Showing papers by "Applied Biosystems published in 1984"
TL;DR: A series of automated instruments that use state-of-the-art chemical methods has been developed for high-sensitivity protein sequencing, DNA synthesis and peptide synthesis and their use for the study of a variety of biologically interesting problems.
Abstract: A series of automated instruments that use state-of-the-art chemical methods has been developed for high-sensitivity protein sequencing, DNA synthesis and peptide synthesis. These intruments have been integrated into a centralized microchemical facility in order to promote their use for the study of a variety of biologically interesting problems. This facility has as one of its major functions the development of new chemistries and instrumentation for the structural analysis and synthesis of genes and proteins.
141 citations
TL;DR: The techniques used for the characterization of protein and peptide structure have undergone great changes that have improved the speed, reliability, and applicability of the process, and has made possible the structural analysis of a wide variety of scarce but important compounds.
Abstract: The techniques used for the characterization of protein and peptide structure have undergone great changes that have improved the speed, reliability, and applicability of the process. High-performance liquid chromatography and gel electrophoresis have made the purification of proteins and peptides a routine procedure, even when the compound of interest is a minor component of a complex biological mixture. The chemistry and instrumentation used in amino acid analysis and amino acid sequencing now permit the analysis of as little as 5 to 50 picomoles of samples. This represents an increase in sensitivity of more than a thousandfold over the last 10 years and has made possible the structural analysis of a wide variety of scarce but important compounds.
51 citations
TL;DR: With improved coupling efficiencies, solid-phase techniques would provide an alternative to immobilization of peptides in Polybrene films for low picomole level gas-phase sequencing.
36 citations
TL;DR: Carrots, onions, radishes and peas, growing in pots were used as phytometers to characterise the aerial environment in a pear orchard and the bulb diameter of radish and onion was significantly reduced when growing immediately beneath pear trees.
Abstract: Carrots, onions, radishes and peas, growing in pots were placed at various locations in a pear orchard and used as phytometers to characterise the aerial environment. The growth of plants growing beneath the orchard canopy was not significantly less than that of phytometers grown in the open. However, the bulb diameter of radish and onion was significantly reduced when growing immediately beneath pear trees. Radish was selected as a suitable phytometer for further research.
7 citations
TL;DR: In this paper, a system consisting of an array of filtered selenium photovoltaic cells in specially built cosine corrected mounts was used to record the output from these cells at preselected intervals in a form easily accessed by computer.
Abstract: Details of the design and testing of a system, capable of monitoring the spatial and temporal variation in the availability of photosynthetically active radiation for interculture, are outlined. The system consisted of an array of filtered selenium photovoltaic cells in specially built cosine corrected mounts. A data logger was used to record the output from these cells at preselected intervals in a form easily accessed by computer. The system was tested in a discontinous canopy of pear (var: Conference) and found to be satisfactory.
4 citations