Yung-Chi Cheng

Bio: Yung-Chi Cheng is an academic researcher from Yale University. The author has contributed to research in topics: Medicine & DNA polymerase. The author has an hindex of 87, co-authored 683 publications receiving 42508 citations. Previous affiliations of Yung-Chi Cheng include University of Georgia & Vion Pharmaceuticals, Inc..

Antisense oligonucleotides as therapeutic agents--is the bullet really magical?

Abstract: Because of the specificity of Watson-Crick base pairing, attempts are now being made to use oligodeoxynucleotides (oligos) in the therapy of human disease. However, for a successful outcome, the oligo must meet at least six criteria: (i) the oligos can be synthesized easily and in bulk; (ii) the oligos must be stable in vivo; (iii) the oligos must be able to enter the target cell; (iv) the oligos must be retained by the target cell; (v) the oligos must be able to interact with their cellular targets; and (vi) the oligos should not interact in a non-sequence-specific manner with other macromolecules. Phosphorothioate oligos are examples of oligos that are being considered for clinical therapeutic trials and meet some, but not all, of these criteria. The potential use of phosphorothioate oligos as inhibitors of viral replication is highlighted.

A unified model for the flicker noise in metal-oxide-semiconductor field-effect transistors

Abstract: A unified flicker noise model which incorporates both the number fluctuation and the correlated surface mobility fluctuation mechanism is discussed. The latter is attributed to the Coulombic scattering effect of the fluctuating oxide charge. The model has a functional form resembling that of the number fluctuation theory, but at certain bias conditions it may reduce to a form compatible with Hooge's empirical expression. The model can unify the noise data reported in the literature, without making any ad hoc assumption on the noise generation mechanism. Specifically, the model can predict the right magnitude and bias dependence of the empirical Hooge parameter. Simulated flicker noise characteristics obtained with a circuit-simulation-oriented flicker noise model based on the new formulation were compared with experimental noise data. Excellent agreement between the calculations and measurement was observed in both the linear and saturation regions for MOS transistors fabricated by different technologies. The work shows that the flicker noise in MOS transistors can be completely explained by the trap charge fluctuation mechanism, which produces mobile carrier number fluctuation and correlated surface mobility fluctuation. >

Inhibition of the replication of hepatitis B virus in vitro by 2',3'-dideoxy-3'-thiacytidine and related analogues.

Abstract: Several 2',3'-dideoxy-3'-thiapyrimidine nucleosides were studied for their ability to inhibit hepatitis B virus (HBV) DNA replication in a HBV-transfected cell line (2.2.15). 2',3'-Dideoxy-3'-thiacytidine (SddC) and 5-fluoro-2',3'-dideoxy-3'-thiacytidine(5-FSddC) were found to be the most potent anti-HBV compounds of those examined. Both compounds resulted in nearly complete cessation of viral DNA replication at 0.5 microM, as monitored by the absence of both intracellular episomal and secreted viral DNAs. The HBV-specific RNAs were not reduced at concentrations that completely blocked HBV DNA replication, suggesting that the inhibitory target is HBV DNA synthesis. The antiviral action of SddC and 5-FSddC was reversible. The concentration of SddC and 5-FSddC required to inhibit 50% of 4-day cell growth in culture was 37 microM and more than 200 microM, respectively. Unlike 2',3'-dideoxycytidine, these two compounds do not affect mitochondrial DNA synthesis in cells at concentrations lower than that required to inhibit cell growth. In view of the potent and selective antiviral activity, both SddC and 5-FSddC should be further evaluated for the treatment of human HBV infection.

I and i

Abstract: There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality. Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

Plant Products as Antimicrobial Agents

Abstract: The use of and search for drugs and dietary supplements derived from plants have accelerated in recent years. Ethnopharmacologists, botanists, microbiologists, and natural-products chemists are combing the Earth for phytochemicals and “leads” which could be developed for treatment of infectious diseases. While 25 to 50% of current pharmaceuticals are derived from plants, none are used as antimicrobials. Traditional healers have long used plants to prevent or cure infectious conditions; Western medicine is trying to duplicate their successes. Plants are rich in a wide variety of secondary metabolites, such as tannins, terpenoids, alkaloids, and flavonoids, which have been found in vitro to have antimicrobial properties. This review attempts to summarize the current status of botanical screening efforts, as well as in vivo studies of their effectiveness and toxicity. The structure and antimicrobial properties of phytochemicals are also addressed. Since many of these compounds are currently available as unregulated botanical preparations and their use by the public is increasing rapidly, clinicians need to consider the consequences of patients self-medicating with these preparations.