Australian National University

About: Australian National University is a education organization based out in Canberra, Australian Capital Territory, Australia. It is known for research contribution in the topics: Population & Galaxy. The organization has 34419 authors who have published 109261 publications receiving 4315448 citations. The organization is also known as: The Australian National University & ANU.

The honey bee epigenomes: differential methylation of brain DNA in queens and workers.

Abstract: In honey bees (Apis mellifera) the behaviorally and reproductively distinct queen and worker female castes derive from the same genome as a result of differential intake of royal jelly and are implemented in concert with DNA methylation. To determine if these very different diet-controlled phenotypes correlate with unique brain methylomes, we conducted a study to determine the methyl cytosine (mC) distribution in the brains of queens and workers at single-base-pair resolution using shotgun bisulfite sequencing technology. The whole-genome sequencing was validated by deep 454 sequencing of selected amplicons representing eight methylated genes. We found that nearly all mCs are located in CpG dinucleotides in the exons of 5,854 genes showing greater sequence conservation than non-methylated genes. Over 550 genes show significant methylation differences between queens and workers, revealing the intricate dynamics of methylation patterns. The distinctiveness of the differentially methylated genes is underscored by their intermediate CpG densities relative to drastically CpG-depleted methylated genes and to CpG-richer non-methylated genes. We find a strong correlation between methylation patterns and splicing sites including those that have the potential to generate alternative exons. We validate our genome-wide analyses by a detailed examination of two transcript variants encoded by one of the differentially methylated genes. The link between methylation and splicing is further supported by the differential methylation of genes belonging to the histone gene family. We propose that modulation of alternative splicing is one mechanism by which DNA methylation could be linked to gene regulation in the honey bee. Our study describes a level of molecular diversity previously unknown in honey bees that might be important for generating phenotypic flexibility not only during development but also in the adult post-mitotic brain.

Possible gravitational microlensing of a star in the Large Magellanic Cloud

Abstract: THERE is now abundant evidence for the presence of large quantities of unseen matter surrounding normal galaxies, including our own1,2. The nature of this ’dark matter‘ is unknown, except that it cannot be made of normal stars, dust or gas, as they would be easily detected. Exotic particles such as axions, massive neutrinos or other weakly interacting massive particles (collectively known as WIMPs) have been proposed3,4, but have yet to be detected. A less exotic alternative is normal matter in the form of bodies with masses ranging from that of a large planet to a few solar masses. Such objects, known collectively as massive compact halo objects5 (MACHOs), might be brown dwarfs or ‘jupiters’ (bodies too small to produce their own energy by fusion), neutron stars, old white dwarfs or black holes. Paczynski6 suggested that MACHOs might act as gravitational microlenses, temporarily amplifying the apparent brightness of background stars in nearby galaxies. We are conducting a microlensing experiment to determine whether the dark matter halo of our Galaxy is made up of MACHOs. Here we report a candidate for such a microlensing event, detected by monitoring the light curves of 1.8 million stars in the Large Magellanic Cloud for one year. The light curve shows no variation for most of the year of data taking, and an upward excursion lasting over 1 month, with a maximum increase of ∼2 mag. The most probable lens mass, inferred from the duration of the candidate lensing event, is ∼0.1 solar mass.

Despite slow catalysis and confused substrate specificity, all ribulose bisphosphate carboxylases may be nearly perfectly optimized.

Abstract: The cornerstone of autotrophy, the CO2-fixing enzyme, d-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), is hamstrung by slow catalysis and confusion between CO2 and O2 as substrates, an “abominably perplexing” puzzle, in Darwin's parlance. Here we argue that these characteristics stem from difficulty in binding the featureless CO2 molecule, which forces specificity for the gaseous substrate to be determined largely or completely in the transition state. We hypothesize that natural selection for greater CO2/O2 specificity, in response to reducing atmospheric CO2:O2 ratios, has resulted in a transition state for CO2 addition in which the CO2 moiety closely resembles a carboxylate group. This maximizes the structural difference between the transition states for carboxylation and the competing oxygenation, allowing better differentiation between them. However, increasing structural similarity between the carboxylation transition state and its carboxyketone product exposes the carboxyketone to the strong binding required to stabilize the transition state and causes the carboxyketone intermediate to bind so tightly that its cleavage to products is slowed. We assert that all Rubiscos may be nearly perfectly adapted to the differing CO2, O2, and thermal conditions in their subcellular environments, optimizing this compromise between CO2/O2 specificity and the maximum rate of catalytic turnover. Our hypothesis explains the feeble rate enhancement displayed by Rubisco in processing the exogenously supplied carboxyketone intermediate, compared with its nonenzymatic hydrolysis, and the positive correlation between CO2/O2 specificity and 12C/13C fractionation. It further predicts that, because a more product-like transition state is more ordered (decreased entropy), the effectiveness of this strategy will deteriorate with increasing temperature. enzyme mechanisms isotope fractionation transition states

Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis.

Abstract: Background MUC2 mucin produced by intestinal goblet cells is the major component of the intestinal mucus barrier. The inflammatory bowel disease ulcerative colitis is characterized by depleted goblet cells and a reduced mucus layer, but the aetiology remains obscure. In this study we used random mutagenesis to produce two murine models of inflammatory bowel disease, characterised the basis and nature of the inflammation in these mice, and compared the pathology with human ulcerative colitis.