Yaara Oren

TL;DR: The extent, origins and consequences of genetic variation within human cell lines are studied, providing a framework for researchers to measure such variation in efforts to support maximally reproducible cancer research.

TL;DR: This work shows that somatic mutations in mtDNA can be tracked by single-cell RNA or assay for transposase accessible chromatin (ATAC) sequencing and leverages somatic mtDNA mutations as natural genetic barcodes and demonstrates their utility as highly accurate clonal markers to infer cellular relationships.

TL;DR: This approach reveals the existence of multiple circulating subtypes of the major drug–resistant clade of the important pathogenic E. coli lineage ST131 and provides the first ever population level evidence of core genome substitutions in gene regulatory regions associated with the acquisition and maintenance of different accessory genome elements.

TL;DR: Watermelon as mentioned in this paper is a barcode lentiviral library for simultaneous tracing of each cell's clonal origin and proliferative and transcriptional states, which can identify rare persister lineages that are preferentially poised to proliferate under drug pressure, thus exposing new vulnerabilities that can be targeted to prevent disease recurrence.

TL;DR: It is established that regulatory regions can “switch” between nonhomologous alternatives and that switching is ubiquitous, occurring across the bacterial domain, and that regulatory switching has a strong impact on promoter architecture and expression divergence.