Author
Yanli Yang
Bio: Yanli Yang is an academic researcher from Carnegie Institution for Science. The author has contributed to research in topics: Transcription factor & Biology. The author has an hindex of 2, co-authored 2 publications receiving 1289 citations.
Papers
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TL;DR: The results demonstrate that BZR1 is a positive regulator of the BR signaling pathway that mediates both downstream BR responses and feedback regulation of BR biosynthesis.
925 citations
TL;DR: It is reported that BRs induce dephosphorylation and accumulation of BZR1 protein and that BR signaling causes BzR1 deph phosphorylated and accumulation by inhibiting BIN2 activity.
Abstract: Brassinosteroids (BRs) are a class of steroid hormones essential for normal growth and development in plants. BR signaling involves the cell-surface receptor BRI1, the glycogen synthase kinase-3-like kinase BIN2 as a negative regulator, and nuclear proteins BZR1 and BZR2/BES1 as positive regulators. The interactions among these components remain unclear. Here we report that BRs induce dephosphorylation and accumulation of BZR1 protein. Experiments using a proteasome inhibitor, MG132, suggest that phosphorylation of BZR1 increases its degradation by the proteasome machinery. BIN2 directly interacts with BZR1 in yeast two-hybrid assays, phosphorylates BZR1 in vitro, and negatively regulates BZR1 protein accumulation in vivo. These results strongly suggest that BIN2 phosphorylates BZR1 and targets it for degradation and that BR signaling causes BZR1 dephosphorylation and accumulation by inhibiting BIN2 activity.
591 citations
TL;DR: The molecular basis of cytokinin and auxin interactions in response to environmental stresses is revealed, and light is shed on the improvement of drought tolerance without a growth penalty in tree by molecular breeding.
Abstract: Drought is a critical environmental factor that constrains plant survival and growth. Genetic engineering provides a credible strategy to improve the drought resistance of plants. Here, we generated transgenic poplar lines expressing the isopentenyl transferase gene (IPT) under the driver of PtRD26 promoter (PtRD26pro -IPT). PtRD26 is a senescence and drought inducible NAC transcription factor. PtRD26pro -IPT plants displayed multiple phenotypes, including improved growth and drought tolerance. Transcriptome analysis revealed that auxin biosynthesis pathway was activated in the PtRD26pro -IPT plants, leading to an increase in auxin contents. Biochemical analysis revealed that ARABIDOPSIS RESPONSE REGULATOR10 (PtARR10), one of the type-B ARR transcription factor in cytokinin pathway, was induced in PtRD26pro -IPT plants and directly regulated the transcripts of YUCCA4 (PtYUC4) and YUCCA5 (PtYUC5), two enzymes in auxin biosynthesis pathway. Overexpression of PtYUC4 enhanced drought tolerance, while simultaneous silencing of PtYUC4/5 evidently attenuated the drought tolerance of PtRD26pro -IPT plants. Intriguingly, PtYUC4/5 displayed a conserved thioredoxin reductase activity that is required for drought tolerance by deterring reactive oxygen species (ROS) accumulation. Our work reveals the molecular basis of cytokinin and auxin interactions in response to environmental stresses, and shed light on the improvement of drought tolerance without a growth penalty in tree by molecular breeding. This article is protected by copyright. All rights reserved.
6 citations
TL;DR: In this article , a TGACG-binding (TGA) transcription factor, PeTGA1, was isolated from Populus euphratica and was localized to the nucleus.
4 citations
TL;DR: In this paper , the role of PeHSFA2, a crucial gene in plant heat shock response from Populus euphratica, in regulating plant heat stress protection was investigated.
2 citations
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TL;DR: Results indicate BAK1 is a component of BR signaling, and Expression of a dominant-negative mutant allele of B AK1 causes a severe dwarf phenotype, resembling the phenotype of null bri1 alleles.
1,275 citations
TL;DR: Current understanding of the Ub/26S proteasome pathway in plants is described at the biochemical, genomic, and genetic levels, using Arabidopsis thaliana as the model, showing that this pathway is one of the most elaborate regulatory mechanisms in plants.
Abstract: Much of plant physiology, growth, and development is controlled by the selective removal of short-lived regulatory proteins. One important proteolytic pathway involves the small protein ubiquitin (Ub) and the 26S proteasome, a 2-MDa protease complex. In this pathway, Ub is attached to proteins destined for degradation; the resulting Ub-protein conjugates are then recognized and catabolized by the 26S proteasome. This review describes our current understanding of the pathway in plants at the biochemical, genomic, and genetic levels, using Arabidopsis thaliana as the model. Collectively, these analyses show that the Ub/26S proteasome pathway is one of the most elaborate regulatory mechanisms in plants. The genome of Arabidopsis encodes more than 1400 (or >5% of the proteome) pathway components that can be connected to almost all aspects of its biology. Most pathway components participate in the Ub-ligation reactions that choose with exquisite specificity which proteins should be ubiquitinated. What remains to be determined is the identity of the targets, which may number in the thousands in plants.
1,227 citations
TL;DR: BES1, a semidominant suppressor of bri1, is identified, which exhibits constitutive BR response phenotypes including long and bending petioles, curly leaves, accelerated senescence, and constitutive expression of BR-response genes.
1,064 citations
TL;DR: Evidence supporting the growth-defense tradeoff concept is addressed, as well as known interactions between defense signaling and growth signaling, which should provide a foundation for the development of breeding strategies to maximize crop yield to meet rising global food and biofuel demands.
1,035 citations
TL;DR: It is reported that EIN3 protein levels rapidly increase in response to ethylene and this response requires several ethylene-signaling pathway components including the ethylene receptors (ETR1 and EIN4), CTR1, Ein2, EIN5, and Ein6, and the ubiquitin/proteasome pathway.
831 citations