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Author

Yang Yan

Bio: Yang Yan is an academic researcher from Xinxiang Medical University. The author has contributed to research in topics: Detection limit & Endogeny. The author has an hindex of 1, co-authored 1 publications receiving 1 citations.

Papers
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Journal ArticleDOI
TL;DR: The construction of a turn-on fluorescent probe, TMN-H2O2 with near-infrared emission with excellent sensitivity and fast response time is reported, suggesting that this probe is a reliable chemical tool for monitoring both endogenous and exogenous H2 O2.
Abstract: In this study, we report the construction of a turn-on fluorescent probe, TMN-H2O2 with near-infrared emission for H2O2 detection in living systems. A series of experiments demonstrated that TMN-H2O2 exhibits a high selectivity for H2O2. After reaction with H2O2, TMN-H2O2 exhibited a 14.3-fold increase in fluorescence intensity at 660 nm and a 180 nm large Stokes shift. Moreover, TMN-H2O2 exhibited excellent sensitivity (limit of detection: 76 nM) and a fast response time (~40 min). The successful in vitro and in vivo application of TMN-H2O2 therefore suggested that this probe is a reliable chemical tool for monitoring both endogenous and exogenous H2O2.

16 citations


Cited by
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Journal ArticleDOI
TL;DR: In this paper , the first naphthalimide-triphenylamine-based dual-site fluorescent probe NATPA was developed for simultaneously imaging intracellular H2O2 and polarity fluctuations.
Abstract: H2O2 and polarity are quite important in many physiological and pathological processes, and their relationship is complicated and obscure for researchers. Thus, it is vital and challenging to achieve simultaneous detection of H2O2 and polarity in vivo. Herein, the first naphthalimide-triphenylamine-based dual-site fluorescent probe NATPA is developed for simultaneously imaging intracellular H2O2 and polarity fluctuations. It exhibits excellent sensitivity (LOD = 44 nM), selectivity, and fast response (15 min) to H2O2 and a superior capacity for detecting polarity upon the intramolecular charge transfer (ICT) effect. Besides, the probe displays low cytotoxicity and lipid droplet targeting and is further applied in imaging H2O2 and polarity fluctuations in HepG2 and L-02 cells, so that NATPA is qualified to distinguish cancer cells from normal cells. This research contributes a new design principle for the construction of dual-site fluorescent probes for simultaneously detecting active molecules and polarity.

10 citations

Journal ArticleDOI
TL;DR: In this article , a large stokes shift and near-infrared (NIR) probe DCM-HNU was designed and synthesized for visualization of H 2 O 2 in both living HeLa cells and zebra fish.

8 citations

Journal ArticleDOI
TL;DR: In this article, the authors designed and synthesized a large stokes shift and near-infrared (NIR) probe DCM-HNU for visualization of H2O2 in both living HeLa cells and zebra fish.

8 citations

Journal ArticleDOI
TL;DR: The designed electrochemical microsensor provided a ratiometric strategy for real-time tracking of H2O2 in a linear range of 0.5-600 μM with high selectivity and accuracy and was successfully applied to the measurement of H1N2 in Parkinson's disease (PD) mouse brain.
Abstract: Hydrogen peroxide (H2O2), one of the most stable and abundant reactive oxygen species (ROS), acting as a modulator of dopaminergic signaling, has been intimately implicated in Parkinson's disease, creating a critical need for the selective quantification of H2O2 in the living brain. Current natural or nanomimic enzyme-based electrochemical methods employed for the determination of H2O2 suffer from inadequate selectivity and stability, due to which the in vivo measurement of H2O2 in the living brain remains a challenge. Herein, a series of 5-(1,2-dithiolan-3-yl)-N-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pentanamide (DBP) derivatives were designed by tuning the substitute groups and sites of a boric acid ester, which served as probes to specifically react with H2O2. Consequently, the reaction products, 5-(1,2-dithiolan-3-yl)-N-(4-hydroxyphen-yl)pentanamide (DHP) derivatives, converted the electrochemical signal from inactive into active. After systematically evaluating their performances, 5-(1,2-dithiolan-3-yl)-N-(3-chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pentanamide (o-Cl-DBP) was finally identified as the optimized probe for H2O2 detection as it revealed the fastest reaction time, the largest current density, and the most negative potential. In addition, electrochemically oxidized graphene oxide (EOGO) was utilized to produce a stable inner reference. The designed electrochemical microsensor provided a ratiometric strategy for real-time tracking of H2O2 in a linear range of 0.5-600 μM with high selectivity and accuracy. Eventually, the efficient electrochemical microsensor was successfully applied to the measurement of H2O2 in Parkinson's disease (PD) mouse brain. The average levels of H2O2 in the cortex, striatum, and hippocampus in the normal mouse and PD mouse were systematically compared for the first time.

5 citations