Showing papers by "Bareilly College published in 2014"

3D-QSAR CoMFA studies on sulfonamide inhibitors of the Rv3588c β-carbonic anhydrase from Mycobacterium tuberculosis and design of not yet synthesized new molecules

Abstract: The human pathogen Mycobacterium tuberculosis contains three β-carbonic anhydrases (CAs, EC 4.2.1.1) in its genome. Inhibition of some of these CAs was shown to modulate the growth of M. tuberculosis. 3D-QSAR Comparative molecular field analyses (CoMFA) were carried out on inhibitors of the enzyme Rv3588c (also denominated mtCA 2). A series of sulfonamides known to inhibit mtCA 2, including some diazenylbenzenesulfonamides, was considered in our study. The predictive ability of the model was assessed using a test set of seven compounds. The best model has demonstrated a good fit having predictive r2 value of 0.93 and cross-validated coefficient q2 value as 0.88 in tripos CoMFA region. Our results indicate that the steric and electrostatic factors play a significant role in mtCA 2 inhibition for the investigated compounds. We proposed nine new not yet synthesized mtCA 2 inhibitors, all of them probably with significantly improved anti-Rv3588c inhibitory activity.

Rotational behavior of Te 120 , 122 , 124

Abstract: Three consecutive Coulomb excitation experiments were performed to measure the reduced transition probabilities in $^{120,122,124}\mathrm{Te}$ by using a $^{58}\mathrm{Ni}$ beam. For $^{120}\mathrm{Te}$ the collectivity was remeasured with high precision yielding a $B(E2;{0}_{\text{g.s.}}^{+}\ensuremath{\rightarrow}{2}_{1}^{+})$ value of 0.666 (20) $\mathrm{e}^{2}\mathrm{b}{}^{2}$. From the $B(E2)$ values connecting higher-lying states, the nuclear structure of $^{120,122,124}\mathrm{Te}$ was determined and shows a rotational behavior quite in contrast with the vibrational structure of the level schemes. The data are compared with different models.

Enhance antimicrobial activity of ZnO nanomaterial׳s (QDs and NPs) and their analytical applications

Abstract: The small scale nanomaterial׳s such as quantum dots and nanoparticles, which have unique properties studied under varying conditions. Various techniques were used in order to best understand the structure of ZnO nanoparticles and quantum dots, which inhibit the growth of Escherichia coli bacteria under dose dependent manner. The results of obtained datas were confirmed with statistical analytical methods. The linear regressive equation data obtained from UV–visible spectra, which give satisfactory results and allows to quickly and easily calculate statistical datas such as: concentration range of analytes, mean, standard deviation, variance and standard analytical error, relative standard deviation, coefficient of correlation, variance of regression line errors in the slope and the intercept, confidence limit for the slope and the intercept. Instead, validation is an important trait measure by any methods due to near to the quality of results. The proposed method validated by specificity, precision, accuracy, limit detection and quantitation. The absorption spectra of sample solution were recorded maximum absorbance for both nanoparticles (NPs) and quantum dots (QDs) at ʎmax 600 nm, this wavelength was suitable and selected for all operational analytical measurements. The size and shape of the prepared NPs and QDs were examined with sophisticated instruments such as FESEM and TEM, whereas crystallinity of the prepared materials was analyzed with X-ray diffraction pattern (XRD).

Serological changes induced by blend of sunset yellow, metanil yellow and tartrazine in swiss albino rat, rattus norvegicus.

Abstract: Objective: The present study was carried out to evaluate the toxic effect of blend of some food colors on Swiss albino rats. Materials and Methods: A blend (1:1:1) of sunset yellow, metanil yellow and tartrazine showed additive effects on serological parameters which indicate that addition of these dye together in food stuff may give rise to more toxic effects than are produced by each dye individually. Animals were divided into four groups (I, II, III, and IV). First group was treated as control and respective group of animals received 25, 50 and 75 mg/kg body weight blend of food colors by gavaging up to 30 days. Results: The serological study showed a decrease in total protein and albumin and an increase in alkaline phosphatase, SGPT and total bilirubin. The results revealed that oral administration of these blend did not affect the body weight gain. Conclusion: The prolonged consumption of the blend may cause adverse effect on human health.

Chemometric modeling of breast cancer associated carbonic anhydrase IX inhibitors belonging to the ureido-substituted benzene sulfonamide class.

Abstract: Ureido-substituted benzene sulfonamides are the most important class of CA inhibitors which significantly inhibited the formation of primary tumors and metastases. Here, we present quantitative structure activity relationships (QSAR) study on a pool of 27 such inhibitors. A heuristic algorithm selected the best multiple linear regression (MLR) equation, showing the correlation between the observed values and the calculated values of activity. The calculated values of activity were in good agreement with the experimental values. The novelty of this work consists in not only exploring the structural attributes of bioactive molecules but also in predicting in silico the structures of new compounds which may show antimetastatic activity. The not yet synthesized such molecules (i.e. the prediction set) included many compounds showing a higher computed activity compared to the reported such derivatives, but they have been however not yet assayed.

Role of incomplete fusion of the projectile in the 16 O + 115 In interaction at low energies

Abstract: Complete and incomplete fusion of an ${}^{16}\mathrm{O}$ projectile with an ${}^{115}\mathrm{In}$ target is studied. Forward recoil range distributions of several evaporation residues were measured at 105 MeV beam energy. The recoil catcher activation technique followed by offline gamma ray spectroscopy was used. The disentanglement of the complete and incomplete fusion processes was done in terms of full and partial linear momentum transfer from the projectile to the target nucleus. Results indicate the occurrence of incomplete fusion involving the breakup of ${}^{16}\mathrm{O}$ into ${}^{4}$He + ${}^{12}$C and/or ${}^{8}$Be + ${}^{8}$Be followed by fusion of one of the fragments with the target nucleus ${}^{115}\mathrm{In}$. The complete fusion contributions deduced from the recoil range distribution data were found to be consistent with the predictions of the theoretical model code pace4. An attempt was also made to separate out the relative contributions of complete and incomplete fusion components from the analysis of the measured recoil range distribution data. The study indicates that the incomplete fusion contribution increases with the projectile energy and the mass asymmetry of the interacting partners. The projectile structure effect also plays an important role in the underlying reaction dynamics.

Monitoring of Metal Deposition by Moss Barbula Constricta J. Linn., from Mussoorie Hills in the India

Abstract: The moss Barbula constricta J. Linn. was used as an active bio-monitor for estimation of atmospheric metal deposition and its seasonal trend to assess the metal (Zn, Pb, Cu, Cd) status of Mussoorie city and its adjoining areas. Moss transplants were exposed for four months, each period representing winter, summer and monsoon season data at the distances of 0.5, 1.0 and 3.0 km during 2003 to 2007. Significantly higher levels of Zn, Pb, and Cu were measured in the moss harvested at the end of each season. The values of metals at different distances from the city center were higher than in the background samples treated as control. Patterns of moss analysis for seasonal and annual metal data were closely identical. Values of metals were the highest during summer followed by winter, and the lowest in monsoon. It was found that an increase recorded in the sampling sites in the past 5 years could be due to the rapid economic development and the manifold increase in tourist activities. Results from the present moss surveys make the examination of both spatial and temporal trends of metals possible, and also confirm the validity of moss B. constricta J. Linn. as a reliable bio-monitor, and recommend it for the bio-mapping program. Data from existing surveys of metal concentrations in moss is filling a gap due to very limited information available in this field. In addition, it is an invaluable resource for policy makers in preparing the strategies for mitigation. DOI: http://dx.doi.org/10.5755/j01.erem.67.1.1750

First report of the moss Rhynchostegiella divaricatifolia (Renauld & Cardot) Broth. from Western Himalayan region of India

Abstract: Study of the bryoflora of the Western Himalayas revealed for the first time the presence of the moss taxon Rhynchostegiella divaricatifolia from the Kumaon region. Earlier this moss was known from the eastern part of the country, i.e. Darjeeling (Sikkim), and was considered unique to that region. The present study describes the extended range of distribution from the Eastern Himalayan to the Kumaon hills of India in an area of 21,035 km(2). Rhynchostegiella divaricatifolia is characterised by a twisted half turn leaf apex, leaf cells showing irregular primordial utricle, linear, irregularly rhomboid. The moss plants are soft, large, glossy, and yellow-green in lax tufts.

Mass-Asymmetry effects in heavy ion reactions: Complete fusion Vs incomplete fusion

Abstract: The excitation functions for 59 Co( 12 C,pn) 69 Ge, 59 Co( 12 C,2pn) 68 Ga, 59 Co( 12 C,p4n) 68 Ge, 59 Co( 12 C,�p4n) 62 Zn reactions for 12 C+ 59 Co projectile-target system have been measured for the first time. The recoil catcher technique followed by o ff-line -ray spectroscopy with HPGe detectors was used. The measured excitation functions are compared with the calculations based on the available statistical model codes. The probability of incomplete fusion is found to increase with the projectile energy. More- over, an attempt has been made to observe the impact of mass-asymmetry on incomplete fusion probability. The effect of �-break-up energy (Q�) on the ICF probability is also studied with the available data.

Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica.

Abstract: Background: The study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of Fasciola gigantica experimentally infected rabbits. Methods: Copro Hyper Immune Serum (HIS) and Excretory-Secretory Hyper Immune Serum (ES HIS) antigens were used in a sandwich ELISA for the detection of F. gigantica antigens in feces of 12 rabbits experimentally infected with different doses of F. gigantica encysted metacercariae (EMC) (10, 25 and 30 EMC). The relation between time of appearance of coproantigens in feces and anti- Fasciola antibodies in serum was evaluated. Results: The earliest diagnostic coproantigen was recorded at 21 st , 25 th and 28 th day post-infection (p.i.) in groups of rabbits infected with 30, 25 and 10 F. gigantica EMC respectively. Both HIS and ES HIS were able to detect coproantigens in feces of rabbits infected with 30 EMC at day 21 p.i. The appearance of F. gigantica coproantigens in feces of infected rabbits was concurrent to the appearance of anti- Fasciola antibodies in blood (3 rd week p.i.). However, coproantigen has specific ability for direct assessment of active infection with minimal cross-reaction with other heterologous parasitic infections. Conclusion: The findings hold promise for a more accurate diagnostic technique in the near future for suspected Fasciola infection.

The Use of Carbonaceous Nanomembrane Filter for Organic Waste Removal

Abstract: Nanotechnology has great impact on the present scenario of wastewater treatment because of their particle size and surface area. Nanomaterials such as metals, metal oxide, low cast adsorbent, nanocatalyst, organic macromolecules composite, and other compounds such as natural organic matter, fullerenes, dendrimers, dyes, chitosan, enzyme, various shaped nanostructures such as nanorods, nanowires and nanotubes (both organic and inorganic) ultrathin films and nanomembrane are used in wastewater treatment. Nanotechnology is an advanced technology used for the cleanup of contaminated water by nanocatalysts, nanofilteration or membrane filtration, and the adsorption of pollutants via nanoscale materials. The use of carbonaceous nanomaterial membranes can serve as high capacity and selective sorbents for organic solutes in aqueous solutions, which can solve wastewater treatment problems. The present chapter initially describes the possible application areas of nanomaterials in wastewater treatment as well as its uses as nanomembrane filter.

In-vitro antidermatophytic activity of essential oil of Psidium guajava (Linn.)

Abstract: Dermatophyte with the ability to digest keratine invade and therve on keratinized of human and animals. In the present study, the activity of essential oil of Psidium guajava (Linn.) was evaluated against four selected dermatophytes, namely Microsporiumcanis, Trichophytomruburum, T. verrucossum, T. tonsurans. Dermatophyteswere isolated with the infected skin, scalp, nail and genital organs of patients from districts hospital, Bareilly. Griseofulvin was used as a standard antifungal drug against the test dermatophytes. Oil was extracted through clevenger’s apparatus. Maximum inhibition zone was reported 69 mm against T. verrucossum followed by 60 mm Trichophytomruburum, 48 mm Trichophytomruburum and 45mm Microsporiumcanis. All five concentrations of oil showed excellent inhibitory effect against all test dermatophytes as compared to standard antifungal used.

Variability in response of Chickpea (Cicer arietinum) to salt stress on enzymatic activity

Abstract: Soil salinity adversely affects majority of biochemical processes in plants. In the present study, the enzymatic activity at different levels of electrical conductivity in soil was studied in Chickpea (Cicer arietinum), in order to understand the mechanism of salt tolerance and evaluate the effects on the yield. Salinity was found to inhibit the activity of both Nitrate reductase as well as total nitrogen. Whereas nitrite reductase and nitrogenase were observed to be incensed in their activity. The study with sodium chloride salinity was chosen as the North belt of Uttar Pradesh (India) was found saline. Saline ground water and soils are dominated by either chloride or sulphate salts. The chemical analysis of soil and plant samples collected from a range of sites at Rohilkhand, India indicated that the enzyme activity in Chickpea was more sensitive to chloride-dominated saline water irrigation as compared with sulphate-dominated.