Showing papers by "Baylor College of Medicine published in 1970"
TL;DR: Synthetic L-2-pyrrolidone-5-carboxylyl- L-histidyl-L-proline amide has physicochemical and biological characteristics which are quantitatively and qualitatively indistinguishable from those of the natural substance isolated in this laboratory.
Abstract: Evidence is presented for the homogeneity of a preparation of hypothalamic TRF of ovine origin and its molecular structure has been established as 2-pyrrolidone-5-carboxylyl-histidyl-proline amide. Synthetic L-2-pyrrolidone-5-carboxylyl-L-histidyl-L-proline amide has physicochemical and biological characteristics which are quantitatively and qualitatively indistinguishable from those of the natural substance isolated in this laboratory.
346 citations
TL;DR: This method of treatment appears safe in patients with compromised cardiac reserve, and effective even in Patients with moderate renal insufficiency, however, the careful replacement of water and electrolyte losses (sodium, potassium and magnesium), however, is essential if this therapy is to be safe.
Abstract: Eight patients with serum calcium values ranging between 12.3 and 18.4 mg per 100 ml were treated with large doses of furosemide intravenously. Their urinary losses of water, sodium and po...
231 citations
TL;DR: The class and fatty acid compositions of the phospholipids from whole retinas of pig, sheep, human, dog, bovine and rabbit were determined in this paper, and each species has nearly identical composition.
226 citations
TL;DR: Determination in quadruplicate of phosphorus in spots after two-dimensional TLC is shown to be a rapid, reproducible procedure for accurate analysis of plasma phospholipid composition, standard deviations for major components being in the 1–2% range.
175 citations
TL;DR: The results suggest that lymphocytotoxic antibodies develop during the course of systemic lupus erythematosus.
Abstract: WE have observed lymphocytotoxic antibodies in sera of patients with connective tissue disorders. The antibodies are cytotoxic to lymphocytes from unrelated random donors using the microcytotoxicity technique1,2. The initial studies were done on 161 coded stored sera submitted to the clinical laboratory for routine immuno-electrophoresis, and confirmatory studies were done on forty-seven coded sera selected from patients followed in the clinic for connective tissue disorders. Results of testing of both series of sera are summarized in Table 1 and show that 74 per cent of all sera tested from patients with systemic lupus erythematosus and 14 per cent of sera from patients with rheumatoid arthritis contained lymphocytotoxic reactivity. Appropriate controls indicated that the cytotoxic effect of lupus sera was dependent on complement; fractionation of one lupus serum by anion exchange chromatography indicated that the cytotoxic factor resides among the IgG globulins. Because cytotoxic antibodies are not present in sera of normal persons except in some cases of immunization by multiple transfusions3,4, pregnancy5,6 or organ transplantation7,8, the results suggest that lymphocytotoxic antibodies develop during the course of systemic lupus erythematosus.
166 citations
TL;DR: Extraction of nucleolar RNA at 65 °C results in the liberation from nucleolar 28 s RNA of a hitherto undescribed low molecular weight RNA referred to as 8 s (28 s) RNA, as well as a previously described U3 RNA and a 7 s RNA.
161 citations
TL;DR: Intravenous administration of synthetic TRF in doses of 250–750 μg stimulates a rise in plasma HTSH levels in normal individuals, and one of two euthyroid patients with a sella tumor appeared to respond suboptimally to TRF.
Abstract: Intravenous administration of synthetic TRF in doses of 250–750 μg stimulates a rise in plasma HTSH levels in normal individuals. No rise in plasma HTSH occurs following 500–750 μg of TRF in subjects on 0.3 mg of Lthyroxine or in patients with panhypopituitarism. One of two euthyroid patients with a sella tumor appeared to respond suboptimally to TRF. It is condluded that synthetic TRF is a convenient means for testing the integrity of the human pituitary for TSH secretion.
119 citations
TL;DR: It was found that rapid alterations of pH in the range 5.9 to 7.8 significantly influenced the affinity of the reticulum for calcium, and decreasing proton concentration was calculated to be sufficient to effect contraction.
114 citations
TL;DR: The facility of this chemical reaction between dopamine and acetaldehyde in conditions which exist in both plants and animals has been confirmed and tetra-hydroisoquinolines have been demonstrated in animals after administration of methanol.
Abstract: THE Pictet–Spengler reaction is a special example of the Mannich reaction involving condensation of β-aryl-ethylamines with carbonyl compounds1. In the case of phenylethylamines the resulting products would be 1,2,3,4-tetrahydroisoquinolines, which are particularly interesting because they could be formed from endogenous biogenic amines. The synthesis of simple tetra-hydroisoquinolines in physiological conditions which occur in plants was described in 19342. The facility of this chemical reaction between dopamine and acetaldehyde in conditions which exist in both plants3 and animals4,5 has been confirmed. Because acetaldehyde is the primary metabolite of ethanol, it has been suggested that tetra-hydroisoquinolines are formed in mammals after the ingestion of alcohol4–6. Indeed, this reaction forms the basis for the histochemical localization of catecholamines in nerve tissue for which formaldehyde vapour is used as the carbonyl agent. Reaction products of this type have been demonstrated in animals after administration of methanol7.
113 citations
TL;DR: To protect other members of the family from the consequences of ventricular arrhythmia experienced by the irreversibly brain-damaged index patient, a series of physiologic investigations was undertaken on this patient in a search for a suitable prophylactic medical regimen.
Abstract: The family, reported on, have a syndrome of prolonged Q-T interval, potentially fatal episodes of ventricular arrhythmias, and normal hearing. The direct familial transmission demonstrated in this pedigree is consistent with an autosomal dominant mode of inheritance. To protect other members of the family from the consequences of ventricular arrhythmia experienced by the irreversibly brain-damaged index patient, a series of physiologic investigations was undertaken on this patient in a search for a suitable prophylactic medical regimen. The attacks of ventricular arrhythmia were found to be precipitated by increase in systemic pressure, sinus tachycardia, and extrasystoles occurring in the supernormal phase of repolarization (R in T syndrome). Propranolol was demonstrated to be an effective agent in preventing the ventricular arrhythmia in the index case and in other family members. Since administration of the beta-adrenergic stimulator isoproterenol did not produce ventricular fibrillation, it is assumed...
112 citations
Journal Article•
TL;DR: The seroepidemiology of the defective adenovirus-associated satellite viruses was studied by use of a neutralization test that has been shown to be much more sensitive than the complement-fixation (CF) test, suggesting that the type 2-3 complex may be the only naturally occurring satellite serotype which infects man.
Abstract: The seroepidemiology of the defective adenovirus-associated satellite viruses was studied by use of a neutralization test that has been shown to be much more sensitive than the complement-fixation (CF) test. Less than 25% of the subjects in any age group were positive for any type of satellite virus CF antibody. The neutralization test revealed serological evidence of satellite infection in almost 80% of the subjects in some age groups. Studies of geometric mean antibody titers by neutralization showed that antibody against type 1 and the type 2-3 complex reached peak levels at 2 to 3 years of age; antibody against types 2 and 3 reached mean titers of 500 to 800, whereas mean antibody levels of only 200 were found for type 1. Type 4 antibodies were rarely noted in human sera. In a companion study in monkeys, monospecific responses to types 1 and 4 were found only in rhesus and green monkey sera, respectively. These data suggest that the type 2-3 complex may be the only naturally occurring satellite serotype which infects man and that antibodies noted against types 1 and 4 may be the result of cross-reactions. No satellite viruses were isolated from throat or rectal swabs of 100 children between 6 months and 3 years of age. However, the patterns of adenovirus and enterovirus isolations from these specimens were typical of previous studies in this laboratory.
TL;DR: The phospholipid class composition and the fatty acid composition of the individualospholipids of mature bovine and rabbit whole retina were determined and phosphatidyl inositols are characterized by high levels of 18:0 and 20:4.
TL;DR: It is concluded that the existence of peripheral vestibuIar labyrinth is essential for the maintenance of dynamic equilibrium when the condition requires advanced psychological and/or physical skill.
Abstract: The maintenance of dynamic equilibrium in the squirrel monkey, measured by squirrel monkey rail test, was investigated after unilateral, bilateral simultaneous, and bilateral non-simultaneous labyr...
TL;DR: It is suggested that food deprivation acts to increase the probability of the subject's detecting the cues and subsequently making a correct response, and the frontal-dominant potential may be the electrical sign of activation of a basic mechanism of selective attention subserved by non-specific thalamo-cortical pathways.
TL;DR: Patients with unequivocal Turner stigmata and cardiovascular disease and chromosomal constitution were studied and coarctation of the aorta occurred in 11 of 16 patients with XO Turner syndrome but not in any of the patients with Turner phenotype or Turner mosaic constitution.
Abstract: Forty patients with unequivocal Turner stigmata and cardiovascular disease were studied. Every patient had complete documentation of both his cardiovascular disease and chromosomal constitution by physical examination, electrocardiogram, roentgenogram and cardiac catheterization, chromosome preparation and buccal mucosal smear. Two clusters of cardiovascular lesions were observed. Coarctation of the aorta occurred in 11 of 16 patients with XO Turner syndrome but not in any of the patients with Turner phenotype or Turner mosaic constitution. Pulmonic stenosis was present in 21 of 24 patients with Turner phenotype and Turner mosaic constitution but was not encountered in those with XO Turner syndrome.
TL;DR: Oxidative phosphorylation and calcium transport were measured in mitochondria isolated from cardiomyopathic hamster hearts and the magnitude of the changes appear to be related to the severity of failure.
TL;DR: A revised model is presented for the conversion reactions involved in formation of ribosomal RNA from nucleolar precursors based on hybridization studies carried out with RNAs and DNA obtained from nucleoli of Novikoff hepatoma ascites cells.
TL;DR: A working model of nucleolar 45 S RNA is presented in which the 28 S nucleolar RNA is placed at the 5' end of the molecule, in good agreement with ratios derived from hybridization studies and equilibrium sedimentation studies.
TL;DR: The 4.5 S RNA was initially obtained from nuclear 4 to 7 S RNA of Novikoff hepatoma cells by electrophoresis on preparative polyacrylamide gels and had a purity of approximately 90% as shown by terminal analysis.
TL;DR: A small, portable, special-purpose device is described which accomplishes automatic on-line evaluation and graphing of a subject's sleep status and considers EOG information to permit detection of the REM state.
TL;DR: Even though infectious virus yields were 10,000-fold lower than those obtained in the presence of arginine, immunofluorescent (IF) and complement-fixing (CF) antigens were detected; however, in the absence ofArginine both IF and CF antigen were found primarily in the cytoplasm of the infected cells.
TL;DR: Ouabain binding in the presence of ATP, Mg2+, Na+ and K+ was shown to be time-dependent and a direct temporal correlation between ouABain binding and inhibition of specific activity of the enzyme system was demonstrated.
TL;DR: In a double-blind comparison of the therapeutic effect of amantadine in a natural outbreak of Hong Kong influenza in January 1969 near Houston, Texas, the decrease in titers of virus in throat swabs from 12 treated patients during the first 10 hours of treatment was appreciably greater than similar titers from 16 untreated patients as mentioned in this paper.
Abstract: In a double-blind comparison of the therapeutic effect of amantadine in a natural outbreak of Hong Kong influenza in January 1969 near Houston, Texas, the decrease in titers of virus in throat swabs from 12 treated patients during the first 10 hr of treatment was appreciably greater than similar titers of virus from 16 untreated patients (P = 0.06). The titer of shed virus decreased significantly more rapidly (P < 0.05) among amantadine-treated patients ill more than 48 hr before treatment than among five control patients who had been ill more than 48 hr. Cough, sore throat, and nasal obstruction cleared more rapidly in treated patients (P < 0.05), and decline of fever in 6 treated patients sick for less than 48 hr before treatment was more rapid than among 13 untreated patients who had been ill for less than 48 hr. These findings are considered to be consistent with a limited therapeutic effect of the drug.
TL;DR: It is suggested that an aberration of intracellular calcium metabolism is characteristic of congestive heart failure associated with the cardiomyopathic process.
TL;DR: It is proposed that anthranilate-5-phosphoribosylpyrophosphate phosphorIBosyltransferase (PR transferase), not anthranILate synthetase, is the rate-limiting reaction in the tryptophan pathway in B. subtilis.