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Zhihua Chen

Bio: Zhihua Chen is an academic researcher from Shanghai Jiao Tong University. The author has contributed to research in topics: Vortex & Inflammation. The author has an hindex of 37, co-authored 242 publications receiving 4623 citations. Previous affiliations of Zhihua Chen include Guangzhou Medical University & Nanjing University of Science and Technology.


Papers
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Journal ArticleDOI
TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.
Abstract: In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

1,129 citations

Journal ArticleDOI
TL;DR: The kinetic parameters of different stages were used as initial guess values for three-parallel-DAEM model calculation with good fitting quality and fast convergence rate and could be divided into three stages, which correspond to the pyrolysis of hemicellulose, cellulose and lignin, respectively.

250 citations

Journal ArticleDOI
TL;DR: In this article, pyrolysis kinetics of pine wood, rice husk and bamboo (Bambusa chungii ) were studied via thermo-gravimetric analysis technique under low heating rates.

213 citations

Journal ArticleDOI
01 Jun 2011
TL;DR: This paper proposes an effective local search algorithm based on simulated annealing and greedy search techniques to solve the traveling salesman problem and shows that the proposed algorithm provides better compromise between CPU time and accuracy among some recent algorithms for the TSP.
Abstract: The traveling salesman problem (TSP) is a classical problem in discrete or combinatorial optimization and belongs to the NP-complete classes, which means that it may be require an infeasible processing time to be solved by an exhaustive search method, and therefore less expensive heuristics in respect to the processing time are commonly used in order to obtain satisfactory solutions in short running time. This paper proposes an effective local search algorithm based on simulated annealing and greedy search techniques to solve the TSP. In order to obtain more accuracy solutions, the proposed algorithm based on the standard simulated annealing algorithm adopts the combination of three kinds of mutations with different probabilities during its search. Then greedy search technique is used to speed up the convergence rate of the proposed algorithm. Finally, parameters such as cool coefficient of the temperature, the times of greedy search, and the times of compulsive accept and the probability of accept a new solution, are adaptive according to the size of the TSP instances. As a result, experimental results show that the proposed algorithm provides better compromise between CPU time and accuracy among some recent algorithms for the TSP.

183 citations

Journal ArticleDOI
TL;DR: In this article, numerical simulations are carried out to investigate the influence of geometric parameters on the flow and heat transfer characteristics of rectangular, trapezoidal and triangular shaped microchannel heat sinks.

174 citations


Cited by
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01 Jun 2012
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
Abstract: The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.

10,124 citations

Posted Content
TL;DR: In this paper, the authors investigated conditions sufficient for identification of average treatment effects using instrumental variables and showed that the existence of valid instruments is not sufficient to identify any meaningful average treatment effect.
Abstract: We investigate conditions sufficient for identification of average treatment effects using instrumental variables. First we show that the existence of valid instruments is not sufficient to identify any meaningful average treatment effect. We then establish that the combination of an instrument and a condition on the relation between the instrument and the participation status is sufficient for identification of a local average treatment effect for those who can be induced to change their participation status by changing the value of the instrument. Finally we derive the probability limit of the standard IV estimator under these conditions. It is seen to be a weighted average of local average treatment effects.

3,154 citations

01 Mar 2017
TL;DR: Recent advances in understanding of mTOR function, regulation, and importance in mammalian physiology are reviewed and how the mTOR-signaling network contributes to human disease is highlighted.
Abstract: The mechanistic target of rapamycin (mTOR) coordinates eukaryotic cell growth and metabolism with environmental inputs, including nutrients and growth factors. Extensive research over the past two decades has established a central role for mTOR in regulating many fundamental cell processes, from protein synthesis to autophagy, and deregulated mTOR signaling is implicated in the progression of cancer and diabetes, as well as the aging process. Here, we review recent advances in our understanding of mTOR function, regulation, and importance in mammalian physiology. We also highlight how the mTOR signaling network contributes to human disease and discuss the current and future prospects for therapeutically targeting mTOR in the clinic.

2,014 citations