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Yulita Nurchayati

Bio: Yulita Nurchayati is an academic researcher from Diponegoro University. The author has contributed to research in topics: Callus & Horticulture. The author has an hindex of 5, co-authored 22 publications receiving 90 citations.

Papers
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DOI
01 Mar 2010
TL;DR: It is concluded that Ipomoea aquatica and Nymphaea sp.
Abstract: Aquatic plants have important role in water ecosystem. They serve as the main producer, oxygen suplier and heavy metal absorbtion. Beside that, aquatic plants also economically potencial, such as sources of chlorophyll, carotenoid and vitamin C. The aim of this experiment is to analize the content of total chlorophyll, carotenoids and vitamin C in 13 species of aquatic plants. It is expected that these plants maybe used for commercial purposes. The plants were collected from Rawa Pening, Genuk stream, and brackish water shrim pond in Kendal and Jepara. These chemical analysis were done by spectrophotometer, whereas vitamin C content was measured using iodometric titration method. Results indicated that the highest content of chlorophyl was resulted by Ipomoea aquatica, which is 22,1 mg/L. The highest content of carotenodid and vitamin C is resulted by Nymphaea sp., which were 3,42 mg/L and 14,1 mg/30 g respectively. It is concluded that Ipomoea aquatica and Nymphaea sp. have good commercial value as sources of pigment and vitamin C.

33 citations

01 Jun 2009
TL;DR: The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves, however the lowest one was found in sweet basil, suggesting that papaya's leave could be a food supplement.
Abstract: Chlorophyll is plant pigment which consumed as food supplement Chlorophyll source consumed now is from alfalfa’s leaves besides algae such as Spirulina and Chlorella Chlorophyll content find in all kinds of plant especially vegetables so that have potent to be source of chlorophyll The aims of this research are first to explore another chlorophyll source beside Leguminosae, from vegetables such as sweet basil, spinach, water spinach, cassava leaves, pegagan, and papaya’s leaves Second, to study the vegetables potent to be food supplement The experiment has been conducted by extracting 0,1 g each leaves in 10 mL acetone 85% The extract was fitered with Whatman paper No 1, thus record the absorbance by using spektrofotometer UV Vis Statistically analysis used to analyze chlorophyll content is Analysis of Variance (ANOVA) with Randomized Complete Design (RCD) in 95% significancy If the ANOVA showed significantly different, the analysis were continued with Duncan’s test The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves, however the lowest one was found in sweet basil Papaya’s leave could be a food supplement

19 citations

DOI
01 Jan 2010
TL;DR: The result showed that combination of BAP and NAA was able to induce callogenesis from hipocotyl of alfalfa and the optimal callus was obtained in combination of NAA 2 ppm and BAP 0 ppm.
Abstract: Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, and hepatitis. Propagation of this plant in Indonesia face a problem which has no embryo. One method to propagate this plant is by tissue culture or micropropagation. Callus induction is first step in micropropagation to produce callus which will be regenerated to become planlet. The aims of this research are to induce callogenesis from hipocotyl of alfalfa with addition Benzyl Amino Purine (BAP) and α Naphtalene Acetic Acid (NAA), and to determine the proper combination of BAP and NAA to produce the optimal callus. The experiment has been conducted by using 12 combination of BAP and NAA with Completely Randomized Design (CRD) in 4x3 factorial pattern by 5 replicates. Data were analyzed by ANOVA 95% Degrees of Freedom (DF). If there was significance result, it was followed by DMRT analyzed at 95 % DF. The result showed that combination of BAP and NAA was able to induce callogenesis from hipocotyl of alfalfa. The optimal callus was obtained in combination of BAP 0 ppm and NAA 2 ppm.

12 citations

DOI
01 Jan 2009
TL;DR: The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves, however the lowest one was found in sweet basil.
Abstract: Chlorophyll is a plant pigment which recently have been popular as food supplement. The source of chlorophyll consumed now are alfalfa’s leaves algae such as Spirulina and Chlorella.. Chlorophyll content find in all kinds of plant especially vegetables so that have potent to be source of chlorophyll. The aims of this research are to explore alternative source of chlorophyll aside of Leguminosae, from vegetables such as sweet basil, spinach, water spinach, cassava leaves, pegagan, and papaya’s leaves and to study the vegetables potent to be food supplement. The experiment has been conducted by extracting 0,1 g each leaves in 10 mL acetone 85%. The extract was filtered with Whatman paper No. 1, thus record the absorbance by using spektrofotometer UV Vis. Statistical analysis was used to analyze chlorophyll content is Analysis of Variance (ANOVA) with Randomized Complete Design (RCD) in 95% significancy. If the ANOVA showed significantly different, the analysis were continued with Duncan’s test. The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves. However the lowest one was found in sweet basil. Papaya’s leave potential as food supplement.

12 citations

Journal ArticleDOI
10 Oct 2020
TL;DR: In this article, the authors menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x4, dengan penelitian ini bertujuan untuk mengetahui and menganalisis jenis eksplan and konsentrasi TDZ ying tepat untuk maningkatkan respon viabilitas and organogenesis eksplans.
Abstract: Kentang (Solanum tuberosum L.) merupakan komoditas yang berpotensi dalam diversifikasi pangan, namun perbanyakan bibit dengan umbi tidak efektif untuk persediaan bibit nasional. Solusi dari permasalahan tersebut adalah perbanyakan dengan kultur jaringan melalui teknik organogenesis. Penelitian ini bertujuan untuk mengetahui dan menganalisis jenis eksplan dan konsentrasi TDZ yang tepat untuk meningkatkan respon viabilitas dan organogenesis eksplan. Metode yang digunakan adalah induksi organogenesis. Eksplan batang dipotong melintang dan diinisiasi dalam media MS dengan konsentrasi TDZ berbeda. Desain penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x4. Faktor pertama adalah jenis eksplan (batang tanpa tunas atau B0 dan batang bertunas atau B1), dan faktor kedua adalah konsentrasi TDZ (0ppm atau T0, 4,5ppm atau T4,5, 6ppm atau T6 dan 7,5ppm atau T7,5). Parameter yang diamati adalah persentase eksplan steril, persentase eksplan hidup, jumlah tunas, jumlah daun dan jumlah akar. Hasil penelitian menunjukkan bahwa jenis eksplan batang bertunas cenderung meningkatkan viabilitas dan persentase eksplan hidup daripada jenis eksplan batang tanpa tunas, sedangkan peningkatan konsentrasi TDZ cenderung menurunkan viabilitas dan persentase eksplan hidup. Jenis eksplan dan konsentrasi TDZ mempengaruhi secara nyata jumlah tunas, daun dan akar. Peningkatan konsentrasi TDZ sampai 4,5ppm merupakan konsentrasi optimal dalam meningkatkan jumlah tunas dan daun. Kata kunci : Solanum tuberosum, TDZ, jenis eksplan, , viabilitas, organogenesis

6 citations


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Journal ArticleDOI
13 Apr 2019
TL;DR: In this article, the results showed that aquadest extract can be categorized as the strong antioxidant and antiradical activity, ethyl acetat as a medium antioxidant activity.
Abstract: Exposure of sunlight lead tropical Sargassum sp. to maintain their growth and moreover to bring up their secondary metabollite for life struggling. Sargassum sp. has bioactive compounds that has a potential antioxidant acitivity such as phenolic compounds as well as chlorophyll and carotenoids. This research was conducted to determine antioxidant activities, phenolic compound and pigmens of Sargassum sp. with different solvent that have different polarities. Sample was macerate with n-heksana, ethyl acetate, methanol and aquadest. All the parameters were done spectrophotometrically. IC 50 was used to determine the antioxidant activity by antiradical scavenging activity using DPPH (515 nm). Total phenolic compound were tested by Folin-Ciocalteu solution and used gallic acid as standard (725 nm). The chlorophylls a content were measured at wavelength 662 nm and 645 nm and carotenoids were measured at wavelength 470 nm. The results showed best IC 50 is achieved by aquadest extract (72.95 ±0.22 ppm). The highest Total phenolic compound is achieved by ethyl acetat extract (120.29 ±0,404 mg GAE/g sample). The highest chlorophyll a content is achieved by ethyl acetat extract (18.23 ± 0,049 mg/g sample) and the highest carotenoid content is achieved by ethyl acetat extract extract (60.65 ±0,008 µmol/g sample). It can be concluded that aquadest extract can be categorized as the strong antioxidant and antiradical activity, ethyl acetat as a medium antioxidant activity. The simple and save methods of aquadest extract promising that Sargassum sp. frorn Indonesia is a good candidate compoud for nutraceutical and cosmeceutical approach.

25 citations

Dissertation
20 Sep 2019
TL;DR: High anthocyanin content in this plant is able to be a source of antioxidants to ward off free radicals, the need for this crop is quite high, but it is not being offset by the farmers' low yields.
Abstract: INDONESIA: Bayam Merah (Alternanthera amoena Voss. var. Red Leaf) merupakan tanaman dengan banyak manfaat bagi kesehatan, tingginya kandungan antosianin pada tanaman ini, mampu menjadi sumber antioksidan untuk menangkal radikal bebas. Kebutuhan akan tanaman ini cukup tinggi, namun tidak diimbangi oleh hasil produksi petani yang tergolong rendah. Oleh karena itu, perlu dilakukan upaya pemuliaan tanaman melalui induksi poliploidi menggunakan oryzalin, untuk menghasilkan tanaman dengan jumlah kromosom yang berlipat ganda, sel yang lebih besar, karakter morfologi dan produktivitas yang lebih baik. Penggunaan oryzalin jauh lebih efektif dalam poliploidi tanaman, dengan konsentrasi rendah (µM), dapat menghasilkan tanaman poliploidi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh lama perendaman dan kosentrasi oryzalin terhadap jumlah kromosom, stomata, karakter morfologi dan produktivitas tanaman A. amoena Voss. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) dua faktorial, faktor pertama yaitu lama perendaman oryzalin selama 4, 8 dan 24 jam, fakor kedua yaitu konsentrasi 0, 1.25, 2.5, 3.75 dan 5µM, dan dilakukan sebanyak 3 kali ulangan. Parameter yang diamati yaitu jumlah kromosom, karakter stomata, karakter morfologi dan produktivitas tanaman. Analisis data menggunkan ANOVA dengan uji lanjut Duncan 5%. Hasil penelitian menunjukkan bahwa, pemberian oryzalin mampu meningkatkan jumlah kromosom tanaman A. amoena Voss. varietas Red Leaf, yaitu menghasilkan kromosom 3n=18 (Triploid), 4n=24 (Tetraploid), 5n= 30 (Pentaploid) dan 6n= 36 (Hexaploid). Perlakuan yang paling optimal adalah lama perendaman 8 jam dengan konsentrasi 2,5 µM Mampu mempengaruhi ukuran stomata, jumlah kloroplas di sel penjaga, karakter morfologi dan produktivitas tanaman A. amoena Voss. varietas Red Leaf. ENGLISH: Red Spinach (Alternanthera amoena Voss. Var. Red Leaf) is a plant with a ton of health benefits, high anthocyanin content in this plant is able to be a source of antioxidants to ward off free radicals. The need for this crop is quite high, but it is not being offset by the farmers' low yields. Therefore, efforts should be made to plant breeding through induction of polyploidy using oryzalin, to produce plants with multiple chromosomes, larger cells, morphological characters and better productivity. The use of oryzalin is far more effective in plant polyploidy, because with only low concentrations (µM), it is already able to produce polyploidy plants. The purpose of this study was to determine the effect of the interaction of immersion duration and oryzalin concentration on increasing the genetic diversity of A. amoena Voss plants. This research method uses two factorial Randomized Block Design (RBD), which has the length immersion of oryzalin of 4, 8 and 24 hours with concentrations of 0, 1.25, 2.5, 3.75 and 5µM, and carried out 3 times. The results obtained were analysed using analysis of variance (ANOVA) with Duncan's further test 5%. The results showed that, administration of oryzalin can increase the number of A. amoena Voss chromosomes. Red Leaf varieties, which produce chromosomes 3n = 18 (Triploid), 4n = 24 (Tetraploid), 5n = 30 (Pentaploid) and 6n = 36 (Hexaploid). The most optimal treatment is immersion time of 8 hours with a concentration of 2.5 µM able to affect the size of the stomata, the amount of chloroplasts in guard cells, morphological characters and productivity of A. amoena Voss plants. Red Leaf varieties.

19 citations

Journal ArticleDOI
TL;DR: The use of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation.
Abstract: Introduction: Photodynamic inactivation has been developed to kill pathogenic microbes. In addition, some techniques have been introduced to minimize the biofilm resistance to antifungal properties in inhibiting cell growth. The principle of photodynamic inactivation different to antifungal drugs therapy which is resistant to biofilms. The presence of reactive oxygen species (ROS) that generating in photodynamic inactivation mechanisms can be damaging of biofilm cells and the principle of light transmission that could be penetrating in matrix layers of extracellular polymeric substance (EPS) until reaching the target cells at the base layers of biofilm. The present work aims to explore the potential of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser. The potential of chlorophyll photosensitizer was evaluated based on the efficacy of inactivation C. albicans biofilm cell through a cell viability test and an organic compound test. Methods: The treatment of photoinactivation was administered to 12 groups of C. albicans biofilm for four days using the 445 nm laser and the 650 nm laser. The 445 nm and 650 nm lasers activated the chlorophyll extract of the papaya leaf (0.5 mg/L) at the same energy density. The energy density variation was determined as 5, 10, 20, 30 and 40 J/cm2 with the duration of exposure of each laser adjusted to the absorbance percentage of chlorophyll extract of the papaya leaf. Results: The absorbance percentage of chlorophyll extracts of the papaya leaf on wavelengths of 650 nm and 445 nm respectively were 22.26% and 60.29%, respectively. The most effective treated group was a group of the laser with the addition of chlorophyll, done by the 650 nm lasers with inactivation about 32% (P=0.001), while the 445 nm lasers only 25% (P=0.061). The maximum malondialdehyde levels by treatment of the laser 650 nm were (0.046±0.004) nmol/mg. Conclusion: The use of chlorophyll extract of the papaya leaf as a photosensitizer, resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation up to 32% (with chlorophyll) and 25% (without chlorophyll). The utilization of chlorophyll extract of the papaya leaf would increase the antifungal effects with the activation by the diode laser in the biofilm of C. albicans.

15 citations

Journal ArticleDOI
TL;DR: The most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable.
Abstract: Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA ( α = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. Moreover, t he optimum treatment will be used to produce secondary metabolite and seed s y nthetic by cell suspension culture.

11 citations

Dissertation
16 Jan 2018
TL;DR: Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap (RAL), perkecambahan biji cendana mengg unakan IBA dan BAP, anda pengaruh 2,4-D terhadap induksi kalus embrionik dari daun dan kotiledon c endana (Santalum album L.) secara in vitro.
Abstract: INDONESIA: Cendana (Santalum album L.) merupakan tanaman yang tumbuh khusus di Nusa Tenggara Timur serta memiliki beragam manfaat yaitu minyaknya digunakan dalam dunia farmasi, kosmetik dan parfum, sedangkan kayunya digunakan sebagai patung, tasbih dan kipas. Kebutuhan cendana didunia sangatlah tinggi sehingga kekurangan cendana didunia mencapai 80ton pertahun. Kekurangan ini disebabkan karena tanaman cendana yang mudah mati sehingga budidaya secara konvensional sulit dilakukan. Kondisi ini mendorong untuk melakukan budidaya secara massal menggunakan teknik kultur in vitro. Kultur in vitro dilakukan melalui perkecambahan dan induksi kalus embrionik dari daun dan kotiledon dengan penambahan 2,4-D. Induksi kalus kotiledon harus dimulai dari proses perkecambahan dengan penambahan IBA, BAP dan GA3. Oleh karena itu penelitian ini bertujuan untuk mengetahui pengaruh IBA, BAP dan GA3 terhadap perkecambahan biji serta pengaruh 2,4-D terhadap induksi kalus embrionik dari daun dan kotiledon cendana (Santalum album L.) secara in vitro. Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap (RAL). Perkecambahan biji cendana menggunakan IBA dan BAP dengan konsentrasi 0 mg/L, 0.5 mg/L, 1 mg/L, 1.5mg/L, 2mg/L dan 2.5mg/L sedangkan GA3 0mg/L, 5mg/L, 10mg/L, 15mg/L, 20mg/L dan diulang masing-masing sebanyak 5 kali. Untuk induksi kalus embrionik daun dan kotiledon cendana menggunakan penambahan 2,4-D dengan konsentrasi 0mg/l, 1mg/L, 2mg/L, 3mg/L dan diulang masing-masing sebanyak 6 kali. Data perkecambahan biji dan kalus cendana dianalisis dengan uji Oneway Anova α= 5%. Apabila terdapat perbedaan signifikan maka dilanjutkan dengan uji Beda Nyata Jujur (BNJ) dengan taraf signifkan 5%. Hasil penelitian menunjukkan bahwa ada pengaruh pemberian IBA, BAP dan GA3 terhadap perkecambahan biji dan 2,4-D terhadap induksi kalus embriogenik daun dan kotiledon tanaman cedana. Konsentrasi yang paling terbaik dalam meningkatkan perkecambahan biji cendana adalah pada hari muncul kecambah GA3 20mg/L (7.6 HST); tinggi batang IBA 1.5mg/L (4.616cm); panjang akar IBA 1mg/L (5.25cm) dan jumlah daun GA3 10mg/L (4.8). Konsentrasi yang paling terbaik terhadap induksi kalus embrionik dengan penambahan 2,4-D yaitu hari munculnya kalus 3mg/L (19.666) pada daun dan 3mg/L (17.666) pada kotiledon; persentase pertumbuhan kalus 2mg/L (99.17%) pada daun dan 2mg/L (100%) pada kotiledon; berat basah kalus 2mg/l (0.180783gr) pada daun dan 1mg/L (0.9002) pada kotiledon. Untuk warna kalus yang dihasilkan adalah putih kekuningan, putih kehijauan, hijau dan hijau kekuningan, sedangkan teksturnya remah, kompak dan intermediet. ENGLISH: Sandalwood (Santalum album L.) is specially growed plant in Nusa Tenggara Timur. Its oil is widely used in pharmacy, cosmetics, and perfumery industry while its wood is used for statue, rosary, and fan. High demand for sandalwood resulted in shortage of sandalwood reached 80 tons per year. This is due to vulnerability of sandalwood to easily die so the conventional cultivation is difficult to be done. To overcome this, mass cultivation using in vitro technique is highly recommended. In vitro culture is done through germination and induction of embryonic callus from leaves and cotyledon by adding 2,4-D. Induction of cotyledon callus should starts from germination with the addition of IBA, BAP, and GA3. Therefore, this study aims to determine the influence of IBA, BAP and GA3 on seed germination as well as the effect of 2.4-D addition on embryonic callus induction from Sandalwood leaves and cotyledon in vitro. This experimental research was conducted using complete randomized design method. Germination of sandalwood seed was done using IBA and BAP with concentration varies from 0 mg/L, 0.5 mg/L, 1 mg/L, 1.5mg/L, 2mg/L and 2.5mg/L while GA3 0mg / L, 5mg / L, 10mg / L , 15mg / L, 20mg / L and repeated 5 times each. Callus embryonic induction of leaf and cotyledon were done by addition of 2,4-D with concentration 0mg / l, 1mg / L, 2mg / L, 3mg / L and repeated each of 6 times. Data of seed germination and callus of sandalwood were analyzed by Oneway Anova test α = 5%. If there is significant difference then continued with HSD test (BNJ) with 5% significance level. The results showed that there was an effect of adding IBA, BAP and GA3 on seed germination and 2,4-D on induction of embryogenic callus from sandalwood leaf and cotyledon. The best concentration for increasing the germination of sandalwood seeds is the sprouts appear in GA3 20mg/L (7.6 HST); stem height in 1.5mg/L IBA (4.616cm); length of root in IBA 1mg/L (5.25cm) and leaf amount in GA3 10mg/L (4.8). The best concentration on induction of embryogenic callus by the addition of 2,4-D on the day of callus emergence 3mg/L (19,666) on leaves and 3mg/L (17.666) on cotyledon; percent growth of callus 2mg/L (99.17%) on leaves and 2mg/L(100%) on cotyledon; wet weight callus 2mg /L (0.180783gr) on leaves and 1mg/L (0.9002gr) on cotyledon. For the resulting callus color is yellowish white, greenish green, green and yellowish green, while the texture is crumb, compact and intermediates.

10 citations