Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.

Methods and apparatus for flow-through hybridization

Abstract: The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.

Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by capillary and microchip electrophoresis for detection of mutations in K-ras gene.

Abstract: We present the rapid single-strand conformation polymorphism (SSCP) analysis by capillary and microchip electrophoresis to detect the mutations in K-ras gene. Parameters that might affect the analysis of mutation in K-ras gene, such as the polymer and the additive in the sieving matrix, have been studied systematically. Under the optimal conditions, the analysis of seven mutants of K-ras gene could be finished within 10 min by capillary electrophoresis (CE). Furthermore, with the wild-type gene as the inner standard, the analysis accuracy of mutations could be improved. In addition, by studying the properties of polymer solutions, the matrix suitable for microchip electrophoresis was found, and the detection of mutations in K-ras gene could be further shortened to 1 min.

Introduction to Polymerase Chain Reaction

Abstract: The polymerase chain reaction (PCR) has become an indispensable tool of molecular biology (1-5). Since its discovery in 1985 the process has found its integration into all research areas involving the use of DNA and RNA. Using this technique, a small starting sample of DNA or RNA can be used to amplify a specific DNA or RNA target over a million-fold in as little as 2 h. This allows for the detection of as little as a single copy of a gene or part of a gene in cells, whether they be from blood, cultured cells, tissue biopsies, chromosomes, or any other biological system that contains DNA or RNA, including archival materials (formalin-fixed, paraffin-embedded).