Y
Yongxin Zhao
Researcher at Carnegie Mellon University
Publications - 55
Citations - 5036
Yongxin Zhao is an academic researcher from Carnegie Mellon University. The author has contributed to research in topics: Microscopy & Medicine. The author has an hindex of 21, co-authored 49 publications receiving 3941 citations. Previous affiliations of Yongxin Zhao include Sun Yat-sen University & University of Alberta.
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Journal ArticleDOI
An Expanded Palette of Genetically Encoded Ca2+ Indicators
Yongxin Zhao,Satoko Araki,Jiahui Wu,Takayuki Teramoto,Yu-Fen Chang,Masahiro Nakano,Ahmed S. Abdelfattah,Manabi Fujiwara,Takeshi Ishihara,Takeharu Nagai,Takeharu Nagai,Robert E. Campbell +11 more
TL;DR: This series enables improved single-color Ca2+ imaging in neurons and transgenic Caenorhabditis elegans and develops blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change.
Journal ArticleDOI
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Nathan C. Klapoetke,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Yong Ku Cho,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Edward S. Boyden,Robert E. Campbell,Adam E. Cohen +21 more
TL;DR: In this article, a coexpression vector, Optopatch, enabled cross-talk-free genetically targeted all-optical electrophysiology without the use of conventional electrodes.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Robert E. Campbell,Adam E. Cohen,Nathan C. Klapoetke,Yong Ku Cho,Edward S. Boyden +21 more
TL;DR: The Optopatch platform enables high-throughput, spatially resolved electrophysiology without the use of conventional electrodes and revealed homeostatic tuning of intrinsic excitability in human stem cell–derived neurons.
Journal ArticleDOI
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Paul W. Tillberg,Fei Chen,Kiryl D. Piatkevich,Yongxin Zhao,Chih-Chieh Yu,Brian P. English,Linyi Gao,Anthony J Martorell,Ho-Jun Suk,Fumiaki Yoshida,Fumiaki Yoshida,Ellen M DeGennaro,Douglas H. Roossien,Guanyu Gong,Uthpala Seneviratne,Steven R. Tannenbaum,Robert Desimone,Dawen Cai,Edward S. Boyden +18 more
TL;DR: ProExM as mentioned in this paper is a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins.
Journal ArticleDOI
Expansion microscopy: principles and uses in biological research.
TL;DR: This Perspective reviews available methods and provides practical guidance for users of Expansion microscopy, which allows super-resolution images of diverse samples to be acquired on conventional microscopes, thus democratizing super- resolution imaging.