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Institution

Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.


Papers
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Journal ArticleDOI
TL;DR: This work has developed an LC-MS/MS diagnostic and routine monitoring method for known defects due to both purine and pyrimidine metabolism in a single analysis and utilizes a reverse phase chromatographic analysis with a detection requiring 17 min of analysis time.
Abstract: Purines and pyrimidines are the basic constituents of DNA and RNA and constitute the basis of at least 50 other important compounds that serve equally vital but separate roles as integral components of intracellular mononucleotide pools. They maintain the supply of these basic components to the different nucleotide pools through an extremely efficient mechanism involving the degradation and recycling of the daily waste products of normal cell turnover. We have developed an LC-MS/MS diagnostic and routine monitoring method for known defects due to both purine and pyrimidine metabolism in a single analysis. Precision tests were made by spiking several urine samples with different creatinine concentrations. For nonspiked low-creatinine urine, intraday precision was in the range of 0.1–9.8% and interday precision was between 1.6 and 14.1%. For nonspiked high-creatinine urine, intraday precision was in the range 0.5–17.2% and interday precision was between 1.5 and 29%. Limit-of-detection (LOD) was in the range 0.1–10 µmol/l and limit-of-quantification (LOQ) in the range of 0.2–15 µmol/l. The current ‘dilute and shoot’ approach monitors many metabolites, and utilizes a reverse phase chromatographic analysis with a detection requiring 17 min of analysis time. Tandem mass spectrometry and isotope dilution technique enable the accurate quantitation of more than 30 metabolites in one analysis. Copyright © 2006 John Wiley & Sons, Ltd.

37 citations

Patent
12 Jan 1993
TL;DR: In this paper, a beam blocker prevents light that is scattered by the capillary walls from reaching the detector, thereby improving the signal-to-noise ratio of the system, and a pair of legs prevent the sample fluid from being exposed by either the exposing light or the collected light outside of the exposing region.
Abstract: A fluorescence flow cell having a capillary through which a sample fluid is moved, an optical source that directs an exposing beam of light through the sample fluid, a collector that collects fluorescent light from the exposed region of the sample and directs such collected light to a detector. A beam blocker prevents light that is scattered by the capillary walls from reaching the detector, thereby improving the signal-to-noise ratio of the system. The beam blocker also has a pair of legs that prevent the sample fluid from being exposed by either the exposing light or the collected light outside of the exposing region. The structure of the components enables the various components to be quickly and accurately assembled and enables easy replacement of the capillary.

37 citations

Patent
22 Mar 2005
TL;DR: A method of using a standard to correct for variability in sample handling, which can include adding a template of known concentration to an assay comprising a sample, preamplifying the assay, amplifying the assays, collecting data during the amplifying, and correcting the data using a comparison of data collected from the template to the sample as mentioned in this paper.
Abstract: A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.

37 citations

Journal ArticleDOI
TL;DR: The proposed assay, developed and validated for the quantitation of vincristine in human plasma by liquid chromatography/tandem mass spectrometry with atmospheric pressure chemical ionization with on-line solid-phase extraction, is suitable for pharmacokinetics investigations and clinical therapeutic drug monitoring especially in pediatric cancer patients.
Abstract: A simple and rapid method has been developed and validated for the quantitation of vincristine in human plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS) with atmospheric pressure chemical ionization using on-line solid-phase extraction. The method uses vinblastine as internal standard and the sample preparation is limited just to a plasma protein precipitation step. Further sample clean-up is carried out on-line through a perfusion column preceding an analytical phenyl LC column, the latter directly connected to the mass spectrometer. Quantitation is performed in multiple reaction monitoring mode using the transitions of m/z 825.3 --> 765.3 and 811.3 --> 751.3 for vincristine and vinblastine respectively. The assay was linear (r2 > or =0.99) in a concentration range from 0.1 to 500 ng/mL. Carry-over, measured on the experimental set-up, was less than 0.04%. Recovery for vincristine and the internal standard was within 90-95%. The intra-day and inter-day assay precision ranged from 1.2% to 6.8% RSD while mean percentage deviation from nominal value ranged from 0.01% to 6.1%. The proposed assay was found suitable for pharmacokinetics investigations and clinical therapeutic drug monitoring especially in pediatric cancer patients.

37 citations

Patent
28 Dec 2006
TL;DR: In this article, a system and methods for integrating laboratory instrumentation and applications to provide a unified control and coordination architecture under a common interface is presented, where mechanisms for detection of various hardware and software components are automatically recognized and incorporated into a centralized control system that provides live monitoring of the operational status of available components.
Abstract: A system and methods for integrating laboratory instrumentation and applications to provide a unified control and coordination architecture under a common interface. The system provides mechanisms for detection of various hardware and software components wherein the individual functionalities and input/output data types for each component are automatically recognized and incorporated into a centralized control system that provides live monitoring of the operational status of available components.

37 citations


Authors

Showing all 1521 results

NameH-indexPapersCitations
Richard A. Gibbs172889249708
Friedrich C. Luft113109547619
Alexander N. Glazer7120821068
Vineet Bafna6823642574
Kevin R. Coombes6330823592
Darryl J. Pappin6117029409
Mark D. Johnson6028916103
György Marko-Varga5640912600
Paul Thomas5612844810
Gerald Zon5525611126
Michael W. Hunkapiller5113029756
Bjarni V. Halldorsson5114513180
David H. Hawke501579824
Ellson Y. Chen507128836
Sridhar Hannenhalli4916221959
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20182
20171
20164
20152
20147
201313