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Yogendra H. Kanitkar
Researcher at Michigan State University
Publications - 6
Citations - 68
Yogendra H. Kanitkar is an academic researcher from Michigan State University. The author has contributed to research in topics: Loop-mediated isothermal amplification & Dehalococcoides. The author has an hindex of 5, co-authored 6 publications receiving 57 citations. Previous affiliations of Yogendra H. Kanitkar include Clemson University.
Papers
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Journal ArticleDOI
Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9
Yogendra H. Kanitkar,Robert D. Stedtfeld,Robert J. Steffan,Syed A. Hashsham,Alison M. Cupples +4 more
TL;DR: Loop-mediated isothermal amplification (LAMP) was developed as an alternative approach for the quantification of these genes, allowing the method to be performed using less-expensive and potentially field-deployable detection devices and quantification results were similar over a large range of gene concentrations.
Journal ArticleDOI
Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater.
Robert D. Stedtfeld,Tiffany M. Stedtfeld,Farag A. Samhan,Yogendra H. Kanitkar,Paul B. Hatzinger,Alison M. Cupples,Syed A. Hashsham +6 more
TL;DR: Of the tested field-able concentrations methods, the direct filter amplification (DFA) method had the lowest coefficient of variation among Dehalobacter spiked groundwater samples and lowest threshold time indicating high capture efficiency and low inhibition.
Journal ArticleDOI
Abundance of Chlorinated Solvent and 1,4-Dioxane Degrading Microorganisms at Five Chlorinated Solvent Contaminated Sites Determined via Shotgun Sequencing.
Hongyu Dang,Yogendra H. Kanitkar,Robert D. Stedtfeld,Paul B. Hatzinger,Syed A. Hashsham,Alison M. Cupples +5 more
TL;DR: The work illustrates the importance of shotgun sequencing to provide a more complete picture of the functional abilities of microbial communities because the approach is advantageous over current methods because an unlimited number of functional genes can be quantified.
Journal ArticleDOI
Development and application of a rapid, user-friendly, and inexpensive method to detect Dehalococcoides sp. reductive dehalogenase genes from groundwater
Yogendra H. Kanitkar,Robert D. Stedtfeld,Paul B. Hatzinger,Syed A. Hashsham,Alison M. Cupples +4 more
TL;DR: An approach that requires only low-cost laboratory equipment (a bench top centrifuge and a water bath) and requires less time and resources compared to qPCR is described, which detects RDase genes without DNA extraction or a thermal cycler.
Journal ArticleDOI
Most probable number with visual based LAMP for the quantification of reductive dehalogenase genes in groundwater samples
Yogendra H. Kanitkar,Robert D. Stedtfeld,Paul B. Hatzinger,Syed A. Hashsham,Alison M. Cupples +4 more
TL;DR: Deoxyuridine triphosphate (dUTP) and uracil DNA glycosylase (UNG) were incorporated into the assay to reduce the probability of false positives and a strong correlation was observed between the two data sets.