Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.

Automated protein hydrolysis system

Abstract: Protein analysis apparatus has porous sample supports enclosed in holes in a sample slide. Slides are delivered to hydrolysis and derivatizer stations by a rotating turret such that moveable heads at the stations form closed reaction chambers with the slides, sealing around the holes in the slides and enclosing the sample supports. Tubings connected to the heads deliver substances for reaction, derivatization and cleaning. Protein samples applied to sample supports at one position on the turret are delivered sequentially to hydrolysis and derivatizer stations, and operations are computer controlled. Derivatives are automatically delivered for chromatographic analysis, and supports are automatically cleaned with solvents and purge gas after derivatization and before return to a sample load position. Closed reaction chambers formed by the moveable heads can be heated and pressurized as required for processing.

Comparison of packed and capillary columns for practical SFC separations

Abstract: A newly developed polysiloxane-type packing material shows promise for use in SFC. Relatively polar compounds were eluted from a microbore column with good peakshape using a mobile phase consisting of CO2 modified with formic acid and water. The latter combination is an effective modifier suitable for use with pressure programming and FID detection.

Falsely elevated C4-carnitine as expression of glutamate formiminotransferase deficiency in tandem mass spectrometry newborn screening.

Abstract: After the first report by Millington et al.,1 the development of electrospray tandem mass spectrometry (MS/MS) has enabled in recent years the introduction of expanded newborn screening programs in many countries.2,3 This has led to an increase in the capacity to detect rare metabolic disorders during the neonatal period. The increase of the only C4-acylcarnitine, as detected by tandem mass spectrometry in newborn screening, is reported to be related to short chain acyl-CoA dehydrogenase (SCAD) deficiency (butyrylcarnitine) or isobutyryl-CoA dehydrogenase (IBD) deficiency (isobutyrylcarnitine).4 Expanded Newborn Screening Pilot Program using MS/MS is being conducted in three provinces of Tuscany since January 2001, and, being officially mandated by a legislative action, screens all babies born in Tuscany since November 2004 (approximately 30 000/year) for selected acylcarnitines and amino acids. We report on a newborn in which the screening showed an isolated apparent elevation of C4-acylcarnitine usually associated with SCAD or IBD deficiency. A careful evaluation of the results and the subsequent biochemical investigations allowed us to demonstrate that this alteration was due to accumulation of formiminoglutamate (FIGLU). Acylcarnitines and amino acids in all dried blood spots from newborn were analysed as butyl esters with the same methodology described by la Marca et al.5 An Applied Biosystems-Sciex (Toronto, Canada) API 4000 triplequadrupole mass spectrometer equipped with a Turbo V-Spray source was employed. MS and MS/MS spectra were collected in continuous flow mode by connecting the infusion pump directly to the Turbo V-Spray source. A standard solution of 1 ng/μl of each amino acid and acylcarnitine in water : acetonitrile (30 : 70) containing 0.05% formic acid was infused at 5 μl/ min. Measurements on samples were performed by using a Series 1100 Agilent Technologies (Waldbronn, Germany) CapPump coupled to an Agilent Micro ALS autosampler, both fully controlled from the API 4000 data system. Experimental flow rate was 70 μl/ min using water : acetonitrile (30 : 70) containing 0.05% formic acid. The eluent from the column was directed to the Turbo V-Spray probe. The acquired data were processed using the Analyst 1.4.1 proprietary software including the ‘Explore’ option (for spectral interpretation) and the ChemoView software (for quantitative information generation). The child was born from consanguineous Pakistani parents (first cousins) after five uneventful pregnancy. The family history was unremarkable: two brothers and two sisters were normal. The delivery was made by caesarean section because of foetal distress at the 36th week of gestation. At birth, the APGAR score was 9I and 10V, weight 2700 g, length 49 cm, and head circumference 33 cm. Since the first days of life, the infant presented poor sucking and brought up milk. Expanded newborn screening for metabolic diseases by tandem mass spectrometry showed levels of C4-acylcarnitine mildly increased (using a cutoff value of the mean C 2SDs). The physical examination at 20 days of life showed normal cardiac, respiratory and abdominal findings and no dysmorphic features. Increased tendon reflex and mild axial hypotonia with hypertonia of legs were present. EEG showed diffuse paroxystic activity. Abdominal and cerebral ultrasound investigations as well as brain auditory–evoked potentials (BAEPs) and visual-evoked potentials (VEPs) were normal. Fundus oculi, ECG and cardiac ultrasound were normal. Laboratory investigations revealed normocytic anaemia (Hb 10,9, MCV 80,1 fl). Plasma and

Methods, systems and apparatus for light concentrating mechanisms

Abstract: An embodiment relates generally to a method for analysis of a nucleic acid. The method includes providing for a resonant structure configured to couple with one or more fluorescently labeled nucleic acids and directing an excitation light from a source on the resonant structure. The method also includes generating plasmons on the surface of the resonant structure where the analyte is fixed at a point of energy concentration of the resonant structure.