Author
Ying Sun
Bio: Ying Sun is an academic researcher from Peking Union Medical College. The author has contributed to research in topics: Bisulfite & Malachite green. The author has an hindex of 3, co-authored 3 publications receiving 35 citations.
Topics: Bisulfite, Malachite green, Fluorescence
Papers
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TL;DR: In this article, a tetrahydro[5]helicene-malonitrile derivative was designed and synthesized for selective detection of bisulfite, which exhibited high response speed and selectivity toward HSO3−.
Abstract: A new fluorescent probe tetrahydro[5]helicene-malononitrile derivative was designed and synthesized for selective detection of bisulfite. This fluorescent probe exhibites high response speed and selectivity toward HSO3− in PBS solution over other coexisting species accompanied by a distinct fluorescence color change from yellow to blue, which can be visually identified. The probe shows excellent sensitivity to HSO3− and the detection limit is as low as 0.27 μM. Once reacted with HSO3−, the signal-to-background ratio of fluorescence intensity can reach a 45-folds enhancement and the quantum yield dramatically increases from 1.9% to 52.3%. The response mechanism proved to be a nucleophilic addition reaction of probe with HSO3−, which was confirmed by 1H NMR, Mass titration and theory calculation. Additionally, the preliminary cell imaging experiments demonstrated that the probe could be used for intracellular bisulfite detection.
24 citations
TL;DR: A series of novel fluorogenic dyes based on 3-indole-Malachite Green, MGs 5-7, have been developed that are dark in solution but highly fluorescent when bound to the cognate reporter, fluorogen-activating protein (FAP).
13 citations
TL;DR: The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system and two novel FRET probes for selective real-time ratiometric imaging of SSPA activity in live S.Aureus cells are reported.
8 citations
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TL;DR: A general overview was provided with the aim to highlight the typical examples of the HSO3-/SO32- fluorescent probes reported since 2010, especially those in the past five years and wish this review will give some help to the researchers in this field.
71 citations
TL;DR: A novel turn-on fluorescent probe for the selective sensing of nitroreductase (NTR) activity and its initial applications in rapid, real-time detection and identification of ESKAPE pathogens have been reported.
62 citations
01 Jun 2018
TL;DR: Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology and have been widely used for biological imaging as mentioned in this paper.
Abstract: Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology. Those probes that respond to enzymatic catalysis illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. Here, we review recent advances in enzyme-activated fluorogenic probes for biological imaging. We organize our survey by enzyme classification, with emphasis on fluorophore masking strategies, modes of enzymatic activation, and the breadth of current and future applications. Key challenges such as probe selectivity and spectroscopic requirements are described alongside therapeutic, diagnostic, and theranostic opportunities.
61 citations
TL;DR: This review summarizes multiple strategies that utilize nanoclusters for detection of pathogenic bacteria and their distinct optical and catalytic properties enables them as efficient biosensors.
Abstract: Antibiotic-resistant bacterial infections cause more than 700 000 deaths each year worldwide. Detection of bacteria is critical in limiting infection-based damage. Nanomaterials provide promising sensing platforms owing to their ability to access new interaction modalities. Nanoclusters feature sizes smaller than traditional nanomaterials, providing great sensitive ability for detecting analytes. The distinct optical and catalytic properties of nanoclusters combined with their biocompatibility enables them as efficient biosensors. In this review, we summarize multiple strategies that utilize nanoclusters for detection of pathogenic bacteria.
46 citations
TL;DR: In this article, a colorimetric and ratiometric fluorescent probe DTF for HSO3− was synthesized from natural and renewable nopinone, which can quantitatively detect HSO-3− with high selectivity, sensitivity and fast response time.
45 citations