Showing papers by "BIA Separations (Slovenia) published in 1999"
TL;DR: In this article, the isocratic separation of oligonucleotides in the ion exchange mode on thin GMA-EDMA monoliths in the form of commercially available CIM (Convective interaction media) disks is presented.
66 citations
TL;DR: It was shown that similar peak resolution can be provided in both gradient and isocratic modes, and it is possible to achieve efficient separations of oligonucleotides and peptides in the ion-exchange mode as well as the separation of small hydrophobic molecules in the reversed-phase mode.
Abstract: High-performance membrane chromatography (HPMC) proved to be a very efficient method for fast protein separations. Recently, it was shown to be applicable also for the isocratic chromatography of plasmid DNA conformations. However, no study about the separation of small molecules has been performed until now. In this work, we investigated the possibility of gradient and isocratic HPMC of small molecules with Convective Interaction Media disks of different chemistries and tried to explain the mechanism that enables their separation. We demonstrated that it is possible to achieve efficient separations of oligonucleotides and peptides in the ion-exchange mode as well as the separation of small hydrophobic molecules in the reversed-phase mode. It was shown that similar peak resolution can be provided in both gradient and isocratic modes.
62 citations
Patent•
26 Mar 1999TL;DR: In this paper, a device for the rapid separation and/or conversion of substrates is described, which includes a porous tube (2) disposed inside a tubular sample distributor (3) and a circular sample collector (4) having channel structures on its outer surface is disposed inside the porous tube.
Abstract: A device for the rapid separation and/or conversion of substrates is disclosed herein. The device include a porous tube (2) disposed inside a tubular sample distributor (3). The tubular sample distributor (3) includes channel structures on its inner surface and is disposed inside a tubular body (4). A tubular sample collector (1) having channel structures on its outer surface is disposed inside the porous tube (2). A circular sample distributor (7) is provided having a circumferential portion and radially extending grooves in conjunction with the channel structures of the tubular sample distributor (3). A circular sample collector (8) is also provided having a circumferential portion and radially extending grooves in conjunction with the channel structures of the tubular sample collector (1). The hollow body (4) is arranged between securing means (5) that provide a sample inlet and outlet in communication with the circular sample distributor (7) and circular sample collector (8), respectively.
3 citations