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Yongyi Cui

Bio: Yongyi Cui is an academic researcher from Chungbuk National University. The author has contributed to research in topics: Doritaenopsis & Arabidopsis. The author has an hindex of 9, co-authored 17 publications receiving 276 citations.

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Journal ArticleDOI
TL;DR: The results of this experiment suggest that increased number of air exchanges of the culture vessel, decreased sucrose concentration, and positive DIF in combination with high PPF level enhanced growth and acclimatization of Rehmannia glutinosa plantlets.
Abstract: Rehmannia glutinosa plantlets were cultured for 4 weeks under different culture conditions to determine the optimum environment for in vitro growth and ex vitro survival. Plantlet growth increased with an increasing number of air exchanges of the culture vessel, exhibiting greatest shoot weight, total fresh weight, leaf area, and chlorophyll content at 4.4 h−1 of air exchanges. High sucrose concentration (30 g l−1) increased root weight but reduced shoot growth. Net photosynthetic rates of the plantlets were greatest when sucrose was not added to the medium. On the other hand, ex vitro survival of the plantlets was not influenced by sucrose concentration. In the experiment on difference in photoperiod and dark period temperatures (DIF) and photosynthetic photon flux (PPF), plantlet growth increased as DIF and PPF levels increased. Particularly, increasing PPF level had a more distinctive effect on plantlet growth than increasing DIF level. The interaction of DIF × PPF was also significant, showing the greatest plantlet growth in positive DIF (+8 DIF) and a high PPF (210 μmol m−2 s−1). In conclusion, the results of this experiment suggest that increased number of air exchanges of the culture vessel, decreased sucrose concentration, and positive DIF in combination with high PPF level enhanced growth and acclimatization of Rehmannia glutinosa plantlets.

69 citations

Journal ArticleDOI
TL;DR: The results strongly suggest that the control of transpiration during early stage after transplantation plays a key role in the acclimatization process, and photoautotrophic conditions could be a solution to solve the problems associated with transplantation stress.
Abstract: The photosynthetic responses of Rehmannia glutinosa grown under photoautotrophic or heterotrophic conditions in vitro were investigated after transfer to greenhouse conditions. In addition, the changes in carbohydrate content and survival rates of the plantlets were evaluated. During six days after transplantation, the photosynthetic rate declined and photoinhibitory impairments represented by decrease of Fv/Fm and chlorophyll content were observed regardless of environmental conditions in vitro. Excessive transpiration was observed in plantlets grown under heterotrophic conditions during that period. Fructose and glucose content of the plantlets grown under photoautotrophic conditions increased with time and reached almost the same level of field grown plants after day 15. Under heterotrophic conditions, in contrast, the content of these sugars decreased continuously during that period. It is suggested that high survival rate of plantlets grown under photoautotrophic conditions has to be attributed to improvement of photosynthetic competence by imposed high light intensity and CO2 concentration in vitro. The results strongly suggest that the control of transpiration during early stage after transplantation plays a key role in the acclimatization process, and photoautotrophic conditions could be a solution to solve the problems associated with transplantation stress.

51 citations

Journal ArticleDOI
TL;DR: The results of the current study are useful for scale-up process for the production of bioactive compounds from D. candidum protocorm suspension cultures.

36 citations

Journal ArticleDOI
TL;DR: Drought effects on photosynthesis, stomatal conductance, transpiration, reducing sugar, total sugar, starch, protein, phosphoenolpyruvate carboxylase, superoxide dismutase, and peroxidase activity were studied on Doritaenopsis.

31 citations

Journal ArticleDOI
TL;DR: Using differential gene expression, it is shown that the leaves of a hybrid of Phalaenopsis species, Doritaenopsis ‘Tinny Tender’, undergo major metabolic modifications that result in the production of sucrose and amino acids, both of which can sustain bud outgrowth, and auxin and ethylene, which may play important roles in awaking the dormant buds.
Abstract: Massive flowering of tropical Phalaenopsis orchids is coordinated by the cold-induced release of reproductive bud dormancy. Light and temperature are the two key factors integrated by the dormancy mechanism to both stop and reactivate the meristem development of many other angiosperm species, including fruit trees and ornamental plants. It is well established that leaves and roots play a major role in inducing flower development; however, currently, knowledge of molecular events associated with reproductive bud dormancy release in organs other than the bud is limited. Using differential gene expression, we have shown that the leaves of a hybrid of Phalaenopsis species, Doritaenopsis ‘Tinny Tender’, undergo major metabolic modifications. These changes result in the production of sucrose and amino acids, both of which can sustain bud outgrowth, and auxin and ethylene, which may play important roles in awaking the dormant buds. Intake of abscisic acid and synthesis of the hormone jasmonate may also explain the inhibition of vegetative growth that coincides with bud growth. Interestingly, many genes that were upregulated by cold treatment are homologous for genes involved in flower induction and vernalization in Arabidopsis, indicating that processes regulating flowering induction and those regulating reproductive bud dormancy release may use similar pathways and effector molecules.

20 citations


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Journal ArticleDOI
TL;DR: R and RFr resulted in the highest stem elongation but also resulted in stem fragility due to excessive elongation of the third internode, which may be required to adjust the ratio of red light when mixed with blue or florescent.

384 citations

Journal ArticleDOI
TL;DR: It is indicated that the photoautotrophic growth in vitro of many plant species can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the Vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar.
Abstract: Research has revealed that most chlorophyllous explants/plants in vitro have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is not due to poor photosynthetic ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Moreover, numerous studies have been conducted on improving the in vitro environment and investigating its effects on growth and development of cultures/plantlets on nearly 50 species since the concept of photoautotrophic micropropagation was developed more than two decades ago. These studies indicate that the photoautotrophic growth in vitro of many plant species can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. This paper reviews the development and characteristics of photoautotrophic micropropagation systems and the effects of environmental conditions on the growth and development of the plantlets. The commercial applications and the perspective of photoautotrophic micropropagation systems are discussed.

156 citations

Journal ArticleDOI
TL;DR: This review proposes the adoption of the term ‘priming’ for tissue culture propagation and outlines the approaches to in vitro propagule priming, based on the changes to the growth enviroment prior to and/or upon transplanting.
Abstract: Production of high-quality, vigorous tissue-culture-derived propagules requiles efficient ways for the enhancement of their post-transplanting ability for water management, photosynthesis, and resistance to diseases. Certain molecules, environmental factors, microorganisms, or their parts, can pre-sensitize cellular metabolism of plants, so upon exposure to stress these pre-sensitized, or ‘primed’, plants are able to respond quicker, and to a higher degree than nonprimed, and thus cope better with the challenge. In this review we propose the adoption of the term ‘priming’ for tissue culture propagation and outline the approaches to in vitro propagule priming, based on the changes to the growth enviroment (chemical, physical, and biological) prior to and/or upon transplanting. Major emphasis has been placed on in vitro and ex vitro biopriming (priming with beneficial microorganisms).

108 citations

Journal ArticleDOI
01 Aug 2007
TL;DR: Acclimation can be speeded up by hardening of plantlets in vitro or after transplantation by decreasing the transpiration rate by antitranspirants including abscisic acid, or by increasing photosynthetic rate by elevated CO2 concentration.
Abstract: Plantlets grown in vitro might be easily impaired by sudden changes in environmental conditions after ex vitro transfer. They usually need several weeks under shade and gradually decreasing air humidity to acclimate to the new conditions and to correct all abnormalities in their anatomy and physiology induced by special conditions of in vitro culture. For plant survival, the most important changes include development of cuticle, epicuticular waxes, and effective stomatal regulation of transpiration leading to stabilization of water status. For plant growth, changes in photosynthetic parameters (chlorophyll content, chloroplast ultrastructure, efficiency of photosystem 2, net photosynthetic rate) ensuring fully autotrophic growth with the rate corresponding to naturally grown plants are the most important. Acclimation can be speeded up by hardening of plantlets in vitro or after transplantation by decreasing the transpiration rate by antitranspirants including abscisic acid, or by increasing photosynthetic rate by elevated CO2 concentration.

82 citations