Yi-Sheng Chan

Bio: Yi-Sheng Chan is an academic researcher from Memorial Hospital of South Bend. The author has contributed to research in topics: Medicine & Arthroscopy. The author has an hindex of 26, co-authored 76 publications receiving 1693 citations. Previous affiliations of Yi-Sheng Chan include Chang Gung University.

Extracorporeal Shockwave for Chronic Patellar Tendinopathy

Abstract: BackgroundChronic patellar tendinopathy is an overuse syndrome with pathologic changes similar to tendinopathies of the shoulder, elbow, and heel. Extracorporeal shockwave was shown effective in many tendinopathies.HypothesisExtracorporeal shockwave therapy may be more effective than conservative treatment for chronic patellar tendinopathy.Study DesignRandomized controlled clinical trial; Level of evidence, 2.MethodsThis study consisted of 27 patients (30 knees) in the study group and 23 patients (24 knees) in the control group. In the study group, patients were treated with 1500 impulses of extracorporeal shockwave at 14 KV (equivalent to 0.18 mJ/mm 2 energy flux density) to the affected knee at a single session. Patients in the control group were treated with conservative treatments including nonsteroidal anti-inflammatory drugs, physiotherapy, exercise program, and the use of a knee strap. The evaluation parameters included pain score, Victorian Institute of Sports Assessment score, and ultrasonographi...

Arthroscopic evaluation of soft tissue injuries in tibial plateau fractures : Retrospective analysis of 98 cases

Abstract: Purpose: This investigation arthroscopically assesses the frequency of soft tissue injury in tibial plateau fracture according to the severity of fracture patterns. We hypothesized that use of arthroscopy to evaluate soft tissue injury in tibial plateau fractures would reveal a greater number of associated injuries than have previously been reported. Methods: From March 1996 to December 2003, 98 patients with closed tibial plateau fractures were treated with arthroscopically assisted reduction and osteosynthesis, with precise diagnosis and management of associated soft tissue injuries. Arthroscopic findings for associated soft tissue injuries were recorded, and the relationship between fracture type and soft tissue injury was then analyzed. Results: The frequency of associated soft tissue injury in this series was 71% (70 of 98). The menisci were injured in 57% of subjects (56 in 98), the anterior cruciate ligament (ACL) in 25% (24 of 98), the posterior cruciate ligament (PCL) in 5% (5 of 98), the lateral collateral ligament (LCL) in 3% (3 of 98), the medial collateral ligament (MCL) in 3% (3 of 98), and the peroneal nerve in 1% (1 of 98); none of the 98 patients exhibited injury to the arteries. No significant association was noted between fracture type and incidence of meniscus, PCL, LCL, MCL, artery, and nerve injury. However, significantly higher injury rates for the ACL were observed in type IV and VI fractures. Conclusions: Soft tissue injury was associated with all types of tibial plateau fracture. Menisci (peripheral tear) and ACL (bony avulsion) were the most commonly injured sites. A variety of soft tissue injuries are common with tibial plateau fracture; these can be diagnosed with the use of an arthroscope. Level of Evidence: Level III, diagnostic study.

Arthroscopic single- versus double-bundle posterior cruciate ligament reconstructions using hamstring autograft

Abstract: Summary This prospective study compared the clinical results of single- and doublebundle posterior cruciate ligament (PCL) reconstruction with a minimum follow-up of 2 years. There were 35 patients including 19 single- and 16 double-bundle posterior cruciate ligament reconstructions using hamstring autograft. The average age was 29:4 � 13:6 years versus 28:2 � 10:4 years; and the average follow-up was 41:0 � 13:1 months versus 28:2 � 4:2 months for single- and double-bundle reconstruction, respectively. The indication for surgery was functional disability of the knee due to pain and instability as the result of high-energy PCL injury. The evaluation parameters included functional assessment, ligament laxity, functional score and radiographs of the knee. The results showed no significant difference in functional assessment, ligament laxity, functional score and radiographic changes of the knee between the two techniques. The rate of overall satisfaction with the operation was comparable from patient and surgeon perspectives. Contrary to many recent reports, the results of this study showed that single- and double-bundle PCL reconstruction using hamstring autograft produced comparable clinical results in medium-term follow-up. The difference between singleand double-bundle PCL reconstruction, if any, can be concluded only with long-term results and larger number of patients. 2003 Elsevier Ltd. All rights reserved.

Arthroscopic suture fixation of tibial eminence avulsion fractures.

Abstract: Purpose This study presents the clinical results of a procedure for treating tibial eminence fractures of the anterior cruciate ligament (ACL) using arthroscopic reduction and No. 5 Ethibond sutures (Ethicon, Somerville, NJ). Methods This prospective study analyzed 36 patients who underwent arthroscopic reduction and suture fixation for image-proven ACL avulsion fractures of the tibial eminence. The classification of Meyers and McKeever identified 6 type II, 16 type III, and 14 type IV fractures. The mean follow-up period was 34.4 months (range, 24 to 91 months). Follow-up assessment included Lysholm knee score, Tegner activity score, International Knee Documentation Committee (IKDC) score, and KT-1000 arthrometer (MEDmetric, San Diego, CA) and radiographic evaluation. Results The mean preoperative Lysholm score in the 36 knees was 38 (range, 28 to 54); the mean postoperative Lysholm score was 98 (range, 83 to 100). The mean preinjury and preoperative Tegner scores in the 36 knees were 7.5 ± 1.5 (range, 5 to 9) and 3 ± 1.7 (range, 2 to 5), respectively. The mean postoperative Tegner score was 7.3 ± 1.7 (range, 5 to 9). At final follow-up, 34 patients (94.5%) were classified by IKDC score as normal or nearly normal (grade A or B). The IKDC classification was abnormal (grade C) in 2 patients (5.5%). All 36 fractures achieved union within 3 months. No significant complications, such as arthrofibrosis, loss of initial fixation, or wound infection, were noted. Conclusions Treating ACL avulsion fracture by arthroscopic suture fixation by use of 4 No. 5 Ethibond sutures can restore ACL length, stabilize fragments, promote early motion, and minimize morbidity. Level of Evidence Level IV, therapeutic case series.

Arthroscopy-assisted surgery for tibial plateau fractures: 2- to 10-year follow-up results.

Abstract: Purpose: This study evaluated the outcome of arthroscopy-assisted reduction with internal fixation for treating tibial plateau fractures at 2- to 10-year follow-up. Methods: Fifty-four patients with tibial plateau fractures treated by arthroscopy-assisted reduction with internal fixation were enrolled in this prospective study. According to the Schatzker classification, the fractures types were as follows: type I, 1 (2%); type II, 21 (39%); type III, 4 (7%); type IV, 10 (19%); type V, 8 (15%); and type VI, 10 (19%). The mean age at operation was 48 years (range, 22 to 68 years). The mean follow-up period was 87 months (range, 28 to 128 months). Clinical and radiologic outcomes were scored by the Rasmussen system. Results: The mean postoperative Rasmussen clinical score was 28.4 (range, 19 to 30), and the mean radiologic score was 16.1 (range, 12 to 18). Good or excellent clinical and radiologic results were achieved in 96% of patients. The 6 fracture types did not significantly differ with regard to Rasmussen score or rate of satisfactory results ( P > .05). Secondary osteoarthritis was noted in 10 injured knees (19%). All 54 fractures were successfully united. The mean preoperative fracture depression was 13.7 mm (range, 6 to 25 mm). Fracture depression at the final follow-up averaged 0.3 mm (range, 0 to 4 mm). No complications directly associated with arthroscopy were noted in any of the 54 patients. Conclusions: Arthroscopic surgery for tibial plateau fractures with associated soft-tissue injuries is a safe, reproducible, and effective procedure that provides precise diagnosis and effective treatment in a 1-stage procedure. Level of Evidence: Level IV, therapeutic case series.

Is It Time to Start Transitioning From 2D to 3D Cell Culture

Abstract: Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D) cell culturing techniques suggest compelling evidence that much more advanced experiments can be performed yielding valuable insights. When performing 3D cell culture experiments, the cell environment can be manipulated to mimic that of a cell in vivo and provide more accurate data about cell-to-cell interactions, tumor characteristics, drug discovery, metabolic profiling, stem cell research, and other types of diseases. Scaffold based techniques such as hydrogel-based support, polymeric hard material-based support, hydrophilic glass fiber, and organoids are employed, and each provide their own advantages and applications. Likewise, there are also scaffold free techniques used such as hanging drop microplates, magnetic levitation, and spheroid microplates with ultra-low attachment coating. 3D cell culture has the potential to provide alternative ways to study organ behavior via the use of organoids and is expected to eventually bridge the gap between 2D cell culture and animal models. The present review compares 2D cell culture to 3D cell culture, provides the details surrounding the different 3D culture techniques, as well as focuses on the present and future applications of 3D cell culture.

ALGINATE LYASE: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications.

Abstract: ▪ Abstract Alginate lyases, characterized as either mannuronate (EC 4223) or guluronate lyases (EC 42211), catalyze the degradation of alginate, a complex copolymer of α-L-guluronate and its C5 epimer β-D-mannuronate Lyases have been isolated from a wide range of organisms, including algae, marine invertebrates, and marine and terrestrial microorganisms This review catalogs the major characteristics of these lyases, the methods for analyzing these enzymes, as well as their biological roles Analysis of primary sequence data identifies some markedly conserved motifs that should help elucidate functional domains Information about the three-dimensional structure of a mannuronate lyase from Sphingomonas sp, combined with various mutagenesis studies, has identified residues that are important for catalytic activity in several lyases Characterization of alginate lyases will enhance and expand the use of these enzymes to engineer novel alginate polymers for applications in various industrial, agricult

Ongoing Positive Effect of Platelet-Rich Plasma Versus Corticosteroid Injection in Lateral Epicondylitis: A Double-Blind Randomized Controlled Trial With 2-year Follow-up

Abstract: Background: Platelet-rich plasma (PRP) has been shown to be a general stimulation for repair and 1-year results showed promising success percentages. Purpose: This trial was undertaken to determine the effectiveness of PRP compared with corticosteroid injections in patients with chronic lateral epicondylitis with a 2-year follow-up. Study Design: Randomized controlled trial; Level of evidence, 1. Methods: The trial was conducted in 2 Dutch teaching hospitals. One hundred patients with chronic lateral epicondylitis were randomly assigned to a leukocyte-enriched PRP group (n = 51) or the corticosteroid group (n = 49). Randomization and allocation to the trial group were carried out by a central computer system. Patients received either a corticosteroid injection or an autologous platelet concentrate injection through a peppering needling technique. The primary analysis included visual analog scale (VAS) pain scores and Disabilities of the Arm, Shoulder and Hand (DASH) outcome scores. Results: The PRP group was more often successfully treated than the corticosteroid group (P\.0001). Success was defined as a reduction of 25% on VAS or DASH scores without a reintervention after 2 years. When baseline VAS and DASH scores were compared with the scores at 2-year follow-up, both groups significantly improved across time (intention-to-treat principle). However, the DASH scores of the corticosteroid group returned to baseline levels, while those of the PRP group significantly improved (as-treated principle). There were no complications related to the use of PRP. Conclusion: Treatment of patients with chronic lateral epicondylitis with PRP reduces pain and increases function significantly, exceeding the effect of corticosteroid injection even after a follow-up of 2 years. Future decisions for application of PRP for lateral epicondylitis should be confirmed by further follow-up from this trial and should take into account possible costs and harms as well as benefits.