Banaras Hindu University

About: Banaras Hindu University is a education organization based out in Varanasi, Uttar Pradesh, India. It is known for research contribution in the topics: Population & Dielectric. The organization has 11858 authors who have published 23917 publications receiving 464677 citations. The organization is also known as: Kashi Hindu Vishvavidyalay & Benares Hindu University.

Large-Scale Development of Cost-Effective Single-Nucleotide Polymorphism Marker Assays for Genetic Mapping in Pigeonpea and Comparative Mapping in Legumes

Abstract: Single-nucleotide polymorphisms (SNPs, >2000) were discovered by using RNA-seq and allele-specific sequencingapproachesinpigeonpea(Cajanuscajan).FormakingtheSNPgenotypingcost-effective,successful competitive allele-specific polymerase chain reaction (KASPar) assays were developed for 1616 SNPs and referredtoasPKAMs(pigeonpeaKASParassaymarkers).ScreeningofPKAMson24genotypes[23fromcultivated species and 1 wild species (Cajanus scarabaeoides)] defined a set of 1154 polymorphic markers (77.4%) with a polymorphism information content (PIC) value from 0.04 to 0.38. One thousand and ninety-four PKAMs showed polymorphisms between parental lines of the reference mapping population (C. cajan ICP 28 3 C. scarabaeoides ICPW 94). By using high-quality marker genotyping data on 167 F2 lines from the population, a comprehensive genetic map comprising 875 PKAMs with an average intermarker distance of 1.11 cM was developed. Previously mapped 35 simple sequence repeat markers were integrated into the PKAM map and an integrated genetic map of 996.21 cM was constructed. Mapped PKAMs showed a higher degree of synteny with the genome of Glycine max followed by Medicago truncatula and Lotus japonicus and least with Vigna unguiculata. These PKAMs will be useful for genetics research and breeding applications in pigeonpea and for utilizing genome information from other legume species.