Showing papers by "Albion College published in 2015"

The Impact of Host Diet on Wolbachia Titer in Drosophila

Abstract: While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

Drosophila Muller F Elements Maintain a Distinct Set of Genomic Properties Over 40 Million Years of Evolution

Abstract: The Muller F element (4.2 Mb, ~80 protein-coding genes) is an unusual autosome of Drosophila melanogaster; it is mostly heterochromatic with a low recombination rate. To investigate how these properties impact the evolution of repeats and genes, we manually improved the sequence and annotated the genes on the D. erecta, D. mojavensis, and D. grimshawi F elements and euchromatic domains from the Muller D element. We find that F elements have greater transposon density (25–50%) than euchromatic reference regions (3–11%). Among the F elements, D. grimshawi has the lowest transposon density (particularly DINE-1: 2% vs. 11–27%). F element genes have larger coding spans, more coding exons, larger introns, and lower codon bias. Comparison of the Effective Number of Codons with the Codon Adaptation Index shows that, in contrast to the other species, codon bias in D. grimshawi F element genes can be attributed primarily to selection instead of mutational biases, suggesting that density and types of transposons affect the degree of local heterochromatin formation. F element genes have lower estimated DNA melting temperatures than D element genes, potentially facilitating transcription through heterochromatin. Most F element genes (~90%) have remained on that element, but the F element has smaller syntenic blocks than genome averages (3.4–3.6 vs. 8.4–8.8 genes per block), indicating greater rates of inversion despite lower rates of recombination. Overall, the F element has maintained characteristics that are distinct from other autosomes in the Drosophila lineage, illuminating the constraints imposed by a heterochromatic milieu.

Green Synthesis of Metal Nanoparticles via Natural Extracts: The Biogenic Nanoparticle Corona and Its Effects on Reactivity

Abstract: The optical and catalytic properties of metal nanoparticles have attracted significant attention for applications in a wide variety of fields, thus prompting interest in developing sustainable synthetic strategies that leverage the redox properties of natural compounds or extracts. Here, we investigate the surface chemistry of nanoparticles synthesized using coffee as a biogenic reductant. Building on our previously developed synthetic protocols for the preparation of silver and palladium nanoparticle/carbon composite microspheres, a combination of thermogravimetric and spectroscopic methods was used to characterize the carbon microsphere and nanoparticle surfaces. Infrared reflectance spectroscopy and single particle surface enhanced Raman spectroscopy were used to characterize Pd and Ag metal surfaces, respectively, following synthesis. Strongly adsorbed organic layers were found to be present at metal nanoparticle surfaces after synthesis. The catalytic activity of Pd nanoparticles in hydrogenation rea...

Observations of Binary Stars with the Differential Speckle Survey Instrument. VI. Measures during 2014 at the Discovery Channel Telescope

Abstract: We present the results of 938 speckle measures of double stars and suspected double stars drawn mainly from the Hipparcos Catalog, as well as 208 observations where no companion was noted. One hundred fourteen pairs have been resolved for the first time. The data were obtained during four observing runs in 2014 using the Differential Speckle Survey Instrument at Lowell Observatory's Discovery Channel Telescope. The measurement precision obtained when comparing to ephemeris positions of binaries with very well-known orbits is generally less than 2 mas in separation and 05 in position angle. Differential photometry is found to have internal precision of approximately 0.1 mag and to be in very good agreement with Hipparcos measures in cases where the comparison is most relevant. We also estimate the detection limit in the cases where no companion was found. Visual orbital elements are derived for six systems.

Nascent Transcription Affected by RNA Polymerase IV in Zea mays

Abstract: All eukaryotes use three DNA-dependent RNA polymerases (RNAPs) to create cellular RNAs from DNA templates. Plants have additional RNAPs related to Pol II, but their evolutionary role(s) remain largely unknown. Zea mays (maize) RNA polymerase D1 (RPD1), the largest subunit of RNA polymerase IV (Pol IV), is required for normal plant development, paramutation, transcriptional repression of certain transposable elements (TEs), and transcriptional regulation of specific alleles. Here, we define the nascent transcriptomes of rpd1 mutant and wild-type (WT) seedlings using global run-on sequencing (GRO-seq) to identify the broader targets of RPD1-based regulation. Comparisons of WT and rpd1 mutant GRO-seq profiles indicate that Pol IV globally affects transcription at both transcriptional start sites and immediately downstream of polyadenylation addition sites. We found no evidence of divergent transcription from gene promoters as seen in mammalian GRO-seq profiles. Statistical comparisons identify genes and TEs whose transcription is affected by RPD1. Most examples of significant increases in genic antisense transcription appear to be initiated by 3ʹ-proximal long terminal repeat retrotransposons. These results indicate that maize Pol IV specifies Pol II-based transcriptional regulation for specific regions of the maize genome including genes having developmental significance.

A Case Study of Liberal Arts Colleges in the 21st Century: Understanding Organizational Change and Evolution in Higher Education

Abstract: We draw upon the evolutionary model of change in order to examine the organizational transformation of three liberal arts colleges (Albion College, Allegheny College, Kenyon College). Relying on our prior research (Baker, Baldwin, & Makker, 2012), we seek to continue our exploration and understanding of the evolution occurring in the important liberal arts college sector of higher education. We seek to understand why and how these colleges change, what changes occur, and, especially, what makes liberal arts colleges susceptible to change. The findings of this study have the potential to illuminate change in other types of higher education institutions.

Project FIT: A School, Community and Social Marketing Intervention Improves Healthy Eating Among Low-Income Elementary School Children

Abstract: Project FIT was a two-year multi-component nutrition and physical activity intervention delivered in ethnically-diverse low-income elementary schools in Grand Rapids, MI. This paper reports effects on children's nutrition outcomes and process evaluation of the school component. A quasi-experimental design was utilized. 3rd, 4th and 5th-grade students (Yr 1 baseline: N = 410; Yr 2 baseline: N = 405; age range: 7.5-12.6 years) were measured in the fall and spring over the two-year intervention. Ordinal logistic, mixed effect models and generalized estimating equations were fitted, and the robust standard errors were utilized. Primary outcomes favoring the intervention students were found regarding consumption of fruits, vegetables and whole grain bread during year 2. Process evaluation revealed that implementation of most intervention components increased during year 2. Project FIT resulted in small but beneficial effects on consumption of fruits, vegetables, and whole grain bread in ethnically diverse low-income elementary school children.

Antecedents and outcomes: theories of fit and the study of doctoral education

Abstract: This paper explores fit as an important theoretical construct in the study of doctoral education and doctoral student development. We discuss how research based on three types of fit (person–environment fit, person–culture fit, person–vocation fit) may provide critical insights into the doctoral student experience, and offer a framework based on antecedents and outcomes to support future research. We conclude with an application of this framework to two understudied populations of doctoral students and future research directions.

Faculty as Mentors in Undergraduate Research, Scholarship, and Creative Work: Motivating and Inhibiting Factors

Abstract: In this study, we sought to contribute to research about the high-impact practice of undergraduate research from the understudied faculty perspective. We relied on focus group data from faculty members (N = 41) across five institutions to better understand the supporting and inhibiting factors that contribute to faculty members’ engagement in mentoring undergraduate research. We offer implications for research and practice.

Facebook Use and Acculturation: The Case of Overseas Chinese Professionals in Western Countries

Abstract: The emergence of social network sites has provided new opportunities for intercultural communication. This study is one of the first to explore the role of Facebook on the acculturation of Chinese professionals overseas. Through qualitative interviews, we explored how overseas Chinese professionals use Facebook to maintain their social networks, manage their multicultural identities, and adapt to Western culture in their host countries. Our research reveals that overseas Chinese professionals tended to manage their identity with little self-awareness, and their Facebook communication reflected Chinese traditional culture in many ways. Facebook was regarded as a useful acculturation tool for them to learn about popular social topics in the host countries.

Borings in early Eocene turtle shell from the Wasatch Formation, South Pass, Wyoming

Abstract: Borings in fossil turtle shells collected from the lowermost beds of the early Eocene Cathedral Bluffs Tongue of the Wasatch Formation in the northwestern part of the Green River Basin near South Pass, Wyoming, are herein described. Individual turtle shells in the study area are characterized by as few as one or two and as many as >100 borings. The borings include both non-penetrative forms (those which do not pass fully though the shell) as well as penetrative forms (those which pass fully from the exterior to the interior surface of the shell). All non-penetrative forms occur on external surfaces of the carapace and plastron (i.e. those that would have been accessible while the host taxon was alive). Two new ichnogenera and four new ichnospecies are established to describe these borings. Karethraichnus (new ichnogenus) includes three ichnospecies: K. lakkos (new ichnospecies), K. kulindros (new ichnospecies), and K. fiale (new ichnospecies). Karethraichnus lakkos are shallow (non-penetrating), hemispherical pits with rounded, to flattened bases. Karethraichnus kulindros are deep, non-penetrative traces with a cylindrical profile, an axis approximately perpendicular to the substrate surface and with rounded to flattened, hemispherical termini. Karethraichnus fiale are penetrative traces with a cylindrical to bi-convex or flask-shaped profile, and an axis approximately perpendicular to the substrate surface. Thatchtelithichnus (new ichnogenus) Thatchtelithichnus holmani (new ichnospecies) consist of non-penetrative borings into a bone substrate. They consist of a ring-shaped trace, with a central pedestal or platform. The position of the borings on the shells, and evidence of syn-emplacement healing of the borings in several of the turtles, indicates that these borings were emplacement by ectoparasites/mesoparasites while the animals were living. Similar traces in modern emydid turtles are attributed to ticks, leeches, or spirorchid liver flukes.

Academic Departments as Networks of Informal Learning: Faculty Development at Liberal Arts Colleges.

Abstract: In this article, we consider the role of departmental contexts and relationships in faculty work within liberal arts colleges. Knowledge about how departmental networks relate to success and satisfaction may inform the work of those who support faculty work in liberal arts colleges, as well as other institution types. Analysis of quantitative and qualitative survey data from faculty members at 13 liberal arts colleges in the US suggests that informal departmental relationships influence faculty experiences. Findings suggest that departmental leaders and colleagues are sources of professional development and support, but that counter-productive behaviours interfere with such support and foster negative workplace cultures.

People Strategy in Human Resources: Lessons for Mentoring in Higher Education

Abstract: In this article, I offer the notion of alignment, a human resources framework, as a conceptual tool for better informing the development of mentoring programming and policy in higher education. Alignment accounts for both individual and organizational factors as means for providing the necessary connections among human resources and organizational policies and outcomes. The three types of alignment: Vertical (connection between people strategy and business goals), horizontal (connection among individual HR policy areas), and implementation (the degree to which action is taken to put the people strategy into effect) have the potential to improve the efficacy of mentorships, the contextual factors that influences them, and the intended outcomes of these relationships.

Isolation, characterization and community diversity of indigenous putative toluene-degrading bacterial populations with catechol-2,3-dioxygenase genes in contaminated soils.

Abstract: Indigenous bacterial assemblages with putative hydrocarbon-degrading capabilities were isolated, characterized and screened for the presence of the catechol-2,3-dioxygenase (C23O) gene after exposure to toluene in two different (i.e., pristine and conditioned) soil communities. The indigenous bacterial populations were exposed to the hydrocarbon substrate by the addition of toluene concentrations, ranging from 0.5 % to 10 % V/W in 10 g of each soil and incubated at 30 °C for upwards of 12 days. In total, 25 isolates (11 in pristine soil and 14 in conditioned soil) were phenotypically characterized according to standard microbiological methods and also screened for the 238-bp C23O gene fragment. Additionally, 16S rRNA analysis of the isolates identified some of them as belonging to the genera Bacillus, Exiguobacterium, Enterobacter, Pseudomonas and Stenotrophomonas. Furthermore, the two clone libraries that were constructed from these toluene-contaminated soils also revealed somewhat disparate phylotypes (i.e., 70 % Actinobacteria and Firmicutes to 30 % Proteobacteria in conditioned soil, whereas in pristine soil: 66 % Actinobacteria and Firmicutes; 21 % Proteobacteria and 13 % Bacteroidetes). The differences observed in bacterial phylotypes between these two soil communities may probably be associated with previous exposure to hydrocarbon sources by indigenous populations in the conditioned soil as compared to the pristine soil.

Miconia abscondita (Melastomataceae: Miconieae), A New Species from the Massif De La Hotte, Haiti: Rediscovered in Herbaria After Being Hidden for Nearly Nine Decades

Abstract: In the course of a taxonomic revision of several clades of Greater Antillean Melastomataceae, we discovered a previously undescribed species, Miconia abscondita, from the Massif de la Hotte, Haiti. Miconia abscondita is based on a vegetative collection by Swedish botanist, E. L. Ekman, which was made in 1926. This new species is a member of the Mecranium clade, a group well supported on the basis of both morphological and molecular data, but which is highly embedded within the phylogenetic structure of the genus Miconia. Miconia abscondita is clearly placed within the Mecranium clade on the basis of DNA sequence data, and this placement also is supported by the form of its hairs and, especially, the pattern of stem indumentum; however, it matches no currently described species. Miconia abscondita is thus described, illustrated, and compared with putatively related and/or phenetically similar species. The discovery of M. abscondita brings to 25 the number of recognized species within the Mecranium...

Observations of Binary Stars with the Differential Speckle Survey Instrument. VI. Measures During 2014 at the Discovery Channel Telescope

Abstract: We present the results of 938 speckle measures of double stars and suspected double stars drawn mainly from the Hipparcos Catalogue, as well as 208 observations where no companion was noted. One hundred fourteen pairs have been resolved for the first time. The data were obtained during four observing runs in 2014 using the Differential Speckle Survey Instrument (DSSI) at Lowell Observatory's Discovery Channel Telescope. The measurement precision obtained when comparing to ephemeris positions of binaries with very well-known orbits is generally less than 2 mas in separation and 0.5 degrees in position angle. Differential photometry is found to have internal precision of approximately 0.1 magnitudes and to be in very good agreement with Hipparcos measures in cases where the comparison is most relevant. We also estimate the detection limit in the cases where no companion was found. Visual orbital elements are derived for 6 systems.

Systems Alignment for Comprehensive Faculty Development in Liberal Arts Colleges.

Abstract: Using an alignment framework, the authors explore faculty development initiatives in liberal arts colleges in order to understand the connection between organizational priorities and processes as connected to faculty members’ stated needs. The study draws on mixed-methods data from The Initiative for Faculty Development in Liberal Arts Colleges (IFDLAC), including survey and interview data from the 13 member institutions of the Great Lakes Colleges Association (GLCA).The authors offer future implications for faculty development practice.

A social marketing approach to promoting healthful eating and physical activity in low-income and ethnically diverse schools

Abstract: Objective:To evaluate the short-term outcome of the social marketing approach used in Project FIT, we developed a school- and community-based programme for promoting healthful eating and physical a...

A Good Story: Using Future Life Narratives to Predict Present Well-Being

Abstract: The present research examined the predictive and nomological validity of a narrative method for assessing goals. College students (N = 337, 158 women, M age = 19.08) from a large, public university wrote short narratives about their best possible selves in the future, imagining that they had realized all of their life dreams. Narratives were coded in terms of the number of statements reflecting each of fourteen types of goals. Intercoder reliability was strong. With regard to predictive validity, intrinsic goals, particularly spiritual and intimacy goals were positively related to well-being. Extrinsic goals, power goals in particular, tended to be negatively related to well-being. With regard to nomological validity, the spiritual goals-well-being relationship was mediated by frequency of religious service attendance and self-report measures of religiosity. Interestingly, intrinsic goals were negatively related to life satisfaction. Results are discussed in the context of self-determination theory and the internalization of extrinsic motivations.

Reactivity and Survivability of Glycolaldehyde in Simulated Meteorite Impact Experiments

Abstract: Sugars of extraterrestrial origin have been observed in the interstellar medium (ISM), in at least one comet spectrum, and in several carbonaceous chondritic meteorites that have been recovered from the surface of the Earth. The origins of these sugars within the meteorites have been debated. To explore the possibility that sugars could be generated during shock events, this paper reports on the results of the first laboratory impact experiments wherein glycolaldehyde, found in the ISM, as well as glycolaldehyde mixed with montmorillonite clay, have been subjected to reverberated shocks from ~5 to >25 GPa. New biologically relevant molecules, including threose, erythrose and ethylene glycol, were identified in the resulting samples. These results show that sugar molecules can not only survive but also become more complex during impact delivery to planetary bodies.

Haiti and the Dominican Republic

Abstract: Walter S. Judd, Teodoro Clase, J. Dan Skean, Jr., and Lucas C. Majure University of Florida, Department of Biology, 220 Bartram Hall, P.O. Box 118525, Gainesville, Florida 32611 Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 Jardin Botanico Nacional Dr. Rafael M. Moscoso, Apartado 21-9, Santo Domingo, Republica Dominicana Albion College, Department of Biology, Albion, Michigan 49224 Desert Botanical Garden, Department of Research, Conservation, and Collections, 1201 North Galvin Parkway, Phoenix, Arizona 85008

Investigating the Potential of Community College Developmental Summer Bridge Programs in Facilitating Student Adjustment to Four-Year Institutions

Abstract: This study examines whether community college developmental summer bridge programs (DSBPs) can help students acquire the academic and college knowledge needed to attend and succeed at a four-year higher education institution. In-depth interviews with 14 participants at nine four-year institutions in Texas were conducted two years after their random assignment into one of four four-week long community college DSBPs. The interviews reinforced the value of such programs in affording key validating and socializing experiences as well as illuminate program limitations in managing student expectations and providing institution-specific college knowledge for students who will matriculate at a four-year institution subsequent to program participation.

Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesis

Abstract: Physically separating daughter cells during cytokinesis requires contraction of an actin-myosin ring and vesicle-mediated membrane addition at the cleavage furrow. To identify vesicle trafficking proteins that function in cytokinesis, we screened deficiencies and mutations of candidate genes by live imaging the mitotic domains of the Drosophila embryo. In embryos homozygous for some of these deficiencies, we observed several cytokinesis phenotypes, including slow furrow ingression and increased membrane blebbing. We also found that cytokinesis required the Sec1/Munc18 homolog Rop, which interacts with syntaxin and mediates exocytosis at the plasma membrane. In a temperature-sensitive Rop mutant (Rop(TS)), the contractile ring disassembled during furrow ingression, indicating that maintenance of the ring required vesicle addition. Furthermore, in some dividing Rop(TS) cells, the shape of the daughter cells became unstable, causing cytokinesis failure. These results further highlight the importance of vesicle trafficking in animal cytokinesis and show that vesicle fusion influences cell shape during cytokinesis.

What About the Rest of Us? The Importance of Organizational Culture in Nepotistic Environments

Abstract: To date, empirical investigations into whether nepotism, specifically, or social connection preference (SCP), generally, is positive or negative within the realm of organizational scholarship has been limited. Indeed, most of the early discussions in this field on the subject have focused on previous work done outside the organizational area (i.e., Bellow, 2003). A recent Society for Industrial and Organizational Psychology Organizational Frontier Series book on the subject has brought some initial attention to the subject by approaching it from multiple domains within the organizational literature (Jones, 2012). Jones and Stout (2015) have highlighted the possible advantages and disadvantages of SCPs and have taken the stand that SCPs might in fact provide more benefits than drawbacks, particularly given that formalized policies may lead to unfair discrimination.

Radiance line ratios Ly-β/Ly-α, Ly-γ/Ly-α, Ly-δ/Ly-α, and Ly-ε/Ly-α for soft X-ray emissions following charge exchange between C6+ and Kr

Abstract: The radiance line ratios Ly-β/Ly-α, Ly-γ/Ly-α, Ly-δ/Ly-α, and Ly-e/Ly-α for soft X-ray emission following charge exchange (CX) between C6+ and Kr are reported for collision energies between approximately 320 and 46,000 eV/u. The corresponding collision velocities (250–3000 km/s) are characteristic of the solar wind. X-ray spectra were obtained at the Oak Ridge National Laboratory Multicharged Ion Research Facility using a microcalorimeter X-ray detector with a resolution on the order of 10 eV FWHM. The measured Ly-e/Ly-α is zero for all considered energies and suggests that very little, if any, capture to 6p occurs. The measured Ly-β/Ly-α and Ly-γ/Ly-α ratios intersect and form a well resolved node around (950 ± 50) km/s, which could be used as an astrophysical velocity indicative tool. The results reported here are compared to calculations for C6+ + H since no published theory for C6+ + Kr is known to exist. Double-electron-capture (DEC) and other multi-electron processes are possible. True double capture is estimated to be only 10% of the single-electron-capture (SEC).

Reproducibility of the Veterans Physical Activity Questionnaire in an elderly population.

Abstract: Background: Quantifying lifetime physical activity using self-reported measures is challenging due to reliance on recall, especially in older populations. The purpose of this study was to determine the 1-year reproducibility of the Veterans Physical Activity Questionnaire (VAPAQ) in a cohort of patients with documented abdominal aortic aneurysm disease (AAA). Methods: Subjects included men (n = 52) and women (n = 3) enrolled in AAA STOP, a randomized trial designed to test the ability of supervised exercise training to modify AAA biology and early disease progression. Results: The overall correlation coefficient for lifetime recreational energy expenditure between the 2 examinations was 0.93 (P < .001), with an overall difference of 26 kcal/week, a typical error (standard deviation of the differences) of 171 kcals/week, and a coefficient of variation (CV) of 15.5%. Conclusions: The VAPAQ is a reproducible tool to quantify lifetime energy expenditure in older adults with documented vascular disease.