Z
Zuzana Tothova
Researcher at Harvard University
Publications - 44
Citations - 9662
Zuzana Tothova is an academic researcher from Harvard University. The author has contributed to research in topics: Stem cell & Myeloid. The author has an hindex of 20, co-authored 40 publications receiving 8042 citations. Previous affiliations of Zuzana Tothova include Massachusetts Institute of Technology & Brigham and Women's Hospital.
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Journal ArticleDOI
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
John G. Doench,Nicolo Fusi,Meagan E Sullender,Mudra Hegde,Emma W Vaimberg,Katherine F Donovan,Ian Smith,Zuzana Tothova,Zuzana Tothova,Craig B. Wilen,Robert C. Orchard,Herbert W. Virgin,Jennifer Listgarten,David E. Root +13 more
TL;DR: Recently devised sgRNA design rules are used to create human and mouse genome-wide libraries, perform positive and negative selection screens and observe that the use of these rules produced improved results, and a metric to predict off-target sites is developed.
Journal ArticleDOI
FoxOs are critical mediators of hematopoietic stem cell resistance to physiologic oxidative stress.
Zuzana Tothova,Zuzana Tothova,Ramya Kollipara,Brian J. P. Huntly,Benjamin H. Lee,Benjamin H. Lee,Diego H. Castrillon,Dana E. Cullen,Dana E. Cullen,Elizabeth P. McDowell,Elizabeth P. McDowell,Suzan Lazo-Kallanian,Ifor R. Williams,Christopher Sears,Scott A. Armstrong,Emmanuelle Passegué,Ronald A. DePinho,D. Gary Gilliland,D. Gary Gilliland +18 more
TL;DR: FoxO proteins play essential roles in the response to physiologic oxidative stress and thereby mediate quiescence and enhanced survival in the HSC compartment, a function that is required for its long-term regenerative potential.
Journal ArticleDOI
Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
John G. Doench,Ella Hartenian,Daniel B. Graham,Zuzana Tothova,Mudra Hegde,Ian Smith,Meagan E Sullender,Benjamin L. Ebert,Ramnik J. Xavier,David E. Root +9 more
TL;DR: An online tool for the design of highly active sgRNAs for any gene of interest is provided, including a further optimization of the protospacer-adjacent motif (PAM) of Streptococcus pyogenes Cas9.
Journal ArticleDOI
FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis
Jihye Paik,Ramya Kollipara,Gerald C. Chu,Hongkai Ji,Yonghong Xiao,Zhihu Ding,Lili Miao,Zuzana Tothova,James W. Horner,Daniel R. Carrasco,Shan Jiang,D. Gary Gilliland,Lynda Chin,Wing Hung Wong,Diego H. Castrillon,Diego H. Castrillon,Ronald A. DePinho +16 more
TL;DR: The role of the mammalian FoxO transcription factor family (FoxO1, FoxO3, and FoxO4) in PI3K-AKT signaling appears to be an obligate event in the development of cancer.
Journal ArticleDOI
The retinoblastoma binding protein RBP2 is an H3K4 demethylase.
Robert J. Klose,Qin Yan,Qin Yan,Zuzana Tothova,Zuzana Tothova,Kenichi Yamane,Hediye Erdjument-Bromage,Paul Tempst,D. Gary Gilliland,D. Gary Gilliland,Yi Zhang,William G. Kaelin,William G. Kaelin +12 more
TL;DR: It is reported that RBP2 is a demethylase that specifically catalyzes demethylation on H3K4, whose methylation is normally associated with transcriptionally active genes, and the first example of a mammalian enzyme capable of erasing trimethylated H 3K4 is provided.