Agilent Technologies

About: Agilent Technologies is a company organization based out in Santa Clara, California, United States. It is known for research contribution in the topics: Signal & Mass spectrometry. The organization has 7398 authors who have published 11518 publications receiving 262410 citations. The organization is also known as: Agilent Technologies, Inc..

Bulk acoustic wave resonator with improved lateral mode suppression

Abstract: A bulk acoustic wave device that provides a high spectral purity, high Q, resonator in the radio frequency and microwave frequency ranges. Such resonators may be coupled together to form filters or other frequency selective devices. The bulk acoustical wave filter is constructed from a piezoelectric (PZ) material having a first surface and a second surface and first and second electrodes. The first electrode includes an electrically conducting layer on the first surface, and the second electrode includes an electrically conducting layer on the second surface. The first electrode overlies at least a portion of the second electrode, the portion of the first electrode that overlies the second electrode has a periphery which is a non-rectangular, irregular polygon. In the preferred embodiment of the present invention, the periphery is a three-sided, four-sided, or n-sided irregular polygon in which no two sides are parallel to one another.

Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis.

Abstract: Generation of complex libraries of defined nucleic acid sequences can greatly aid the functional analysis of protein and gene function. Previously, such studies relied either on individually synthesized oligonucleotides or on cellular nucleic acids as the starting material. As each method has disadvantages, we have developed a rapid and cost-effective alternative for construction of small-fragment DNA libraries of defined sequences. This approach uses in situ microarray DNA synthesis for generation of complex oligonucleotide populations. These populations can be recovered and either used directly or immortalized by cloning. From a single microarray, a library containing thousands of unique sequences can be generated. As an example of the potential applications of this technology, we have tested the approach for the production of plasmids encoding short hairpin RNAs (shRNAs) targeting numerous human and mouse genes. We achieved high-fidelity clone retrieval with a uniform representation of intended library sequences.

Noninvasive blood chemistry measurement method and system

Abstract: A noninvasive blood chemistry measurement method and system isolate measurement contributions due to a patient's blood to accurately measure blood chemistry. In accordance with a first preferred embodiment of the present invention a noninvasive blood chemistry measurement method decreases the blood volume within a patient's body part relative to the normal blood volume in the body part and performs a baseline measurement. Blood volume is then increased and a second measurement is performed. Comparison of the second measurement to the baseline measurement isolates the measurement attributes of the patient's blood. In accordance with a second preferred embodiment of the present invention a noninvasive blood chemistry measurement system decreases blood volume by applying mechanical pressure to a body part. In accordance with a third preferred embodiment of the present invention, blood volume in the body part is decreased using a pressure cuff. In a fourth embodiment, a noninvasive probe accurately measures blood chemistry and uses a suction cup to increase blood volume at the blood chemistry measurement site.

Physiological and molecular adaptations to drought in Andean potato genotypes

Abstract: The drought stress tolerance of two Solanum tuberosum subsp. andigena landraces, one hybrid (adgxtbr) and Atlantic (S. tuberosum subsp. tuberosum) has been evaluated. Photosynthesis in the Andigena landraces during prolonged drought was maintained significantly longer than in the Tuberosum (Atlantic) line. Among the Andigena landraces, 'Sullu' (SUL) was more drought resistant than 'Negra Ojosa' (NOJ). Microarray analysis and metabolite data from leaf samples taken at the point of maximum stress suggested higher mitochondrial metabolic activity in SUL than in NOJ. A greater induction of chloroplast-localized antioxidant and chaperone genes in SUL compared with NOJ was evident. ABA-responsive TFs were more induced in NOJ compared with SUL, including WRKY1, mediating a response in SA signalling that may give rise to increased ROS. NOJ may be experiencing higher ROS levels than SUL. Metabolite profiles of NOJ were characterized by compounds indicative of stress, for example, proline, trehalose, and GABA, which accumulated to a higher degree than in SUL. The differences between the Andigena lines were not explained by protective roles of compatible solutes; hexoses and complex sugars were similar in both landraces. Instead, lower levels of ROS accumulation, greater mitochondrial activity and active chloroplast defences contributed to a lower stress load in SUL than in NOJ during drought.

Chemical array fabrication with identifier

Abstract: A method of generating an addressable array of biopolymers, such as DNA probes, on a substrate. The method includes receiving from a remote station, information on a layout of the array and an associated first identifier. A local identifier is generated corresponding to the first identifier and associated array, the local identifier being shorter in length than the corresponding first identifier. The addressable array is fabricated on the substrate in accordance with the received layout information. A first copy of the local identifier is applied to the substrate or a housing carrying the substrate. The fabricated array and the first copy of the local identifier are shipped to a remote user station. A method at the corresponding user station, and apparatus and computer program products useful at either station, are further provided.