Banaras Hindu University

About: Banaras Hindu University is a education organization based out in Varanasi, Uttar Pradesh, India. It is known for research contribution in the topics: Population & Dielectric. The organization has 11858 authors who have published 23917 publications receiving 464677 citations. The organization is also known as: Kashi Hindu Vishvavidyalay & Benares Hindu University.

Darned in 2013: inclusion of model organisms and linking with Wikipedia

Abstract: DARNED (DAtabase of RNa EDiting, available at http://darned.ucc.ie) is a centralized repository of reference genome coordinates corresponding to RNA nucleotides having altered templated identities in the process of RNA editing. The data in DARNED are derived from published datasets of RNA editing events. RNA editing instances have been identified with various methods, such as bioinformatics screenings, deep sequencing and/or biochemical techniques. Here we report our current progress in the development and expansion of the DARNED. In addition to novel database features the DARNED update describes inclusion of Drosophila melanogaster and Mus musculus RNA editing events and the launch of a community-based annotation in the RNA WikiProject.

Chitosan Nanoparticles of 5-Fluorouracil for Ophthalmic Delivery: Characterization, in-Vitro and in-Vivo Study

Abstract: The aim of this investigation was to develop 5-fluorouracil (5-FU) loaded chitosan nanoparticles (CH-DNPs) for ophthalmic delivery. CH-DNPs were fabricated by ionotropic gelation mechanism using chitosan (CH) and a polyanion (TPP). The nanoparticles were smooth and spherical, confirmed by scanning electron microscopy (SEM) and atomic force microscope (AFM). CH/TPP mass ratio and TPP significantly changed the particles size morphology and encapsulation efficiency. The nanoparticles size ranged from approximately 114 to 192 nm and had a positive zeta potential (30±4 mV). The encapsulation efficiency, loading capacity and recovery of DNPs were 8.12-34.32%, 3.14-15.24% and 24.22 to 67% respectively. Physical characterization was done by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD). No interaction was observed in between drug and polymer and crystallinity of drug was not changed in drug loaded nanoparticles. In-vitro release study of DNPs showed diffusion controlled release. Bioavailability study of batch CS9 was studied in rabbit eye and compare to 5-FU solution. 5-FU level was significantly higher in aqueous humor of rabbit eye. Ocular tolerance was studied in the eye of New Zealand rabbits and tested formulation was non-irritant with no sign of inflammation.

Fundamentals and application of ordered molecular assemblies to affinity biosensing

Abstract: Organization of biomolecules in two/three dimensional assemblies has recently aroused much interest in nanobiotechnology. In this context, the development of techniques for controlling spatial arrangement and orientation of the desired molecules to generate highly-ordered nanostructures in the form of a mono/multi layer is considered highly significant. The studies of monolayer films to date have focused on three distinct methods of preparation: (i) the Langmuir–Blodgett (LB) technique, involving the transfer of a monolayer assembled at the gas–liquid interface; (ii) self-assembly at the liquid–solid interface, based on spontaneous adsorption of desired molecules from a solution directly onto a solid surface; and (iii) Layer-by-layer (LBL) self-assembly at a liquid–solid interface, based on inter-layer electrostatic attractions for fabrication of multilayers. A variety of monolayers have been utilized to fabricate biomolecular electronic devices including biosensors. The composition of a monolayer based matrix has been found to influence the activity(ies) of biomolecule(s). We present comprehensive and critical analysis of ordered molecular assemblies formed by LB and self-assembly with potential applications to affinity biosensing. This critical review on fundamentals and application of ordered molecular assemblies to affinity biosensing is likely to benefit researchers working in this as well as related fields of research (401 references).

Analytical solutions of one-dimensional advection-diffusion equation with variable coefficients in a finite domain

Abstract: Analytical solutions are obtained for one-dimensional advection-diffusion equation with variable coefficients in a longitudinal finite initially solute free domain, for two dispersion problems. In the first one, temporally dependent solute dispersion along uniform flow in homogeneous domain is studied. In the second problem the velocity is considered spatially dependent due to the inhomogeneity of the domain and the dispersion is considered proportional to the square of the velocity. The velocity is linearly interpolated to represent small increase in it along the finite domain. This analytical solution is compared with the numerical solution in case the dispersion is proportional to the same linearly interpolated velocity. The input condition is considered continuous of uniform and of increasing nature both. The analytical solutions are obtained by using Laplace transformation technique. In that process new independent space and time variables have been introduced. The effects of the dependency of dispersion with time and the inhomogeneity of the domain on the solute transport are studied separately with the help of graphs.