Showing papers by "BIA Separations (Slovenia) published in 2003"
TL;DR: In this paper, the effect of various methacrylate monomers on the porous structure of the monolith was investigated, and the porosity of the porous structures was correlated with the specific surface area, pressure drop, and dynamic binding capacity.
Abstract: Methacrylate-based monoliths are formed during radical copolymerization as a consequence of the precipitation of polymeric chains from the reaction mixture, which consists of monomers, initiator, and the porogenic solvents. The effect of various methacrylate monomers on the porous structure of the monolith was investigated. Although the chemical structure of the monomers significantly affects the size of the pores and the porosity, the mechanism of pore formation in the case of the precipitation during polymerization is preserved. The porous structure was further correlated with the specific surface area, pressure drop, and dynamic binding capacity of the monoliths studied.
67 citations
TL;DR: In this article, the enrichment of virus RNA on short monolithic columns prior to molecular detection of viruses is described, and the results show that it is possible to bind viral RNA on monoliths and concentrate viral nucleic acids from a fairly dilute sample.
63 citations
TL;DR: The Michaelis-Menten constant K(m) and the turnover number k(3) of the immobilized enzymes were found to be flow-unaffected and indicate the absence of a diffusional limitation in immobilized CIM enzyme reactors.
53 citations
TL;DR: It is demonstrated that monolithic columns are useful tools also at an early stage of the purification process of factor IX from human plasma, starting with the eluate after solid-phase extraction with DEAE-Sephadex and after up-scaling experiments.
36 citations
TL;DR: The proposed approach suggested by Yamamoto for transfer of gradient methods between columns of different size can be successfully used for method scale-up on this type of column.
29 citations
TL;DR: The binding capacity for both BSA and soybean trypsin inhibitor increased with increasing temperature and the effect on the dynamic binding capacity of dimers present in the BSA solution has been evaluated and a closed-loop frontal analysis was used to determine the equilibrium binding capacities.
24 citations
TL;DR: In this paper, a mathematical model for the prediction of temporal and spatial temperature distribution during the polymerization of methacrylate-based monolithic columns is introduced, which is described by an unsteady-state heat conduction equation with the generation of heat related to the general kinetics of polymerization.
Abstract: Monolithic stationary phases are becoming increasingly important in the field of liquid chromatography. Methacrylate-based monoliths are produced via free-radical bulk polymerization. The preparation of large-volume monoliths is a major problem because the intensive heat released during polymerization causes distortion of the porous monolithic structure. This work presents experimental measurements of temperature distributions during polymerization in moulds of different sizes and at various experimental conditions. A mathematical model for the prediction of temporal and spatial temperature distribution during the polymerization of methacrylate-based monolithic columns is introduced. The polymerization is described by an unsteady-state heat conduction equation with the generation of heat related to the general kinetics of polymerization. Predictions from the mathematical model are in good agreement with the experimental measurements at different experimental conditions. A method for construction of large-volume monolithic columns is presented and an attempt is made to adopt the developed mathematical model in annular geometry. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 87: 2326–2334, 2003
15 citations
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5 citations