Showing papers by "BIA Separations (Slovenia) published in 2007"
TL;DR: Efficient chromatographic purification of tomato mosaic virus from plant material is described and the purification process was shortened from 5 days to 2 hours, demonstrating the potential of short monolithic column technology for purification and analysis of different viruses.
67 citations
TL;DR: The results of this study indicate that the PolyHIPE structure under given experimental condition is, from a hydrodynamic point of view, to some extent similar to foam structures, though any extrapolation of these results may not provide useful predictions of pressure versus flow relations and further experiments are required.
49 citations
TL;DR: A unique and simple diagnostic scheme for rapid, efficient, and sensitive monitoring of irrigation waters that could also be adopted for other plant, human or animal viruses is proposed.
Abstract: A quantitative RT real-time PCR method was developed for the detection and quantification of Tomato mosaic virus (ToMV) in irrigation waters. These have rarely been monitored for the presence of plant pathogenic viruses, mostly due to the lack of efficient and sensitive detection methods. The newly developed method presented here offers a novel approach in monitoring the health status of environmental waters. ToMV was reliably detected at as low as 12 viral particles per real-time PCR reaction, which corresponds to the initial concentration of approximately 4.2 × 10−10 mg (6,300 viral particles) of ToMV per ml of sample. The sensitivity of the method was further improved by including the Convective Interaction Media® (CIM) monolithic chromatographic columns for quick and efficient concentration of original water samples. Seven out of nine water sources from different locations in Slovenia tested positive for ToMV, after concentrating the sample. Four samples tested ToMV-positive without the concentrating procedure. The presence and integrity of infective ToMV particles in the original sample, as well as in the chromatographic fraction, was confirmed using different methods from test plants, DAS ELISA to electron microscopy and real-time PCR. In this study, we propose a unique and simple diagnostic scheme for rapid, efficient, and sensitive monitoring of irrigation waters that could also be adopted for other plant, human or animal viruses.
45 citations
TL;DR: Interestingly, specific biological activity was increasing with a pore size decrease and was attributed to higher number of contacts between a substrate and immobilized ligand, probably due to restricted access for DNA molecules into the small pores.
36 citations
TL;DR: In this paper, different ion-exchange methacrylate monoliths were tested for the separation of immunoglobulin G (IgG) and IgM. The strong anion exchange column had the highest dynamic binding capacity reaching more than 20 mg of IgM/ml of support.
33 citations
TL;DR: The main leaking compound from DEAE monolith was found to be 3-(diethylamino)-1,2-propanediol and 2,3-dihydroxypropyltrimethylammonium salt for QA monolith, and during repeated 50 cleaning-in-place (CIP) cycles, no changes in chromatographic properties were detected.
31 citations
TL;DR: It has been shown that anion-exchange and affinity chromatography using convective interaction media monolithic columns can represent an efficient complementary technique for human serum albumin and immunoglobulin G removal from human plasma.
30 citations
TL;DR: Enzyme reactor was found to efficiently eliminate RNA contaminants from DNA samples and was active for several weeks of operation and processed 300 column volumes of sample.
28 citations
Patent•
06 Jul 2007TL;DR: A process for the purification of influenza virus or derivative thereof comprising the steps of: providing a source having influenza virus, optionally subjecting the source to a prepurification step; followed by at least one chromatographic step on chromatogram materials selected from the group consisting of porous particles having mean pore sizes of at least 20 nm, perfusion particles, gel-in-a-shell particles, tentacle like particles, membrane adsorbers, and monoliths, with the proviso that sulfuric ester of cellulose or cross-linked polysaccharides
Abstract: A process for the purification of influenza virus or derivative thereof comprising the steps of: providing a source having influenza virus or derivative thereof; optionally subjecting the source to a prepurification step; followed by at least one chromatographic step on chromatographic materials selected from the group consisting of porous particles having mean pore sizes of at least 20 nm, perfusion particles, gel-in-a-shell particles, tentacle like particles, membrane adsorbers, and monoliths; collecting eluting influenza virus or derivatives thereof containing fractions; with the proviso that sulfuric ester of cellulose or cross-linked polysaccharides are excluded.
25 citations
TL;DR: It was found that the two drugs occupy the same class of binding sites on BSA, which indicated that naproxen had an association equilibrium constant of 2.90 x 10(6)M(-1) at pH 7.4 and 39 degrees C for a single binding site.
22 citations
TL;DR: Frontal analysis of anti-NT-proBNP disks revealed the ability of the immunoadsorber to bind up to 250 pmol NT- ProBNP, which is more than sufficient for the analysis of clinical samples and excellent batch-to-batch reproducibility.
Abstract: Immunoadsorbers based on 2.0 × 6.0 mm i.d., epoxy-bearing, methacrylate-based monolithic disks were developed in order to target myoglobin and N-terminal pro-natriuretic peptide (NT-proBNP), two biomarkers involved in cardiovascular disease. In both cases, antibodies were successfully coupled to the polymeric disk material. The developed immunoadsorbers permitted the selective isolation of myoglobin and NT-proBNP from human serum. Myoglobin was successfully isolated and detected from serum samples at concentrations down to 250 fmol μL−1. However, the affinity of the antibodies was not sufficient for the analysis of low-concentration clinical samples. Frontal analysis of anti-NT-proBNP disks revealed the ability of the immunoadsorber to bind up to 250 pmol NT-proBNP, which is more than sufficient for the analysis of clinical samples. Anti-NT-proBNP disks showed good stability over more than 18 months and excellent batch-to-batch reproducibility. Moreover, anti-NT-proBNP disks permitted the isolation of NT-proBNP at concentrations down to 750 amol μL−1 in serum, corresponding to concentrations of strongly diseased patients. Using reversed-phase trapping columns, the detection of NT-proBNP eluted from immunoadsorbers by mass spectrometry was achieved for concentrations down to 7.8 fmol μL−1.
TL;DR: A high-performance liquid chromatography (HPLC) method for the determination of DNA entrapment efficiency in liposomes has been developed that is fast, simple, precise and does not require any kind of DNA labelling in contrast with mostly used methods.
TL;DR: A rapid, robust, and highly reproducible chromatographic method, based on HPLC separation on Convective Interaction Media, is developed to assess the quantity of Atxs in a particular venom sample in order to predict its potential to induce highly protective antiserum in immunized animals.
Abstract: It was confirmed in this study that Vipera ammodytes ammodytes venom samples with a higher content of ammodytoxins (Atxs), basic neurotoxic phospholipases A2, are more lethal, exposing Atxs as one of the major toxic components in the long‐nosed viper venom. In addition, we recently correlated the ability of the venom to produce highly protective antiserum in rabbits with the amount of Atxs in the venom. Here, we developed a rapid, robust, and highly reproducible chromatographic method, based on HPLC separation on Convective Interaction Media to assess the quantity of Atxs in a particular venom sample in order to predict its potential to induce highly protective antiserum in immunized animals.
Patent•
06 Jul 2007TL;DR: A process for the purification of influenza virus or derivative thereof comprising the steps of: providing a source having influenza virus, optionally subjecting the source to a prepurification step; followed by at least one chromatographic step on chromatogram materials selected from the group consisting of porous particles having mean pore sizes of at least 20 nm, perfusion particles, gel-in-a-shell particles, tentacle like particles, membrane adsorbers, and monoliths.
Abstract: A process for the purification of influenza virus or derivative thereof comprising the steps of: providing a source having influenza virus or derivative thereof; optionally subjecting the source to a prepurification step; followed by at least one chromatographic step on chromatographic materials selected from the group consisting of porous particles having mean pore sizes of at least 20 nm, perfusion particles, gel-in-a-shell particles, tentacle like particles, membrane adsorbers, and monoliths; collecting eluting influenza virus or derivatives thereof containing fractions with the proviso that sulfuric ester of cellulose or cross-linked polysaccharides are excluded.