Yang Yang

Bio: Yang Yang is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Medicine & Computer science. The author has an hindex of 171, co-authored 2644 publications receiving 153049 citations. Previous affiliations of Yang Yang include Zhejiang University & Northwest Normal University.

Bio/Abiotic Interface Constructed from Nanoscale DNA Dendrimer and Conducting Polymer for Ultrasensitive Biomolecular Diagnosis

Abstract: For sensors detecting immobilized biomarkers, the interface between the surface and the fluid medium plays an important role in determining the levels of signal and noise in the electrochemical detection process. When protein is directly immobilized on the metal electrode, denaturation of the protein by surface-protein interaction results in low activity and low signal level. A conducting polymer-based interface can prevent the protein conformation change and alleviate this problem. A DNA dendrimer is introduced into the interfacial film on the sensor surface to further improve the sensor performance. DNA dendrimer is a nanoscale dendrite constructed of short DNA sequences, which can be easily incorporated into the abiotic conducting polymer matrix and is biocompatible with most biological species. In this work, DNA dendrimer and polypyrrole (DDPpy) form the bio/abiotic interface on electrochemical sensors. Detection of two salivary protein markers (IL-8 and IL-1beta) and one mRNA salivary marker (IL-8) is used to demonstrate the efficiency of the DDPpy sensor. A limit of detection (LOD) of protein of 100-200 fg mL(-1) is achieved, which is three orders of magnitude better than that without the DNA dendrimer interface. An LOD of 10 aM is established for IL-8 mRNA. The typical sample volume used in the detection is 4 microL, thus the LOD reaches only 25 target molecules (40 yoctomole).

Different effects of LDH-A inhibition by oxamate in non-small cell lung cancer cells

Abstract: // Yang Yang 1,2 , Dan Su 3 , Lin Zhao 2 , Dan Zhang 3 , Jiaying Xu 2 , Jianmei Wan 2 , Saijun Fan 2,4 and Ming Chen 1 1 Department of Radiation Therapy, Zhejiang Cancer Hospital, Hangzhou, China; Zhejiang Key Laboratory of Radiation Oncology, Hangzhou, China 2 School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China 3 Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou, China 4 Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China Correspondence: Ming Chen, email: // Saijun Fan, email: // Keywords : lactate dehydrogenase A, Warburg effect, G0/G1 arrest, autophagy, apoptosis, Akt/mTOR Received : September 23, 2014 Accepted : October 22, 2014 Published : October 22, 2014 Abstract Higher rate of glycolysis has been long observed in cancer cells, as a vital enzyme in glycolysis, lactate dehydrogenase A (LDH-A) has been shown with great potential as an anti-cancer target. Accumulating evidence indicates that inhibition of LDH-A induces apoptosis mediated by oxidative stress in cancer cells. To date, it’s still unclear that whether autophagy can be induced by LDH-A inhibition. Here, we investigated the effects of oxamate, one classic inhibitor of LDH-A in non-small cell lung cancer (NSCLC) cells as well as normal lung epithelial cells. The results showed that oxamate significantly suppressed the proliferation of NSCLC cells, while it exerted a much lower toxicity in normal cells. As previous studies reported, LDH-A inhibition resulted in ATP reduction and ROS (reactive oxygen species) burst in cancer cells, which lead to apoptosis and G 2 /M arrest in H1395 cells. However, when being exposed to oxamate, A549 cells underwent autophagy as a protective mechanism against apoptosis. Furthermore, we found evidence that LDH-A inhibition induced G 0 /G 1 arrest dependent on the activation of GSK-3β in A549 cells. Taken together, our results provide useful clues for targeting LDH-A in NSCLC treatment and shed light on the discovery of molecular predictors for the sensitivity of LDH-A inhibitors.

Graded bulk-heterojunction enables 17% binary organic solar cells via nonhalogenated open air coating.

Abstract: Graded bulk-heterojunction (G-BHJ) with well-defined vertical phase separation has potential to surpass classical BHJ in organic solar cells (OSCs). In this work, an effective G-BHJ strategy via nonhalogenated solvent sequential deposition is demonstrated using nonfullerene acceptor (NFA) OSCs. Spin-coated G-BHJ OSCs deliver an outstanding 17.48% power conversion efficiency (PCE). Depth-profiling X-ray photoelectron spectroscopy (DP-XPS) and angle-dependent grazing incidence X-ray diffraction (GI-XRD) techniques enable the visualization of polymer/NFA composition and crystallinity gradient distributions, which benefit charge transport, and enable outstanding thick OSC PCEs (16.25% for 300 nm, 14.37% for 500 nm), which are among the highest reported. Moreover, the nonhalogenated solvent enabled G-BHJ OSC via open-air blade coating and achieved a record 16.77% PCE. The blade-coated G-BHJ has drastically different D-A crystallization kinetics, which suppresses the excessive aggregation induced unfavorable phase separation in BHJ. All these make G-BHJ a feasible and promising strategy towards highly efficient, eco- and manufacture friendly OSCs. Graded bulk-heterojunction organic solar cell with well-defined vertical phase separation has the potential to surpass the classical counterpart, thus the optimisation of this structure is crucial. Here, the authors reveal solvent selection strategies for optimising morphology of the structure, enabling efficient, eco-friendly, and scalable solar cells.

Efficient nanomaterials for harvesting clean fuels from electrochemical and photoelectrochemical CO2 reduction

Abstract: The excessive utilization of fossil fuels accompanied by large amounts of anthropogenic CO2 emissions have led to adverse global environmental changes and a growing global energy crisis. Hence, converting CO2 into high-value chemical fuels, such as CO, CH4, HCOOH, and CH3OH, through catalysis is one of the most attractive topics in energy conversion. Among various approaches, electrochemical (EC) and photoelectrochemical (PEC) reduction are considered to be promising methods. Over the past decades, research in the area of CO2 EC and PEC reduction has been growing quickly. Herein, highly efficient nanostructured metals, metal alloys, metal oxides, metal-free electrodes, and photoelectrodes developed in recent years are discussed in detail in this review. Additionally, the strategies and mechanisms for improving the faradaic efficiency (FE), current density, and stability of catalysts are also discussed. The challenges and future perspectives for CO2 EC and PEC reduction are also discussed.

Λ Λ correlation function in Au+Au collisions at sNN =200GeV

Abstract: We present Lambda Lambda correlation measurements in heavy-ion collisions for Au + Au collisions at root s(NN) = 200 GeV using the STAR experiment at the Relativistic Heavy-Ion Collider. The Lednicky-Lyuboshitz analytical model has been used to fit the data to obtain a source size, a scattering length and an effective range. Implications of the measurement of the Lambda Lambda correlation function and interaction parameters for dihyperon searches are discussed.

I and i

Abstract: There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality. Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

Fast parallel algorithms for short-range molecular dynamics

Abstract: Three parallel algorithms for classical molecular dynamics are presented. The first assigns each processor a fixed subset of atoms; the second assigns each a fixed subset of inter-atomic forces to compute; the third assigns each a fixed spatial region. The algorithms are suitable for molecular dynamics models which can be difficult to parallelize efficiently—those with short-range forces where the neighbors of each atom change rapidly. They can be implemented on any distributed-memory parallel machine which allows for message-passing of data between independently executing processors. The algorithms are tested on a standard Lennard-Jones benchmark problem for system sizes ranging from 500 to 100,000,000 atoms on several parallel supercomputers--the nCUBE 2, Intel iPSC/860 and Paragon, and Cray T3D. Comparing the results to the fastest reported vectorized Cray Y-MP and C90 algorithm shows that the current generation of parallel machines is competitive with conventional vector supercomputers even for small problems. For large problems, the spatial algorithm achieves parallel efficiencies of 90% and a 1840-node Intel Paragon performs up to 165 faster than a single Cray C9O processor. Trade-offs between the three algorithms and guidelines for adapting them to more complex molecular dynamics simulations are also discussed.

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

Electronics and optoelectronics of two-dimensional transition metal dichalcogenides.

Abstract: Single-layer metal dichalcogenides are two-dimensional semiconductors that present strong potential for electronic and sensing applications complementary to that of graphene.