Agilent Technologies

About: Agilent Technologies is a company organization based out in Santa Clara, California, United States. It is known for research contribution in the topics: Signal & Mass spectrometry. The organization has 7398 authors who have published 11518 publications receiving 262410 citations. The organization is also known as: Agilent Technologies, Inc..

Large dynamic range light detection

Abstract: System for large dynamic range light detection. In one aspect, the system includes a hybrid counting/integrating system for processing a signal from a photomultiplier tube. In another aspect, large dynamic range is achieved in a cascaded detector system utilizing at least one asymmetric beam splitter for delivering a larger fraction of incident light to one photomultiplier tube and for delivering a smaller fraction of the incident light to another photomultiplier tube.

Apparatus, systems and method for assaying biological materials using an annular format

Abstract: An apparatus, systems and methods use an r, θ format for the manufacture and analysis of biological materials. The apparatus is an array of discrete features of biological material in an annular region on a substrate. The apparatus is formed with a system for synthesizing arrays that includes a spinner assembly for rotating the substrate during the synthesis of the biological material thereon. The spinner assembly provides efficient means for printing an annular array pattern, spreading and removing ancillary fluids used in the synthesis process. The apparatus is hybridized with complementary biological material using a system for hybridizing that includes a spinner assembly to spin the apparatus after the complementary biological material is added. The spinning motion spreads the complementary biological material efficiently over the annular array such that a much smaller amount of complementary biological material is needed for the assay. The spinning motion effectively removes unhybridized material and ancillary wash fluids and moves any bubbles that form out of the array region on the substrate. The hybridized apparatus is optically interrogated with a system for interrogation that includes a light source, optics, and a scanning assembly that holds and rotates the apparatus so that the light source can remain stationary. The hybridized apparatus is interrogated in an r, θ format using the rotating scanning assembly. The interrogation system further comprises a linear stage to move the apparatus or the optics radially to efficiently expose all features in the annular region of the apparatus to the light source. Rotation of the substrate in the synthesis, hybridization and optical interrogation of the array provides many advantages to the process of assaying biological materials that are not found in conventional systems using an x, y format. The method of assaying biological materials uses the systems for synthesizing, hybridizing and optically interrogating the apparatus in accordance with the invention.

Combined pointing device and bar code scanner

Abstract: A computer mouse uses an optical sensor to detect movement and thereby generate signals to control movement of an on-screen icon. The optical mouse also includes circuitry or a processor to detect bar codes and demodulate data encoded therein.

Development of Comprehensive Online Two-Dimensional Liquid Chromatography/Mass Spectrometry Using Hydrophilic Interaction and Reversed-Phase Separations for Rapid and Deep Profiling of Therapeutic Antibodies.

Abstract: Monoclonal antibodies (mAb) and related molecules are being developed at a remarkable pace as new therapeutics for the treatment of diseases ranging from cancer to inflammatory disorders. However, characterization of these molecules at all stages of development and manufacturing presents tremendous challenges to existing analytical technologies because of their large size (ca. 150 kDa) and inherent heterogeneity resulting from complex glycosylation patterns and other post-translational modifications. Multidimensional liquid chromatography is emerging as a powerful platform technology that can be used to both improve analysis speed for these molecules by combining existing one-dimensional separations into a single method (e.g., Protein A affinity separation and size-exclusion chromatography) and increasing the resolving power of separations by moving from one dimension of separation to two. In the current study, we have demonstrated the ability to combine hydrophilic interaction (HILIC) and RP separations in an online comprehensive 2D separation coupled with high resolution MS detection (HILIC × RP-HRMS). We find that active solvent modulation (ASM) is critical for coupling these two separation modes, because it mitigates the otherwise serious negative impact of the acetonitrile-rich HILIC mobile phase on the second dimension RP separation. The chromatograms obtained from these HILIC × RP-HRMS separations of mAbs at the subunit level reveal the extent of glycosylation on the Fc/2 and Fd subunits in analysis times on the order of 2 h. In comparison to previous CEX × RP separations of the same molecules, we find that chromatograms from the HILIC × RP separations are richer and reveal separation of some glycoforms that coelute in the CEX × RP separations.