Z
Zene Matsuda
Researcher at University of Tokyo
Publications - 28
Citations - 922
Zene Matsuda is an academic researcher from University of Tokyo. The author has contributed to research in topics: Lipid bilayer fusion & Gp41. The author has an hindex of 16, co-authored 28 publications receiving 725 citations. Previous affiliations of Zene Matsuda include Chinese Academy of Sciences.
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Journal ArticleDOI
The Anticoagulant Nafamostat Potently Inhibits SARS-CoV-2 S Protein-Mediated Fusion in a Cell Fusion Assay System and Viral Infection In Vitro in a Cell-Type-Dependent Manner.
Mizuki Yamamoto,Maki Kiso,Yuko Sakai-Tagawa,Kiyoko Iwatsuki-Horimoto,Masaki Imai,Makoto Takeda,Noriko Kinoshita,Norio Ohmagari,Jin Gohda,Kentaro Semba,Zene Matsuda,Yasushi Kawaguchi,Yoshihiro Kawaoka,Yoshihiro Kawaoka,Jun-ichiro Inoue +14 more
TL;DR: A quantitative fusion assay dependent on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) S protein, angiotensin I converting enzyme 2 (ACE2) and TMPRSS2 is established, and nafamostat mesylate potently inhibited the fusion while camostat Mesylate was about 10-fold less active, making it a likely candidate drug to treat COVID-19.
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Generation of a dual-functional split-reporter protein for monitoring membrane fusion using self-associating split GFP.
Hirohito Ishikawa,Hirohito Ishikawa,Fanxia Meng,Naoyuki Kondo,Naoyuki Kondo,Aikichi Iwamoto,Zene Matsuda,Zene Matsuda +7 more
TL;DR: A new methodology to facilitate generating split proteins using split GFP as a self-association module by inserting the entire GFP module at one of several candidate split points in the protein of interest, and choosing clones that retained the GFP signal and high activity relative to the original protein.
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Conformational Changes of the HIV-1 Envelope Protein during Membrane Fusion Are Inhibited by the Replacement of Its Membrane-spanning Domain
Naoyuki Kondo,Naoyuki Kondo,Kosuke Miyauchi,Fanxia Meng,Aikichi Iwamoto,Zene Matsuda,Zene Matsuda +6 more
TL;DR: In this paper, a real-time assay system employing a pair of split Renilla luciferase (spRL) fused to split green fluorescent protein (spGFP) modules was developed to provide visual reporter signals during membrane fusion.
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Role of the Specific Amino Acid Sequence of the Membrane-Spanning Domain of Human Immunodeficiency Virus Type 1 in Membrane Fusion
TL;DR: The results suggest that the MSD of gp41 has a relatively wide but not unlimited tolerance for mutations and plays a critical role in membrane fusion as well as in other steps of Env biogenesis.
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Monitoring Viral‐Mediated Membrane Fusion Using Fluorescent Reporter Methods
TL;DR: Although this unit demonstrates the application of the method for the analysis of HIV‐1 envelope protein, the reporter can be applied to analyses of various other viral envelope proteins.