Aligarh Muslim University

About: Aligarh Muslim University is a education organization based out in Aligarh, Uttar Pradesh, India. It is known for research contribution in the topics: Population & Adsorption. The organization has 8218 authors who have published 16416 publications receiving 289068 citations. The organization is also known as: AMU.

Infected foot ulcers in male and female diabetic patients: a clinico-bioinformative study

Abstract: The study aimed at (i) characterizing the mode of transmission of blaCTX-M and blaTEM-1 among extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains isolated from infected diabetic foot ulcers, and (ii) identifying the risk factors for "sex-associated multidrug resistant Gram-negative bacterial (MDRGNB)-infection status" of the ulcers. Seventy-seven diabetic patients having clinically infected foot ulcers were studied in a consecutive series. The E. coli strains isolated from the ulcers were screened for blaCTX-M, blaTEM-1, armA, rmtA and rmtB during the 2-year study-period. PCR amplified blaCTX-M genes were cloned and sequenced. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for the analysis of genetic relatedness of the ESBL-producers. Risk factors for "sex-associated MDRGNB-infection status" of ulcers were assessed. Modeling was performed using Swiss-Model-Server and verified by Procheck and verify3D programmes. Discovery Studio2.0 (Accelrys) was used to prepare Ramachandran plots. Z-scores were calculated using 'WHAT IF'-package. Docking of cefotaxime with modeled CTX-M-15 enzyme was performed using Hex5.1. Among 51 E. coli isolates, 14 (27.5%) ESBL-producers were identified. Only 7 Class1 integrons, 2 blaCTX-M-15, and 1 blaTEM-1 were detected. Ceftazidime and cefotaxime resistance markers were present on the plasmidic DNA of both the blaCTX-M-15 positive strains and were transmissible through conjugation. The residues Asn132, Glu166, Pro167, Val172, Lys234 and Thr235 of CTX-M-15 were found to make important contacts with cefotaxime in the docked-complex. Multivariate analysis proved 'Glycemic control at discharge' as the single independent risk factor. Male diabetic patients with MDRGNB-infected foot ulcers have poor glycemic control and hence they might have higher mortality rates compared to their female counterparts. Plasmid-mediated conjugal transfer, albeit at a low frequency might be the possible mechanism of transfer of blaCTX-M-15 resistance marker in the present setting. Since the docking results proved that the amino acid residues Asn132, Glu166, Pro167, Val172, Lys234 and Thr235 of CTX-M-15 (enzyme) make important contacts with cefotaxime (drug) in the 'enzyme-drug complex', researchers are expected to duly utilize this information for designing more potent and versatile CTX-M-inhibitors.

Applications of Hg(II) sensitive polyaniline Sn(IV) phosphate composite cation-exchange material in determination of Hg2+ from aqueous solutions and in making ion-selective membrane electrode

Abstract: Polyaniline Sn(IV) phosphate an ‘organic–inorganic’ composite material, was prepared via sol–gel mixing of organic polymer polyaniline into the matrices of the inorganic precipitate of Sn(IV) phosphate The ion-exchange capacity and distribution coefficients with respect to various metal ions were determined on the column of polyaniline Sn(IV) phosphate The distribution studies showed the selectivity of Hg(II) ions by this material However, the sensitivity level of Hg(II) ions on the composite material was determined quantitatively Hg(II) ions were separated and determined from the binary aqueous mixtures of Hg(II)–Cu(II), Hg(II)–Ni(II), Hg(II)–Cd(II), Hg(II)–Co(II), Hg(II)–Al(III), etc For the determination of Hg(II) ions in aqueous solutions, a Hg(II) ion sensitive membrane electrode was prepared by means of this composite cation-exchanger as the electroactive material The membrane electrode was mechanically stable with quick response time and could be operated over a wide pH range with a slope of 30 mV per decade change in a linear concentration range of 1 × 10−1 to 1 × 10−6 M The selectivity coefficients were determined by the mixed solution method and revealed that the electrode was selective for Hg(II) in the presence of interfering cations The practical utility of this electrode was established by employing it as an indicator electrode in the potentiometric titration of Hg(II)

PECTIN BASED CERIUM (IV) AND THORIUM (IV) PHOSPHATES AS NOVEL HYBRID FIBROUS ION EXCHANGERS Synthesis, characterization and thermal behaviour

Abstract: Pectin based cerium (IV) and thorium (IV) phosphates have been synthesized as new phases of hybrid fibrous ion exchangers. Both materials were characterized using X-ray diffraction, infrared (IR) spectra, thermogravimetric analysis (TG), differntial thermogravimetry (DTG), differntial thermal analysis (DTA) and scanning electron microscopy (SEM), as well as the determination of their ion exchange capacity, elution and pH titration. The X-ray study reveals the amorphous nature of the materials, while SEM studies confirm the fibrous nature of the materials. The thermal studies of these materials indicate that both of them are highly stable on heating as they retain about 97% of their ion-exchange capacity (i.e.c.) on heating up to 100°C and about 81% on heating up to 200°C.