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Yi-Shao Liu

Bio: Yi-Shao Liu is an academic researcher from TSMC. The author has contributed to research in topics: Field-effect transistor & Layer (electronics). The author has an hindex of 12, co-authored 24 publications receiving 540 citations. Previous affiliations of Yi-Shao Liu include Purdue University & University of Illinois at Urbana–Champaign.

Papers
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Patent
29 Sep 2009
TL;DR: In this paper, field effect chemical sensor devices are used for chemical and/or biochemical sensing, and methods for single molecule detection are described. But they are not useful for amplification of target molecules by PCR.
Abstract: In one aspect, described herein are field effect chemical sensor devices useful for chemical and/or biochemical sensing. Also provided herein are methods for single molecule detection. In another aspect, described herein are methods useful for amplification of target molecules by PCR.

79 citations

Journal ArticleDOI
TL;DR: Application of biotinylated Hsp60 as a capture molecule for living (viable) L. monocytogenes in a microfluidic environment shows that HSp60 could be used for specific detection of L.monocytgenes on a biochip sensor platform.
Abstract: Efficient capture of target analyte on biosensor platforms is a prerequisite for reliable and specific detection of pathogenic microorganisms in a microfluidic chip. Antibodies have been widely used as ligands; however, because of their occasional unsatisfactory performance, a search for alternative receptors is underway. Heat shock protein 60 (Hsp60), a eukaryotic mitochondrial chaperon protein is a receptor for Listeria adhesion protein (LAP) during Listeria monocytogenes infection. This paper reports application of biotinylated Hsp60 as a capture molecule for living (viable) L. monocytogenes in a microfluidic environment. Hsp60, immobilized on the surface of streptavidin-coated silicon dioxide exhibited specific capture of pathogenic Listeria against a background of other Listeria species, Salmonella, Escherichia, Bacillus, Pseudomonas, Serratia, Hafnia, Enterobacter, Citrobacter, and Lactobacillus. The capture efficiency of L. monocytogenes was 83 times greater than another Listeria receptor, the mono...

73 citations

Journal ArticleDOI
TL;DR: A new impedance-based method to detect viable spores by electrically detecting their germination in real time within microfluidic biochips is presented, and it is believed that this is the first demonstration of this application in micro fluidic and BioMEMS devices.
Abstract: In this paper, we present a new impedance-based method to detect viable spores by electrically detecting their germination in real time within microfluidic biochips. We used Bacillus anthracis Sterne spores as the model organism. During germination, the spores release polar and ionic chemicals, such as dipicolinic acid (DPA), calcium ions, phosphate ions, and amino acids, which correspondingly increase the electrical conductivity of the medium in which the spores are suspended. We first present macro-scale measurements demonstrating that the germination of spores can be electrically detected at a concentration of 109 spores ml−1 in sample volumes of 5 ml, by monitoring changes in the solution conductivity. Germination was induced by introducing an optimized germinant solution consisting of 10 mM L-alanine and 2 mM inosine. We then translated these results to a micro-fluidic biochip, which was a three-layer device: one layer of polydimethylsiloxane (PDMS) with valves, a second layer of PDMS with micro-fluidic channels and chambers, and the third layer with metal electrodes deposited on a pyrex substrate. Dielectrophoresis (DEP) was used to trap and concentrate the spores at the electrodes with greater than 90% efficiency, at a solution flow rate of 0.2 μl min−1 with concentration factors between 107–109 spores ml−1, from sample volumes of 1–5 μl. The spores were captured by DEP in deionized water within 1 min (total volume used ranged from 0.02 μl to 0.2 μl), and then germinant solution was introduced to the flow stream. The detection sensitivity was demonstrated to be as low as about a hundred spores in 0.1 nl, which is equivalent to a macroscale detection limit of approximately 109 spores ml−1. We believe that this is the first demonstration of this application in microfluidic and BioMEMS devices.

58 citations

Patent
Chun-Wen Cheng1, Yi-Shao Liu1, Fei-Lung Lai1, Lin Wei-Cheng1, Ta-Chuan Liao1, Chien-Kuo Yang1 
30 May 2013
TL;DR: In this paper, a bio-field effect transistor (BioFET) and a method of fabricating a BioFET device are described using one or more process steps compatible with or typical to a complementary metal-oxide-semiconductor (CMOS) process.
Abstract: The present disclosure provides a bio-field effect transistor (BioFET) and a method of fabricating a BioFET device. The method includes forming a BioFET using one or more process steps compatible with or typical to a complementary metal-oxide-semiconductor (CMOS) process. The BioFET device includes a substrate, a transistor structure having a treated layer adjacent to the channel region, an isolation layer, and a dielectric layer in an opening of the isolation layer on the treated layer. The dielectric layer and the treated layer are disposed on opposite side of the transistor from a gate structure. The treated layer may be a lightly doped channel layer or a depleted layer.

56 citations

Patent
Yi-Shao Liu1, Chun-Ren Cheng1, Ching-Ray Chen1, Yi-Hsien Chang1, Fei-Lung Lai1, Cheng Chun-Wen1 
05 Dec 2012
TL;DR: In this article, a bio-field effect transistor (BioFET) device and methods of fabricating a BioFET and a bioFET device are presented, using one or more process steps compatible with or typical to a complementary metaloxide-semiconductor (CMOS) process.
Abstract: The present disclosure provides a bio-field effect transistor (BioFET) device and methods of fabricating a BioFET and a BioFET device. The method includes forming a BioFET using one or more process steps compatible with or typical to a complementary metal-oxide-semiconductor (CMOS) process. The BioFET device includes a gate structure disposed on a first surface of a substrate and an interface layer formed on a second surface of the substrate. The substrate is thinned from the second surface to expose a channel region before forming the interface layer.

55 citations


Cited by
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28 Jul 2005
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1(PfPMP1)与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员,通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

18,940 citations

01 May 2005

2,648 citations

Patent
02 Feb 2011
TL;DR: In this article, a flow expansion chamber is configured to allow fluids to flow from the expansion chamber to the outlet portion and to allow the fluids to interact along the way with material in the array of wells.
Abstract: An apparatus may include a semiconductor chip and a fluidics assembly. The semiconductor chip has an array of wells and an array of sensors and each sensor of the array of sensors is in fluid communication with a well of the array of wells. The fluidics assembly is located on top of the semiconductor chip and is configured to deliver fluids to the semiconductor chip. The fluidics assembly includes a flow expansion chamber configured to introduce the fluids, an outlet portion configured to pipe out the fluids, and a flow chamber portion. The flow chamber portion is configured to allow the fluids to flow from the flow expansion chamber to the outlet portion and to allow the fluids to interact along the way with material in the array of wells. The flow expansion chamber has a curved wall at the top or bottom so that the height of the flow expansion chamber at the center is less than at the walls that restrict the fluids to the left and right.

855 citations

Patent
27 May 2010
TL;DR: In this article, the authors present methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions.
Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

649 citations

Journal ArticleDOI
TL;DR: Technology 4403 Microfabrication 4403 Assembly and Interfacing 4404 Optical Integration 4405 Flow Control 4405 Standard Operations 4406 Sample Preparation 4406 Injection and Separation 4407 Fluidic Reactors 4407 Particle and Cell Sorting 4409 Cell Trapping and Culture 4409 Applications.
Abstract: Technology 4403 Microfabrication 4403 Assembly and Interfacing 4404 Optical Integration 4405 Flow Control 4405 Standard Operations 4406 Sample Preparation 4406 Injection and Separation 4407 Fluidic Reactors 4407 Particle and Cell Sorting 4409 Cell Trapping and Culture 4409 Applications 4410 Clinical Diagnostics 4410 Nucleic Acids 4411 Proteins 4412 Cell and Tissue Studies 4413 Environmental Monitoring 4414 Literature Cited 4414

427 citations