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TL;DR: Etude de la separation des peptides humains, issue de la digestion enzymatique par la trypsine par laTrypsine.
Abstract: Etude de la separation des peptides humains, issue de la digestion enzymatique par la trypsine
21 citations
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TL;DR: Fluorescent DDRT-PCR increases throughput and obviates the handling of hazardous radioisotopes, and a PCR cycling profile, expected to give improved reproducibility, is described.
Abstract: Differential display reverse transcription PCR (DDRT-PCR) is a procedure used to identify the induction or repression of gene e x pression. In most DDRT-PCR protocols, radioisotopes are incorpo rated during PCR and the cDNA products are detected by autoradio graphy. This report describes the fluorescent labeling of cDNAs and their detection on automated sequencers from PE Applied Biosy s tems. A fluorescent tag can be incorporated into the PCR product b y using either a labeled primer or a labeled dUTP. The fluorescent sig nals are analyzed by GENESCAN ™ software. Fluorescent DDRT PCR increases throughput and obviates the handling of hazardou s radioisotopes. A PCR cycling profile, expected to give improved r e producibility, is also described. Because amplified cDNAs can’t b e recovered from the automated sequencer gel, suggestions are given for the identification and recovery of differentially expressed cDNAs .
21 citations
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09 Apr 2012TL;DR: In this article, an apparatus for detecting labeled beads is provided, which can include one or more irradiation sources disposed for irradiating the detection zones with radiation, at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or multiple detection zones, which have been excited by the radiation.
Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
21 citations
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27 Mar 2004TL;DR: A novel per-base repeat annotation, the $Z$-score, is introduced, from which noise and repeat filtering conditions are explored and dynamic programming-based chaining algorithms are evaluated as context-based filters.
Abstract: Recent sequencing of the human and other mammalian genomes has brought about the necessity to align them, to identify and characterize their commonalities and differences. Programs that align whole genomes generally use a seed-and-extend technique, starting from exact or near-exact matches and selecting a reliable subset of these, called anchors, and then filling in the remaining portions between the anchors using a combination of local and global alignment algorithms, but their choices for the parameters so far have been primarily heuristic. We present a statistical framework and practical methods for selecting a set of matches that is both sensitive and specific and can constitute a reliable set of anchors for a one-to-one mapping of two genomes from which a whole-genome alignment can be built. Starting from exact matches, we introduce a novel per-base repeat annotation, the $Z$-score, from which noise and repeat filtering conditions are explored. Dynamic programming-based chaining algorithms are also evaluated as context-based filters. We apply the methods described here to the comparison of two progressive assemblies of the human genome, NCBI build 28 and build 34 http://genome.ucsc.edu), and show that a significant portion of the two genomes can be found in selected exact matches, with very limited amount of sequence duplication.
21 citations
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22 Mar 2005TL;DR: In this article, a microplate comprising a main body portion having a first surface and an opposing second surface is constructed, each of the plurality of wells being sized to receive an assay therein.
Abstract: A microplate comprising a main body portion having a first surface and an opposing second surface. A plurality of wells are formed in the first surface, each of the plurality of wells being sized to receive an assay therein. A cap portion, formed separate from the main body portion and comprising an integrated identification feature, is connectable with the main body portion.
21 citations
Authors
Showing all 1521 results
Name | H-index | Papers | Citations |
---|---|---|---|
Richard A. Gibbs | 172 | 889 | 249708 |
Friedrich C. Luft | 113 | 1095 | 47619 |
Alexander N. Glazer | 71 | 208 | 21068 |
Vineet Bafna | 68 | 236 | 42574 |
Kevin R. Coombes | 63 | 308 | 23592 |
Darryl J. Pappin | 61 | 170 | 29409 |
Mark D. Johnson | 60 | 289 | 16103 |
György Marko-Varga | 56 | 409 | 12600 |
Paul Thomas | 56 | 128 | 44810 |
Gerald Zon | 55 | 256 | 11126 |
Michael W. Hunkapiller | 51 | 130 | 29756 |
Bjarni V. Halldorsson | 51 | 145 | 13180 |
David H. Hawke | 50 | 157 | 9824 |
Ellson Y. Chen | 50 | 71 | 28836 |
Sridhar Hannenhalli | 49 | 162 | 21959 |