Institution
Applied Biosystems
About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.
Topics: Mass spectrometry, Capillary electrophoresis, Nucleic acid, Oligonucleotide, Tandem mass spectrometry
Papers published on a yearly basis
Papers
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TL;DR: By means of reverse pharmacology screening using a peptide library generated from human hemofiltrate, TIG2 is isolated and identified as the natural ligand of ChemR23 and report the specific molecular form of the bioactive, circulating Tig2, representing the amino‐acid residues 21 to 154 of the 163 amino acid‐containing prepropeptide.
196 citations
TL;DR: The systematic evaluation of important parameters of the labeling protocol is described and optimized labeling conditions using the optimized conditions specific and quantitative labeling was achieved on standard proteins as well as in complex protein mixtures such as a yeast cell lysate.
195 citations
Patent•
04 Feb 1987TL;DR: In this article, a method for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis is presented, which consists of derivatives of fluorescein, 2',7'-dimethoxy-4', 5'-dichlorofluoresceins, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively.
Abstract: A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from the same set. The four sets of dyes of the invention consist of derivatives of fluorescein, 2',7'-dimethoxy-4', 5'-dichlorofluorescein, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively. Dyes from these sets are spectrally resolvable under conditions of gel electrophoresis.
193 citations
TL;DR: A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences, and software that automatically reports mixed-base positions is presented.
Abstract: A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.
192 citations
TL;DR: The TaqMan LS-50B PCR Detection System facilitates the automated and direct detection of polymerase chain reaction (PCR) products and a modified sample preparation protocol using the EnviroAmp kit was chosen for subsequent studies because it was reliable, easy to use and efficient for the isolation of PCR-amplifiable DNA from foods.
191 citations
Authors
Showing all 1521 results
| Name | H-index | Papers | Citations |
|---|---|---|---|
| Richard A. Gibbs | 172 | 889 | 249708 |
| Friedrich C. Luft | 113 | 1095 | 47619 |
| Alexander N. Glazer | 71 | 208 | 21068 |
| Vineet Bafna | 68 | 236 | 42574 |
| Kevin R. Coombes | 63 | 308 | 23592 |
| Darryl J. Pappin | 61 | 170 | 29409 |
| Mark D. Johnson | 60 | 289 | 16103 |
| György Marko-Varga | 56 | 409 | 12600 |
| Paul Thomas | 56 | 128 | 44810 |
| Gerald Zon | 55 | 256 | 11126 |
| Michael W. Hunkapiller | 51 | 130 | 29756 |
| Bjarni V. Halldorsson | 51 | 145 | 13180 |
| David H. Hawke | 50 | 157 | 9824 |
| Ellson Y. Chen | 50 | 71 | 28836 |
| Sridhar Hannenhalli | 49 | 162 | 21959 |