Agriculture and Agri-Food Canada

About: Agriculture and Agri-Food Canada is a facility organization based out in Ottawa, Ontario, Canada. It is known for research contribution in the topics: Population & Soil water. The organization has 10921 authors who have published 21332 publications receiving 748193 citations. The organization is also known as: Department of Agriculture and Agri-Food.

Nitrogen fertilization management for no-till cereal production in the Canadian Great Plains: a review

Abstract: Nitrogen (N) is the nutrient most limiting crop production in all areas of the world and is generally applied to soil in the largest quantity. A review of the research on N fertilization management for no-till cereal production in the Canadian Great Plains, on mainly Chernozem and Gray soils, was done to illustrate the management practices which can be used to optimize the N use efficiency so as to minimize the N loss from root zone and environmental damage. Applied N is subject to loss by volatilization, immobilization, denitrification and leaching in soil and its efficiency of use by plants is governed by soil and climatic factors, fertilizer material, and soil, crop and fertilizer management practices. Overall efficiency of applied N has been <70%. Reducing tillage intensity modifies both the demand of crops for N due to changes in yield potential, and the supply of N due to changes in N cycling and losses. Consequently, it may be necessary to compensate for this by adjusting the fertilizer rate. Fertilizer use efficiency may also change with changes in tillage management, microclimate, microbial activity and distribution of fertilizer relative to crop residue. Placing the fertilizer in a band reduces contact with soil microorganisms, reducing immobilization of both ammonium (NH4) and nitrate (NO3). Banding also slows the conversion of urea to NH3 and NH4 to NO3, which can reduce losses by denitrification and leaching. The use of the urease inhibitor n-(n-butyl) thiophosphoric triamide (NBPT) shows promise in improving the efficiency of surface-applied urea-containing fertilizers in no-till systems and reducing seedling damage from seed-placed fertilizers. Ultimately, any N fertilization package has advantages and disadvantages. In selecting the optimum fertilizer management system for a farming operation, the balance between rate of application, cost and availability of equipment, soil disturbance, seedbed quality, moisture conservation, time and labor constraints and fertilizer use efficiency must be considered. The “best” management system is not fixed, but depends on the major limiting factors on each individual farm.

Expression of a Phytophthora sojae necrosis-inducing protein occurs during transition from biotrophy to necrotrophy

Abstract: Phytophthora sojae is an oomycete that causes stem and root rot on soybean plants. To discover pathogen factors that produce disease symptoms or activate plant defense responses, we identified putative secretory proteins from expressed sequence tags (ESTs) and tested selected candidates using a heterologous expression assay. From an analysis of 3035 ESTs originating from mycelium, zoospore, and infected soybean tissues, we identified 176 putative secreted proteins. A total of 16 different cDNAs predicted to encode secreted proteins ranging in size from 6 to 26 kDa were selected for expression analysis in Nicotiana benthamiana using an Agrobacterium tumefaciens binary potato virus X (PVX) vector. This resulted in the identification of a 25.6-kDa necrosis-inducing protein that is similar in sequence to other proteins from eukaryotic and prokaryotic species. The genomic region encoding the P. sojae necrosis-inducing protein was isolated and the expression pattern of the corresponding gene determined by RNA blot hybridization and by RT-PCR. The activity of this P. sojae protein was compared to proteins of similar sequence from Fusarium oxysporum, Bacillus halodurans, and Streptomyces coelicolor by PVX-based expression in N. benthamiana and by transient expression via particle bombardment in soybean tissues. The P. sojae protein was a powerful inducer of necrosis and cell death in both assays, whereas related proteins from other species varied in their activity. This study suggests that the P. sojae necrosis-inducing protein facilitates the colonization of host tissues during the necrotrophic phase of growth.