Author
Ylva Blixt
Bio: Ylva Blixt is an academic researcher. The author has contributed to research in topics: Food spoilage & Yersinia enterocolitica. The author has an hindex of 6, co-authored 6 publications receiving 1193 citations.
Papers
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TL;DR: It is concluded that a multivariate approach based on spectra of chemical compounds, may be helpful in order to analyse spoilage, at least for spoilage caused by lactic acid bacteria.
845 citations
TL;DR: It was shown that changes in the pH and the concentrations of l-lactate and glucose-6-phosphate during storage were able to explain 68% of the variation observed in the rate of spoilage.
139 citations
TL;DR: Results indicate that selective enrichment followed by buoyant density gradient centrifugation provides a convenient and user-friendly sample preparation method prior to PCR that separates Yersinia enterocolitica from PCR inhibitors originating from enrichment media and pork.
109 citations
TL;DR: The level of spoilage of vacuum-packaged beef, as calculated using a model based on two Tagushi sensors, correlated well with the degree of spoilages determined by the sensory panel (r2 = 0.94), and the signal patterns obtained from the meat from the different slaughterhouses did not change over a period of 6 months.
107 citations
TL;DR: Four enrichment PCR protocols for detecting unlysed cells of pathogenic Yersinia enterocolitica were studied and the optimal point in time of sample withdrawal was found to be different for each protocol employed, and early detection was favoured by concentrating the target cells.
37 citations
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TL;DR: This research presents a meta-analysis of 126 existing and new technologies in the gas chromatography field, and some new technologies that are being developed, as well as suggestions for further studies.
Abstract: 2.2. New Approaches 707 2.2.1. Optical Sensor Systems 707 2.2.2. Mass Spectrometry 708 2.2.3. Ion Mobility Spectrometry 708 2.2.4. Gas Chromatography 709 2.2.5. Infrared Spectroscopy 709 2.2.6. Use of Substance-Class-Specific Sensors 709 2.3. Combined Technologies 710 3. Companies 710 4. Application Areas 710 4.1. Food and Beverage 712 4.2. Environmental Monitoring 715 4.3. Disease Diagnosis 716 5. Research and Development Trends 718 5.1. Sample Handling 719 5.2. Filters and Analyte Gas Separation 719 5.3. Data Evaluation 720 6. Conclusion 721 7. References 722
1,266 citations
TL;DR: An overview of different physicochemical instrumental techniques for direct and indirect identification of bacteria such as: infrared and fluorescence spectroscopy, flow cytometry, chromatography and chemiluminescence techniques as a basis for biosensor construction is presented.
926 citations
TL;DR: The widespread occurrence of N-acyl homoserine lactones (AHL) in stored and spoiling fresh foods is reported for the first time and the potential implications for spoilage and food preservation are discussed.
920 citations
TL;DR: A review of the most recent works in electronic noses used in the food industry is presented in this paper, focusing on the applications within food quality monitoring that is, meat, milk, fish, tea, coffee and wines.
574 citations
TL;DR: A simple PCR assay that is specific for Salmonella spp.
Abstract: As part of a major international project for the validation and standardization of PCR for detection of five major food-borne pathogens, four primer sets specific for Salmonella species were evaluated in-house for their analytical accuracy (selectivity and detection limit) in identifying 43 Salmonella spp. and 47 non-Salmonella strains. The most selective primer set was found to be 139-141 (K. Rahn, S. A. De Grandis, R. C. Clarke, S. A. McEwen, J. E. Galan, C. Ginocchio, R. Curtiss III, and C. L. Gyles, Mol. Cell. Probes 6:271-279, 1992), which targets the invA gene. An extended determination of selectivity by using 364 strains showed that the inclusivity was 99.6% and the exclusivity was 100% for the invA primer set. To indicate possible PCR inhibitors derived from the sample DNA, an internal amplification control (IAC), which was coamplified with the invA target gene, was constructed. In the presence of 300 DNA copies of the IAC, the detection probability for primer set 139-141 was found to be 100% when a cell suspension containing 104 CFU/ml was used as the template in the PCR (50 CFU per reaction). The primer set was further validated in an international collaborative study that included 16 participating laboratories. Analysis with 28 coded (“blind”) DNA samples revealed an analytical accuracy of 98%. Thus, a simple PCR assay that is specific for Salmonella spp. and amplifies a chromosomal DNA fragment detected by gel electrophoresis was established through extensive validation and is proposed as an international standard. This study addresses the increasing demand of quality assurance laboratories for standard diagnostic methods and presents findings that can facilitate the international comparison and exchange of epidemiological data.
546 citations