Applied Science Private University

About: Applied Science Private University is a education organization based out in Amman, Jordan. It is known for research contribution in the topics: Population & Catalysis. The organization has 4124 authors who have published 5299 publications receiving 116167 citations.

Mathematical Modeling of the Function of Warburg Effect in Tumor Microenvironment.

Abstract: Tumor cells are known for their increased glucose uptake rates even in the presence of abundant oxygen. This altered metabolic shift towards aerobic glycolysis is known as the Warburg effect. Despite an enormous number of studies conducted on the causes and consequences of this phenomenon, little is known about how the Warburg effect affects tumor growth and progression. We developed a multi-scale computational model to explore the detailed effects of glucose metabolism of cancer cells on tumorigenesis behavior in a tumor microenvironment. Despite glycolytic tumors, the growth of non-glycolytic tumor is dependent on a congruous morphology without markedly interfering with glucose and acid concentrations of the tumor microenvironment. Upregulated glucose metabolism helped to retain oxygen levels above the hypoxic limit during early tumor growth, and thus obviated the need for neo-vasculature recruitment. Importantly, simulating growth of tumors within a range of glucose uptake rates showed that there exists a spectrum of glucose uptake rates within which the tumor is most aggressive, i.e. it can exert maximal acidic stress on its microenvironment and most efficiently compete for glucose supplies. Moreover, within the same spectrum, the tumor could grow to invasive morphologies while its size did not markedly shrink.

Effect of physiochemical property of Fe3O4 particle on magnetic lateral flow immunochromatographic assay

Abstract: In this study, Fe3O4 nanoparticles were prepared as bio-probes in lateral flow immunochromatographic immunoassay (LFIA) system for rapid and quantitative detection of Vibrio parahaemolyticus (VP). The relationship was discussed between the assay performance and material characteristics of the probe, including particle size, size distribution and magnetic property. Comparison data with two commercial magnetic particles showed that the bio-probe based on homemade Fe3O4 particle produced the highest magnetic resonance, smaller size and good colloidal stability, a key to obtain good detection performance. Better quantitative signals were achieved by the Fe3O4 particles with small particle size and spherical geometry, that yielded a detection limit of 1 × 105 CFU/mL VP. Whereas a larger diameter greater than 1.0 μm and a broad size distribution obviously impede the fluid dynamic behaviors during the chromatography on the test strip surface, causing a false-positive result. Our study shows that physiochemical property of Fe3O4 particle is key control element for developing high performance LFIA. Magnetic bio-probe in LFIA shows great promise in highly sensitive, rapid qualitative and quantitative on-site test of hazardous substances.