Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.

Base Modification and the Phosphoramidite Approach

Abstract: Oligodeoxynucleotides 18–150 bases in length were synthesized with both 0-(2-cyanoethyl)- and 0-(methyl)-phosphoramidites. After enzymatic degradation of the purified products, base modification and composition were evaluated by HPLC. Additionally, synthesis of 5′-d[GCGCGCTT] with O-(methyl) phosphorus protection generated 3-methylthymidine when thiophenoxide was omitted from the deprotection protocol.

Detection of small RNAS

Abstract: Methods for detecting an RNA such as a small RNA comprising up to about 40 nucleotides are disclosed. The methods comprise forming a ligation mixture comprising a sample suspected of comprising the small RNA, a ligase and a target probe set. The target probe set comprises a first target probe comprising a 3′ portion that hybridizes to the small RNA and a 5′ portion having a first PCR primer target sequence, and a second target probe comprising a 5′ portion that hybridizes to the RNA immediately adjacent to the 3′ end of the first target probe and a 3′ portion having a second PCR primer target sequence. The target probe set hybridizes to the RNA and ligates to form a probe set ligation sequence. A probe set ligation sequence can be amplified and detected using a polymerase chain reaction.

Selecting single-nucleotide polymorphisms for association studies with SNPbrowser software.

Abstract: The design of genetic association studies using single-nucleotide polymorphisms (SNPs) requires the selection of subsets of the variants providing high statistical power at a reasonable cost. SNPs must be selected to maximize the probability that a causative mutation is in linkage disequilibrium (LD) with at least one marker genotyped in the study. The HapMap Project performed a genome-wide survey of genetic variation with over 3 million SNPs typed in four populations, providing a rich resource to inform the design of association studies. A number of strategies have been proposed for the selection of SNPs based on observed LD, including construction of metric LD maps and the selection of haplotype-tagging SNPs. Power calculations are important at the study design stage to ensure successful results. Integrating these methods and annotations can be challenging: the algorithms required to implement these methods are complex to deploy, and all the necessary data and annotations are deposited in disparate databases. Here, we review the typical workflows for the selection of markers for association studies utilizing the SNPbrowser software, a freely available, stand-alone application that incorporates the HapMap database together with gene and SNP annotations. Selected SNPs are screened for their conversion potential to genotyping platforms, expediting the set up of genetic studies with an increased probability of success.

Simulated distillation by packed column supercritical fluid chromatography

Abstract: Etude de la rapidite, de la precision et de la reproductibilite de la methode qui consiste a simuler une distillation par chromatographie fluide supercritique (SFC). La colonne a garnissage utilisee contient de la silice et du siloxane polymere. L'etude est realisee sur du petrole brut provenant de Maljamar (Nouveau Mexique). Les resultats sont compares a ceux obtenus par chromatographie SFC sur colonne capillaire et par analyse thermogravimetrique sous vide. Les trois methodes donnent des resultats comparables