acid (2); and five benzenoids: syringaldehyde (3), trans-coumaric acid (4), cis-coumaric acid (5), vanillic acid (6), and p-hydroxybenzoic acid (7), were isolated from the leaves of Cinnamomum subavenium Miq (Lauraceae). These compounds were identified and characterized by physical and spectral evidence.

Chemical Constituents from the Roots of Synsepalum dulcificum.

Abstract: Botanical data and some properties of Synsepalum dulcificum Daniell (Sapotaceae) are discussed in our article published in the journal number [1]. The MeOH extract of its roots was subjected to solvent partitioning and chromatographic separation to afford five fractions. The chemical constituents in the roots of S. dulcificum were separated with column chromatography. Nine compounds, including N-trans-feruloyltyramine (1) [2], N-cis-feruloyltyramine (2) [3], N-trans-feruloylmethoxytyramine (3), N-cis-feruloylmethoxytyramine (4) [4], p-hydroxybenzoic acid (5), methylparaben (6), vanillic acid (7), isovanillic acid (8), and syringic acid (9) [5], were isolated from the roots of S. dulcificum. All of these compounds were found for the first time from this plant. The specimen of S. dulcificum was collected from Kaohsiung County, Taiwan, October 2007. A voucher specimen was identified by Dr. Fu-Yuan Lu (Department of Forestry and Natural Resources College of Agriculture, National Chiayi University) and deposited in the School of Medical and Health Science, The Fooyin University, Kaohsiung County, Taiwan. The roots (5.2 kg) of S. dulcificum were extracted repeatedly with MeOH at room temperature for 24–48 hrs. The MeOH extract was dried and evaporated to leave a viscous residue (43.8 g). The residue was placed on a silica gel column and eluted with CHCl3 gradually enriched with MeOH to afford five fractions. Fraction 2 (5.11 g) eluted with n-hexane–EtOAc (1:1) was further purified by silica gel CC using the same solvent system to obtain N-trans-feruloyltyramine (1) (8 mg) and N-cisferuloyltyramine (2) (5 mg). Fraction 3 (7.81 g) eluted with n-hexane–Me2CO (6:1) was further separated using silica gel CC and preparative TLC (n-hexane–EtOAc (1:2)) to give N-trans-feruloylmethoxy-tyramine (3) (18 mg) and N-cisferuloylmethoxytyramine (4) (12 mg). Fraction 4 (3.12 g) was purified by silica gel chromatography (CHCl3–MeOH, 80:1) to give p-hydroxybenzoic acid (5) (4 mg) and methylparaben (6) (6 mg). Fraction 5 (5.91 g) was purified by silica gel chromatography (CHCl3–MeOH, 50:1) to give colorless needles of vanillic acid (7) (15 mg), isovanillic acid (8) (10 mg), and syringic acid (9) (22 mg). N-trans-Feruloyltyramine (1) as in [2], colorless crystals (CHCl3), UV ( max, nm): 220, 293, 319. IR ( max, cm –1): 3300 (OH), 1650 (C=O). 1H NMR (400 MHz, CDCl3, , ppm, J/Hz): 2.75 (2H, t, J = 6.8, H-2), 3.54 (2H, t, J = 6.8, H-1), 3.87 (3H, s, OCH3), 6.15 (1H, d, J = 15.6, H-2 ), 6.75 (2H, d, J = 8.8, H-5 and H-7), 6.83 (1H, d, J = 8.0, H-8 ), 7.00 (1H, dd, J = 8.0, 2.0, H-9 ), 7.02 (2H, d, J = 8.8, H-4 and H-8), 7.46 (1H, d, J = 15.6, H-3 ), EI-MS m/z: 313 [M]+. N-cis-Feruloyltyramine (2) as in [3], yellow oil, UV ( max, nm): 220, 293, 318. IR ( max, cm –1): 3350 (OH), 1650 (C=O). 1H NMR (400 MHz, CD3OD, , ppm, J/Hz): 2.69 (2H, t, J = 7.6, H-2), 3.40 (2H, t, J = 7.6, H-1), 3.83 (3H, s, OCH3), 5.81 (1H, d, J = 12.8, H-2 ), 6.61 (1H, d, J = 12.8, H-3 ), 6.68 (2H, d, J = 8.4, H-5 and H-7), 6.73 (1H, d, J = 8.4, H-8 ), 6.92 (1H, dd, J = 8.4, 2.0, H-9 ), 6.99 (2H, d, J = 8.4, H-4 and H-8), 7.35 (1H, d, J = 2.0, H-5 ), EI-MS m/z: 313 [M]+.

Chemical constituents from the leaves of Synsepalum dulcificum

Abstract: Daniell (Sapotaceae) is an evergreen shrub native to tropical West Africa, and the fruits, andred berries have the property of modifying sour taste into sweet taste remarkably. Interestingly, the active material of the be rryis the protein, miraculin, which has no taste in itself. The sweetness intensity induced by 0.02 M citric acid after 0.4 M puri fiedmiraculin solution is held in the mouth is equivalent to that of about 0.3 M sucrose. This value is equal to the maximumsweetness induced by miraculin [1].The specimen of

Norcadinane sesquiterpene from the roots of Cinnamomum subavenium

Abstract: 0009-3130/11/4703-0461 2011 Springer Science+Business Media, Inc. School of Medical and Heath Science, The Fooyin University, Ta-Liao, Kaohuing, Taiwan 831, R. O. C., fax: 886 7 7863667, e-mail: xx377@mail.fy.edu.tw. Published in Khimiya Prirodnykh Soedinenii, No. 3, pp. 409–410, May–June, 2011. Original article submitted March 12, 2010. Chemistry of Natural Compounds, Vol. 47, No. 3, July, 2011 [Russian original No. 3, May–June, 2011]

Phytosynthesis of silver nanoparticles (AgNPs) using miracle fruit plant (Synsepalum dulcificum) for antimicrobial, catalytic, anticoagulant, and thrombolytic applications

Abstract: Abstract In the present work, we report the phytosynthesis of AgNPs mediated by leaf and seed extracts of Synsepalum dulcificum. The extracts catalyzed the formation of brown colloidal AgNPs, which stabilized in 10 min. The leaf and seed AgNPs yielded surface plasmon resonance at 440 and 438.5 nm, respectively. Prominent peaks at 3408, 2357, 2089, and 1639 cm−1 were recorded for leaf AgNPs, whereas 3404, 2368, 2081, and 1641 cm−1 were revealed for seed-mediated AgNPs from Fourier transform infrared data. These showed the involvement of phenolic compounds and proteins in the phytosynthesis. The particles were fairly spherical and crystalline in nature having size of 4–26 nm, with prominence of silver in the colloidal solutions. The particles inhibited the growth of drug-resistant strains of Pseudomonas aeruginosa and Klebsiella granulomatis with zone of inhibition of 11–24 mm. Also, the phytosynthesized AgNPs completely inhibited the growth of Aspergillus flavus and Aspergillus niger. In addition, by using 20 μg/ml of AgNPs, malachite green was degraded by approximately 80% in 24 h. Similarly, the particles displayed blood anticoagulant activities as well as achieved thrombolysis. The AgNPs can be explored for biomedical and catalytic applications. The report is the first on the eco-friendly synthesis of nanoparticles by S. dulcificum.

Bioconstituents from stems of Synsepalum dulcificum Daniell (Sapotaceae) inhibit human melanoma proliferation, reduce mushroom tyrosinase activity and have antioxidant properties

Abstract: A new amide, dihydro-feruloyl-5-methoxytyramine ( 1 ), along with 13 known compounds, including (+)-syringaresinol ( 2 ), (+)- epi -syringaresinol ( 3 ), 4-acetonyl-3,5-dimethoxy- p -quinol ( 4 ), cis - p -coumaric acid ( 5 ), trans - p -coumaric acid ( 6 ), p -hydroxybenzoic acid ( 7 ), syringic acid ( 8 ), vanillic acid ( 9 ), veratric acid ( 10 ), N - cis -feruloyltyramine ( 11 ), N - trans -feruloyltyramine ( 12 ) and N - cis -caffeoyltyramine ( 13 ), were isolated from the stems of Synsepalum dulcificum Daniell (Sapotaceae). The structures of these compounds were established on the basis of spectroscopic analysis. One of the purposes of this study was to survey the antioxidant properties of 13 pure constituents. The radical scavenging and antioxidant activities were investigated by: scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals; metal chelating power was completed by ferrous ions; and reducing power was demonstrated by iron ions reduction reaction. In addition, all compounds were evaluated for their cell proliferation inhibition activities on human skin melanoma cells and tyrosinase inhibitions. The anti-tyrosinase effects were to calculate the hydroxylation of l -tyrosine to l -dopa according to in vitro mushroom tyrosinase assay. In sum, the inhibition effects of compounds ( 2 – 3 ) on human melanoma cells were significant. Besides, DPPH, ABTS radical scavenging, metal chelating and reducing power were found to be moderate compared with the positive controls.