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Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.


Papers
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Journal ArticleDOI
TL;DR: A simple (two-reagent) real-time PCR assay to quantify P. marinus (PMAR) and Perkinsus spp.
Abstract: Several molecular diagnostic assays have been developed in an attempt to replace the traditional Ray's Fluid Thioglycollate Medium (RFTM) assay for detection and quantification of Perkinsus marinus in oysters. Real-time PCR technology is a state-of-the-art method currently used to diagnose disease intensity in vertebrates. We developed a simple (two-reagent) real-time PCR assay to quantify P. marinus (PMAR) and Perkinsus spp. (PERK) in oysters, using TaqMan® assays designed with Minor Groove Binder (MGB) probes on an Applied Biosystems 7500 Real-Time PCR System. Both PERK and PMAR assays demonstrate strong correlations (R2 ≥ 0.99) between parasite cell density and real-time PCR threshold cycle (CT) with amplification efficiencies ≥99%. The PERK assay results in similar amplification plots for the three species tested (P. marinus, P. olseni and P. chesapeaki), whereas the PMAR assay detects only P. marinus. A strong correlation (R2 > 0.90) was found between infection level determined by the tradit...

34 citations

Journal ArticleDOI
TL;DR: High-performance liquid chromatography coupled with atmospheric pressure photoionization-mass spectrometry (HPLC/APPI-MS) and principal components analysis (PCA) led to the hypotheses that sphingolipids in the SC of desert sparrows have longer carbon chains in the fatty acid moiety and are more polar than those found in mesic sp Sparrows.
Abstract: Because cutaneous water loss (CWL) represents half of total water loss in birds, selection to reduce CWL may be strong in desert birds. We previously found that CWL of house sparrows from a desert population was about 25% lower than that of individuals from a mesic environment. The stratum corneum (SC), the outer layer of the epidermis, serves as the primary barrier to water vapor diffusion through the skin. The avian SC is formed by layers of corneocytes embedded in a lipid matrix consisting of cholesterol, free fatty acids and two classes of sphingolipids, ceramides and cerebrosides. The SC of birds also serves a thermoregulatory function; high rates of CWL keep body temperatures under lethal limits in episodes of heat stress. In this study, we used high-performance liquid chromatography coupled with atmospheric pressure photoionization-mass spectrometry (HPLC/APPI-MS) to identify and quantify over 200 sphingolipids in the SC of house sparrows from desert and mesic populations. Principal components analysis (PCA) led to the hypotheses that sphingolipids in the SC of desert sparrows have longer carbon chains in the fatty acid moiety and are more polar than those found in mesic sparrows. We also tested the association between principal components and CWL in both populations. Our study suggested that a reduction in CWL found in desert sparrows was, in part, the result of modifications in chain length and polarity of the sphingolipids, changes that apparently determine the interactions of the lipid molecules within the SC.

34 citations

Journal ArticleDOI
TL;DR: In this article, the effects of sample exposure to UV laser irradiation on the matrix-assisted laser desorption-ionization (MALDI) mass spectra of different proteins are reported.

34 citations

Journal ArticleDOI
TL;DR: Capillary electrophoresis‐electrospray ionization‐mass spectrometry (CE‐ESI‐MS) has been used to detect naturally occurring nucleotides and to study the metabolism of lamivudine in the human hepatoma cell line Hep G2.
Abstract: We report the use of capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) for the determination of antiretroviral dideoxynucleosides (ddNs), their nucleotides, and a set of ribonucleosides and ribonucleotides. A CE system for separation of most commonly used antiretroviral ddNs has been developed based on a basic buffer with a volatile electrolyte suitable for ESI-MS detection in an untreated capillary column. Positive and negative ionization modes are investigated and compared for sensitive and stable electrospray performance. A 14-compound mixture of nucleosides and nucleotides is profiled in a single capillary zone electrophoresis separation with a distinct elution order: electroosmotic flow, ddNs, mononucleotides, dinucleotides, and trinucleotides in less than 18 min. The fragmentation pathways of the nucleosides and nucleotides in ESI-MS have been interpreted. Concentration limits of detection are 100 to 200 nM with an injection volume of approximately 10 nL. This technique has been used to detect naturally occurring nucleotides and to study the metabolism of lamivudine (3TC) in the human hepatoma cell line Hep G2. 3TC and its metabolites 3TC-monophosphate, 3TC-diphosphate, and 3TC-triphosphate were detected after 10 h of incubation of 3TC with the cells.

33 citations

Patent
04 Sep 2009
TL;DR: In this article, the 3' terminal region of the primers was modified to reduce the formation of primer-dimers during amplification reactions, and various methods of use thereof were discussed.
Abstract: Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.

33 citations


Authors

Showing all 1521 results

NameH-indexPapersCitations
Richard A. Gibbs172889249708
Friedrich C. Luft113109547619
Alexander N. Glazer7120821068
Vineet Bafna6823642574
Kevin R. Coombes6330823592
Darryl J. Pappin6117029409
Mark D. Johnson6028916103
György Marko-Varga5640912600
Paul Thomas5612844810
Gerald Zon5525611126
Michael W. Hunkapiller5113029756
Bjarni V. Halldorsson5114513180
David H. Hawke501579824
Ellson Y. Chen507128836
Sridhar Hannenhalli4916221959
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20182
20171
20164
20152
20147
201313