Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.

Direct Detection and Identification of African Trypanosomes by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes

Abstract: We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

An Optimized Protocol for In Situ Hybridization Using PCR-Generated 33P-Labeled Riboprobes

Abstract: This report describes in detail an in situ hybridization protocol that has been optimized to produce consistent, sensitive results across a wide range of probe constructs and tissue types. Specific advances incorporated into this protocol include (1) the use of PCR gene fragments to generate cRNA probes, (2) the use of 33P-labeled probes and (3) the use of ultrafiltration microconcentrators to purify the probe and eliminate background. The use of 33PCR to generate RNA probes and the use of 33P-labeled probes in in situ hybridization have been described previously; however, this protocol is unique because it combines these techniques in a simple, streamlined procedure that includes the use of microconcentrators to purify the probe. This protocol is particularly appropriate for high-volume in situ hybridization laboratories using a wide variety of probe constructs and tissues.

Plasma clearance and immunologic properties of long-acting superoxide dismutase prepared using 35,000 to 120,000 dalton poly-ethylene glycol.

Abstract: Some biological properties of bovine and recombinant human Cu, Zn superoxide dismutase (bSOD and rhSOD)-poly-ethylene glycol (PEG) adducts prepared by coupling 1–9 strands of high molecular weight PEG (35,000–120,000 daltons) are compared to SOD adducts coupled with 7 or 15 strands of low molecular weight PEG (5,000 daltons).