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Institution

Agharkar Research Institute

FacilityPune, Maharashtra, India
About: Agharkar Research Institute is a facility organization based out in Pune, Maharashtra, India. It is known for research contribution in the topics: Population & Ascorbic acid. The organization has 584 authors who have published 988 publications receiving 26768 citations.


Papers
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Journal ArticleDOI
TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.
Abstract: In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

1,129 citations

Journal ArticleDOI
TL;DR: Nanosized gold delivered DNA to plant cells while iron oxide based nanosensors detected pesticides at minute levels, assisting the development of precision farming by minimizing pollution and maximizing the value of farming practice.

754 citations

Journal ArticleDOI
TL;DR: In this paper, the size range of 2-5 nm was synthesized by a yeast strain MKY3, when challenged with 1 mM soluble silver in the log phase of growth and the nanoparticles were separated from dilute suspension by devising a new method based on differential thawing of the sample.
Abstract: Silver nanoparticles in the size range of 2-5 nm were synthesized extracellularly by a silver-tolerant yeast strain MKY3, when challenged with 1 mM soluble silver in the log phase of growth. The nanoparticles were separated from dilute suspension by devising a new method based on differential thawing of the sample. Optical absorption, transmission electron microscopy, x-ray diffraction and x-ray photoelectron spectroscopy investigations confirmed that metallic (elemental) silver nanoparticles were formed. Extracellular synthesis of nanoparticles could be highly advantageous from the point of view of synthesis in large quantities and easy downstream processing.

750 citations

Journal ArticleDOI
TL;DR: Low maternal vitamin B12 and high folate status may contribute to the epidemic of adiposity and type 2 diabetes in India.
Abstract: Aims/hypothesis Raised maternal plasma total homocysteine (tHcy) concentrations predict small size at birth, which is a risk factor for type 2 diabetes mellitus. We studied the association between maternal vitamin B12, folate and tHcy status during pregnancy, and offspring adiposity and insulin resistance at 6 years.

674 citations

Journal ArticleDOI
TL;DR: The results of this systematic study on the in vitro interactions of 7-20 nm spherical silver nanoparticles with HT-1080 and A431 cells can be used to define a safe range of SNP for the intended application as a topical antimicrobial agent after appropriate in vivo studies.

670 citations


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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20235
20226
202178
202059
201972
201848