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Institution

Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Capillary electrophoresis. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.


Papers
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Journal ArticleDOI
01 Feb 2010-Genetics
TL;DR: The reference sequence of a boxer is leveraged to construct the first complete linkage map for the domestic dog, which will enable empiric coverage estimates and multipoint linkage analysis, and benefit association, selective sweep, and phylogenetic mapping approaches.
Abstract: We have leveraged the reference sequence of a boxer to construct the first complete linkage map for the domestic dog. The new map improves access to the dog's unique biology, from human disease counterparts to fascinating evolutionary adaptations. The map was constructed with ∼3000 microsatellite markers developed from the reference sequence. Familial resources afforded 450 mostly phase-known meioses for map assembly. The genotype data supported a framework map with ∼1500 loci. An additional ∼1500 markers served as map validators, contributing modestly to estimates of recombination rate but supporting the framework content. Data from ∼22,000 SNPs informing on a subset of meioses supported map integrity. The sex-averaged map extended 21 M and revealed marked region- and sex-specific differences in recombination rate. The map will enable empiric coverage estimates and multipoint linkage analysis. Knowledge of the variation in recombination rate will also inform on genomewide patterns of linkage disequilibrium (LD), and thus benefit association, selective sweep, and phylogenetic mapping approaches. The computational and wet-bench strategies can be applied to the reference genome of any nonmodel organism to assemble a de novo linkage map.

102 citations

Patent
02 Jun 1988
TL;DR: In this paper, a method for capping failure sequences in oligonucleotide synthesis by phosphitylation was proposed, in which a phosphite monoester is reacted with the 5' or 3' hydroxyl of the failure sequence between successive condensation steps in a synthesis procedure to form a 5'' or 3'' phosphite diester.
Abstract: A method is provided for capping failure sequences in oligonucleotide synthesis by phosphitylation. A phosphite monoester is reacted with the 5' or 3' hydroxyl of the failure sequence between successive condensation steps in a synthesis procedure to form a 5' or 3' phosphite diester with the failure sequence. The phosphite diester substituent is inert with respect to subsequent reaction steps in the synthesis of the desired oligonucleotide product.

101 citations

Journal ArticleDOI
TL;DR: Recent progresses in characterizing the role of miRNAs in the maintenance and development of ESCs are reviewed.
Abstract: The unique properties of embryonic stem cells (ESCs) to self-renew indefinitely or to differentiate to any cell type have great potential for clinical applications in regenerative medicine. MicroRNAs (miRNAs) are emerging as important regulators of post-transcriptional gene expression and have been implicated as crucial elements in regulating ESCs. Here, we review recent progresses in characterizing the role of miRNAs in the maintenance and development of ESCs.

100 citations

Journal ArticleDOI
17 Mar 2005-Oncogene
TL;DR: Three representative apoptotic stimuli, that is, serum starvation, a mitochondrial toxin, and a DNA-damaging agent (etoposide), rapidly induce several distinct classes of prosurvival molecules, in particular, Bcl-2/Bcl-XL and superoxide dismutase (SOD; including both MnSOD and Cu/ZnSOD).
Abstract: Most cancer therapeutics fails to eradicate cancer because cancer cells rapidly develop resistance to its proapoptotic effects. The underlying mechanisms remain incompletely understood. Here we show that three representative apoptotic stimuli, that is, serum starvation, a mitochondrial toxin, and a DNA-damaging agent (etoposide), rapidly induce several distinct classes of prosurvival molecules, in particular, Bcl-2/Bcl-XL and superoxide dismutase (SOD; including both MnSOD and Cu/ ZnSOD). At the population level, the induction of these prosurvival molecules occurs prior to or concomitant with the induction of proapoptotic molecules such as Bim and Bak. Blocking the induction using siRNAs of the prosurvival or proapoptotic molecules facilitates or inhibits apoptosis, respectively. One master transcription factor, FOXO3a, is involved in the transcriptional activation of some of these prosurvival (e.g., MnSOD) and proapoptotic (e.g., Bim) molecules. Interestingly, in all three apoptotic systems, FOXO3a itself is also upregulated at the transcriptional level. Mechanistic studies indicate that reactive oxygen species (ROS) are rapidly induced upon apoptotic stimulation and that ROS inhibitors/scavengers block the induction of FOXO3a, MnSOD, and Bim. Finally, we show that apoptotic stimuli also upregulate prosurvival molecules in normal diploid human fibroblasts and at subapoptotic concentrations. Taken together, these results suggest that various apoptotic inducers may rapidly mobilize prosurvival mechanisms through ROS-activated master transcription factors such as FOXO3a. The results imply that effective anticancer therapeutics may need to combine both apoptosis-inducing and survival-suppressing strategies.

99 citations

Patent
27 Mar 2002
TL;DR: The present paper as mentioned in this paper provides amino acid sequences of peptides that are encoded by genes within the Human genome, the GPCR peptides of the present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the peptides, and method of identifying modulators of the PPI peptides.
Abstract: not available for EP1330522Abstract of corresponding document: US2004076951The present invention provides amino acid sequences of peptides that are encoded by genes within the Human genome, the GPCR peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the GPCR peptides and methods of identifying modulators of the GPCR peptides.

99 citations


Authors

Showing all 1521 results

NameH-indexPapersCitations
Richard A. Gibbs172889249708
Friedrich C. Luft113109547619
Alexander N. Glazer7120821068
Vineet Bafna6823642574
Kevin R. Coombes6330823592
Darryl J. Pappin6117029409
Mark D. Johnson6028916103
György Marko-Varga5640912600
Paul Thomas5612844810
Gerald Zon5525611126
Michael W. Hunkapiller5113029756
Bjarni V. Halldorsson5114513180
David H. Hawke501579824
Ellson Y. Chen507128836
Sridhar Hannenhalli4916221959
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20182
20171
20164
20152
20147
201313