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Institution

Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.


Papers
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Journal ArticleDOI
TL;DR: In this article, the authors used 1, 2-cyclohexanediamine-N, N, N' N'-tetraacetic acid (CyDTA) as an chelating agent in a carrier solution.
Abstract: Capillary electrophoresis separation of several divalent metal ions, as well as iron(III) and silver(I) was examined with chelating agents. The metal chelates separated in the capillary were measured by on-column UV-absorption detection. When 1, 2-cyclohexanediamine-N, N, N' N'-tetraacetic acid(CyDTA) was used as an chelating agent in a carrier solution (pH 9) the order of the migration time (tm) of metal ions was as follows: Ba2+ Zn2+) and Cd2+

31 citations

Journal ArticleDOI
TL;DR: The ability to collect sufficient quantities of analytes from capillary electrophoresis for subsequent analyses is demonstrated and relevant parameters such as capillary diameter, mass loading, and separation parameters are addressed.

31 citations

Journal ArticleDOI
TL;DR: It is suggested that the AFLP technique has potential as an alternative tool for the molecular epidemiology of E. coli O157:H7 and is almost consistent with those of a pulsed‐field gel electrophoresis (PFGE) study.
Abstract: The amplified fragment-length polymorphism (AFLPTM) technique is based on the selective PCR amplification of restriction fragments. We investigated the utility of AFLP in the molecular subtyping of enterohemorrhagic Escherichia coli serotype O157:H7 isolates. We analyzed a total of 46 isolates of E. coli O157:H7 along with other serotypes, O26:H11, O114:H19 and O119:NT. Isolates of E. coli O157:H7 derived from the same outbreak showed an identical AFLP-banding pattern and were subtyped into the same group, giving results almost consistent with those of a pulsed-field gel electrophoresis (PFGE) study, while other serotypes showed clearly different patterns from those of E. coli O157:H7. These results suggest that the AFLP technique has potential as an alternative tool for the molecular epidemiology of E. coli O157:H7.

31 citations

Journal ArticleDOI
TL;DR: This work found that modified 2-O-Methyl-ribonucleotides were able to dissociate the target miRNA specifically from the RISC complex, and these findings have important implications for understanding the basis for the stability and metabolism of miRNAs in living cells.

31 citations

Journal ArticleDOI
TL;DR: Results were obtained with the three methods, demonstrating the efficiency of PNA probes in the analysis of human sperm, and make PNA-based methodologies very valuable for in situ cytogenetic investigations.
Abstract: Peptide nucleic acids (PNAs) are a relatively new class of synthetic DNA mimics based on a peptide-like backbone. Since their introduction, PNA probes have become established as an efficient variation on the standard FISH procedure for chromosomal identification. In this report we have experimented with centromeric PNA probes on human sperm preparations. Both NaOH and DTT sperm decondensation procedures have been tested and comparative estimates of disomies X, Y and 1 have been performed in sperm from two donors using PNA, FISH and PRINS techniques. Similar results were obtained with the three methods, demonstrating the efficiency of PNA probes in the analysis of human sperm. The fast kinetics, stability and high specificity of PNA probes make PNA-based methodologies very valuable for in situ cytogenetic investigations.

30 citations


Authors

Showing all 1521 results

NameH-indexPapersCitations
Richard A. Gibbs172889249708
Friedrich C. Luft113109547619
Alexander N. Glazer7120821068
Vineet Bafna6823642574
Kevin R. Coombes6330823592
Darryl J. Pappin6117029409
Mark D. Johnson6028916103
György Marko-Varga5640912600
Paul Thomas5612844810
Gerald Zon5525611126
Michael W. Hunkapiller5113029756
Bjarni V. Halldorsson5114513180
David H. Hawke501579824
Ellson Y. Chen507128836
Sridhar Hannenhalli4916221959
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20182
20171
20164
20152
20147
201313