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Zhongqiu Liu

Bio: Zhongqiu Liu is an academic researcher from Chinese Ministry of Education. The author has contributed to research in topics: Mutant & Isothermal titration calorimetry. The author has co-authored 1 publications.

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TL;DR: In this paper, the crystal structure of the Keap1-Kelch domain with Nrf2 25-mer peptide was determined, and the molecular effects of Nrf 2Thr80 and NRF2Pro85 on the binding of Keap 1 by the method isothermal titration calorimetry (ITC), differential scanning fluorimetry, and electrophoretic mobility shift assay (EMSA).

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Xinxin Guo, Weitao Shen, Mingjun Sun, Jun Lv, Ran Liu 
TL;DR: Wang et al. as mentioned in this paper comprehensively analyzed the immune cell infiltration (ICI) and mutation genes and their combined effects for predicting prognosis in esophageal cancer (EC).
Abstract: Background Esophageal cancer (EC) had the sixth-highest mortality rate of all cancers due to its poor prognosis. Immune cells and mutation genes influenced the prognosis of EC, but their combined effect on predicting EC prognosis was unknown. In this study, we comprehensively analyzed the immune cell infiltration (ICI) and mutation genes and their combined effects for predicting prognosis in EC. Methods The CIBERSORT and ESTIMATE algorithms were used to analyse the ICI scape based on the TCGA and GEO databases. EC tissues and pathologic sections from Huai'an, China, were used to verify the key immune cells and mutation genes and their interactions. Results Stromal/immune score patterns and ICI/gene had no statistical significance in overall survival (OS) (p > 0.05). The combination of ICI and tumor mutation burden (TMB) showed that the high TMB and high ICI score group had the shortest OS (p = 0.004). We recognized that the key mutation gene NRF2 was significantly different in the high/low ICI score subgroups (p = 0.002) and positivity with mast cells (MCs) (p < 0.05). Through experimental validation, we found that the MCs and activated mast cells (AC-MCs) were more infiltration in stage II/III (p = 0.032; p = 0.013) of EC patients and that NRF2 expression was upregulated in EC (p = 0.045). AC-MCs combined with NRF2 had a poor prognosis, according to survival analysis (p = 0.056) and interactive analysis (p = 0.032). Conclusions We presume that NRF2 combined with AC-MCs could be a marker to predict prognosis and could influence immunotherapy through regulating PD-L1 in the EC.